Effects of silicon supply and Sphaerotheca fuliginea inoculation on resistance of cucumber seedlings against powdery mildew

Abstract

Abstract: By the method of hydroponic culture,this paper studied the effects of soluble silicon (Si) supply and Sphaerotheca fuliginea inoculation on the active oxygen metabolism,SiO₂ content and disease resistance of a susceptible cultivar.The results showed that the O₂^-_? producing rate and H₂O₂ and MDA contents of cucumber leaf were increased by Sphaerotheca fuliginea inoculation,but reduced by the inoculation plus Si supply.The inoculation enhanced the catalase (CAT) and peroxidase (POD) activities in plant leaves but decreased their superoxide dismutase (SOD) activity,while inoculation plus Si supply increased the activities of these enzymes.Inoculation increased the scorbate (AsA) and glutathione (GSH) contents in plant leaves.The AsA content in Si-supplied plant leaves was lower than that of Si-deprived ones,whereas the GSH content was in reverse.Whether inoculated or not,the SiO₂ content in Si-supplied plant leaves was significantly higher than that of Si-deprived ones.The disease index was significantly decreased by Si-supply.

Key words: Cucumber powdery mildew, Silicon, Enzyme activity, AsA, GSH

CLC Number:

S436.421

WEI Guoqiang, ZHU Zhujun, LI Juan, YAO Qiuju. Effects of silicon supply and Sphaerotheca fuliginea inoculation on resistance of cucumber seedlings against powdery mildew[J]. Chinese Journal of Applied Ecology, 2004, (11): 2147-2151.

References

[1] Bowen P,Menzies JG,Ehret D,et al.1992.Soluble silicon sprays inhibit powdery mildew development on grape leaves.J Am Soc Hort Sci,117:906～912
[2] Cakmak I,Marschner H.1992.Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,ascorbate peroxidase,and glutathione reductase in bean leaves.Plant Physiol,98:1222～1227
[3] Chen L-F(陈利锋),Ye M-B(叶茂炳),Chen Y-X(陈永幸),et al.1997.The relationship between ascorbic acid and resistance of wheat to scab.Acta Phytophathol Sin(植物病理学报),27(2):113～118(in Chinese)
[4] Cherif M,Benhamou N,Menzies JG,et al.1992.Silicon induced resistance in cucumber plants against Pythium ultimum.Physiol Mol Plant Pathol,41:411～425
[5] Dong Y-M(董毅敏),Xu J-H(徐建华),Guo J-F(郭季芳).1990.The changes of some enzymes activities from cucumber leaves after inoculation with cucumber powdery mildew.Acta Bot Sin(植物学报),32(2):160～164(in Chinese)
[6] El-Zahaty HM,Gullner G,Kiraly Z.1995.Effects of powdery mildew infection of barley on the ascorbate-glutathione cycle and other antioxidatants in different host-pathogen interactions.Phytopathology,85:1225～1230
[7] Fang Z-D(方中达).1998.Research Methods of Phytophathologica.Beijing: Agricultural Press.366～368(in Chinese)
[8] Feng D-X(冯东昕),Li B-D(李宝栋).1998.The role of soluble silicon in disease management of plant.Acta Phytophathol Sin(植物病理学报),28(4):293～297(in Chinese)
[9] Giannopolitis CN,Ries SK.1977.Superoxide dismutase Ⅰ.Occurrence in higher plants.Plant Physiol,59:309～314
[10] Guri A.1983.Variation in glutathione and ascorbic acid content among selected cultivars of Phseolus vulgaris prior to and after exposure to ozone.Can J Plant Sci,63:733～737
[11] Helene V,Tim LW,Christine HF.2000.Early H₂O₂accumulation in mesophyll cells leads to induction of glutathione during the hyper-sensitive response in the barley-powdery mildew interaction.Plant Physiol,123:1289～1300
[12] Liang Y-C(梁永超),Chen X-H(陈兴华),Wang D-P(王大平).1991.Changes in TCA-Si (Trichloroacetic acid soluble silica) content and its distribution in rice plants.J Jiangsu Agric Sci(江苏农业学报),7(4):41～43(in Chinese)
[13] Liang Y-C(梁永超),Sun W-C(孙万春).2002.Resistance of cucumber against anthracnose induced by soluble silicon and inoculated Colletotrichum iagenarium.Sci Agric Sin(中国农业科学),35(3):267～271(in Chinese)
[14] Lin Z-F(林植芳),Li S-S(李双顺),Lin G-Z(林桂珠).1988.The accumulation of hydrogen peroxide in senescing leaves and chloroplasts in relation to lipid peroxidation.Acta Phytophysiol Sin(植物生理学报),14(1):16～12(in Chinese)
[15] Menzies JG,Ehret DL,Glass ADM,et al.1991.Effects of soluble silicon on the parasitic fitness of Spherotheca fuliginea on Cucumis sativus.Phytopathology,81:84～88
[16] Ma Q(马青),Cui H-W(崔鸿文),Qiu G-S(仇贵生).1997.Effects of diniconazole against cucumber powdery mildew.Acta Boreal-Occident Agric Univ(西北农业大学学报),25(1):36～40(in Chinese)
[17] Samuels AL,Glass ADM,Ehret DL,et al.1993.The effect of silicon supplementation on cucumber fruit:Changes in surface characteristics.Ann Bot,72:433～440
[18] Song F-M(宋凤鸣),Ge X-C(葛秀春),Zheng Z(郑重).2001.Changes of glutathione contents in cotton seedlings infected by Fusarium oxysporum sp.Vasinfectum and its relationship to disease resistance.J Zhejiang Univ(Agric & Life Sci)(浙江大学学报·农业与生命科学版),27(6):615～618(in Chinese)
[19] Wang A-G(王爱国),Luo G-H(罗广华).1990.Quantitative relation between the reaction of hydroxylamine and superoxide anionradicals in plants.Plant Physiol Com(植物生理学通讯),6:55～57(in Chinese)
[20] Wang Y-R(王以柔),Zeng S-X(曾韶西),Li X-P(李晓萍).1990.Photooxidation of rice seedlings induced by low temperature.Acta Phytophysiol Sin(植物生理学报),16(2):102～108(in Chinese)
[21] Zhu Z-J(朱祝军),Yu J-Q(喻景权),Gerendas J,et al.1998.Effect of light and nitrogen form on growth and activities of H₂O₂-scavenging enzymes in tobacco.Plant Nutr Fert Sci(植物营养与肥料学报),4(4):379～385(in Chinese)
[22] Jiang M-Y(蒋明义),Yang W-Y(杨文英),Xu J(许江),et al.1994.Active oxygen damage effect of chlorophyll degration in rice seedlings under osmotic stress.Acta Bot Sin(植物学报),36:289～295(in Chinese)
[23] Feng Y-L(冯玉龙),Zhang Y-J(张亚杰),Zhu C-Q(朱春全).2003.Relationship between photo-inhibition of photosynthesis and reactive oxygen species in leaves of poplars suffering root osmotic stress.Chin J Appl Ecol(应用生态学报),14(8):1213～1217(in Chinese)
[24] Wang H-H(王海华),Zeng F-H(曾富华),Kang J(康健),et al.2001.Effect of nickel treatment on H₂O₂ accumulation and induced resistance to Xanthomonas oryzae pv.Oryzae in rice leaf.Acta Phytophysiol Sin(植物生理学报),27(1):61～65(in Chinese)
[25] Cai Z-Q(蔡志全),Cao K-F(曹坤芳),Feng Y-L(冯玉龙),et al.2003.Effect of low nocturnal temperature stress on fluorescence characteristics and active oxygen metabolism in leaves of Garcinia hanburyi seedlings grown under two leaves of irradiance.Chin J Appl Ecol(应用生态学报),14(3):326～331(in Chinese)
[26] Zhou Y-H(周艳虹),Yu J-Q(喻景权),Qian Q-Q(钱琼秋),et al.2003.Effects of chilling and low light on cucumber seedlings growth and their antioxidative enzyme activities.Chin J Appl Ecol(应用生态学报),14(6):921～924(in Chinese)
[27] Zhu Y-Y(朱毅勇),Shen Q-R(沈其荣),Xie X-D(谢学东),et al.1999.Enzymatic activities during the induced systemic resistance of cucumber by K2HPO4.J Nanjing Agric Univ(南京农业大学学报),22(2):50～54(in Chinese)
[28] Yang Y-F(杨艳芳),Liang Y-C(梁永超),Lou Y-S(娄运生),et al.2003.Influences of silicon on peroxidase,superoxide dismutase activity and lignin content in leaves of wheat(Tritium aestivum L.)and its relation to resistance to powdery mildew.Sci Agric Sin(中国农业科学),36(7):813～817(in Chinese)