Effect of sodium phytate on alkaline phosphatase (ALP) activity in intraradical hyphae of AM fungi and development of its extraradical hyphae

Abstract

Abstract: In this paper,a pot experiment with three compartments was installed to study the effect of Na-phytate on the development and metabolic activity of AM fungi.Maize was selected as host plant and inoculated with arbuscular mycorrhizal fungus (Glomus intraradices),and different amounts of Na-phytate were applied into hyphal compartments.The results showed that the absorbed P in the shoots and roots of maize inoculated with AM fungi was 1～2 times higher than that of non inoculated maize.The ALP activity in intraradical hyphae of AM fungi was significantly decreased,and the total hyphal density of AM fungi increased with applied Na-phytate.Observations suggested that the ALP activity in intraradical hyphae of AM fungi and the development of its extraradical hyphae could be regulated by Na-phytate.Additionally,AM fungi could enhance the plant's P absorption and utilization from native soil P and additive Na-phytate.

Key words: AM fungus, Na-phytate, ALP, Extraradical hyphae

CLC Number:

Q949.32

FENG Haiyan, FENG Gu, WANG Jingguo, LI Xiaolin . Effect of sodium phytate on alkaline phosphatase (ALP) activity in intraradical hyphae of AM fungi and development of its extraradical hyphae[J]. Chinese Journal of Applied Ecology, 2004, (6): 1009-1013.

References

[1] Feng G,Song YC,Li XL,et al.2003.Contribution of aruscular mycorrhizal fungi to utilization of organic sources of phosphorus by red clover in a calcareous soil.Appl Soil Ecol,22:139～148
[2] Gryndler M,Hrselova H,Chvatalova I,et al.1998.In vitro proliferation of Glomus fistulosum intraradical hyphae from mycorrhizal root segments of maize.Mycol Res,102:1067～1073
[3] Hamel C,Fyles H,Smith DL.1990.Measurement of development of endomycorrhizal mycelium using three different vital strains.New Phytol,115:297～302
[4] Harinikumar KM,Bagyaraj DJ.1989.Effects of cropping sequence,fertilizers and farmyard manure on vesicular-arbuscular mycorrhizal fungi in different crops over three consecutive seasons.Biol Fertil Soil,7:173～175
[5] Hodge A,Campbell CD,Fitter AH.2001.An arbuscular mycorrhizal fungus accelerates decomposition and acquires nitrogen directly from organic material.Nature,413(6853):297～299
[6] Jakobsen I,Abbott LK,Robosen AD.1992.External hyphae of vesicular-arbuscular mycorrhizal fungi associated with Triflolium subterraneum L.1.Spread of hyphae and phosphorus inflow into roots.New Phytol,120:371～380
[7] Joner EJ,Jakobsen I.1995.Growth and extracellular phosphatase activity of arbuscular mycorrhizal hyphae as influence by soil organic matter.Soil Biol Biochem,27(9):1153～1159
[8] Kabir Z,O'Halloran IP,Fyles JW,et al.1998.Dynamics of the mycorrhizal symbiosis of corn(Zea may L.):Effects of host physiology,tillage practice and fertilization on spatial distribution of extra-radical mycorrhizal hyphae in the field.Agric Ecosyst Environ,68:151～163
[9] Kurle JE,Pfleger FL.1994.The effects of cultural practices and pesticides on VAM fungi.In:Pfleger FL,Lindermann RG,eds.Mycorrhizae and Plant Health.St Paul.Minnesota:APS Press.101～130
[10] Li XL,Marschner H,George E.1991.Extension of the phosphorus depletion zone in VA mycorrhizal white clover in a calcareous soil.Plant soil,136:41～48
[11] Rilling MC,Steinberg PD.2002.Glomalin production by an arbuscular mycorrhizal fungus:A mechanism of habitat modification.Soil Biol Biochem,34:1371～1374
[12] Sander FE.1975.The effect of foliar-applied phosphorus on the mycorrhizal infection of onion roots.In:Sander FE,Mosse B,Tinker PB eds.Endomycorrhizas.London:Academic Press.261～276
[13] Song Y-C(宋勇春).2000.Phosphatase activity in the mycorrhizosphaere and its role in the utilization of organic phosphate by plants.Ph.D.Dissertation.Beijing:China Agricultural University.69～76(in Chinese)
[14] Su Y-B(苏有波),Wang H(王贺),Zhang J-L(张俊玲),et al.1998.Effect of arbuscular mycorrhiza on phosphatase activity in the rhizosphere of clover.Plant Nutr Fertil Sci(植物营养与肥料学报),4(3):264～270(in Chinese)
[15] Thomson BD,Robson AD,Abbott LK.1991.Soil mediated effects of phosphorus supply on the formation of mycorrhizas by Schtellispora calospora(Nicol.& Gerd.) Walker & Sanders on subterranean clover.New Phytol,118:463～469
[16] Tisserant B,Brenac V,Requena N,et al.1998.The detection of Glomus spp.(arbuscular mycorrhizal fungi) forming mycorrhizas in three plants,at different stages of seedling development,using mycorrhiza-specific isozymes.New Phytol,138:225～239
[17] Tisserant B,Gianinazzi S,Gianinazzi-pearson V,et al.1996.Relationships between lateral root order,arbuscular mycorrhiza development and the physiological state of the symbiotic fungus in Platanus acerifolia.Can J Bot,74:1947～1955
[18] Tisserant B,Gianinazzi-pearson V,Gianinazzi S,et al.1993.In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections.Mycol Res,97(2):245～250
[19] Trappe JM,Molina R,Castellano M.1984.Reaction of mycorrhizal fungi and mycorrhiza formation to pesticides.Ann Rev Phytophthol,22:331～359
[20] Trouvelot A,Kough JL,Gianiazzi-Pearson V.1986.Mesure du taux de mycorhization VA d'un système radiculaire.Recherche de methods d'estimation ayant une signification fonctionnelle.In:Gianiazzi-Pearson V,Gianiazzi S eds.Physiological and Genetical Aspects of Mycorrhizae.Paris:INRA Press.217～221