Abstract: By the method of liquid culture, this paper studied the effects of different P and Pb doses on the biomass production of three strains of ectomycorrhizal fungi, and the P and Pb concentrations in their mycelium. The results showed that under the application of P and Pb, the biomass production of strains ZC₁ and ZC₂ collected from mining area was significantly higher than that of strain GV collected from non-polluted site. At low P level (0.16 mmol·L^-1), the growth of these three ectomycorrhizal fungi was inhibited by the increasing dose of Pb; while at higher P levels (0.81and 2.42 mmol·L^-1), increasing dose of Pb inhibited the growth of GV significantly, but had little effects on ZC₁ and ZC₂. In all treatments, the Pb concentration in GV mycelia was significantly higher than that in the mycelia of ZC₁ and ZC₂. Under the same dose of Pb, the P concentration in GV mycelia was higher at low P level, but lower at higher P levels than that in ZC₁ and ZC₂ mycelia. The increase of Pb dose decreased the P concentration in ZC₁ and ZC₂ mycelia, but had little effect on that in GV mycelia. In comparing with GV, ZC₁ and ZC₂ had the characteristics of fast growth and low Pb and high P accumulation in their mycelia, indicating that the strains of ecomycorrhizal fungi collected from polluted area had a higher tolerance to environmental stress.

Key words: Fraxinus mandshurica, Phassus excrescens, Biology, Occurrence regularity