Abstract: In a pot experiment, axenic pomelo (Citrus grandis cv. Changshou Shatian You) seedlings were inoculated with arbuscular mycorrhizal (AM) fungi Gigaspora margarita, Glomus mosseae and Glomus versiforme, respectively, and their vegetative growth and mineral contents were studied. The results showed that the seedlings inoculated with test AM fungi could be infected effectively, and their shoot and root growth, especially fibrous root growth, was significantly improved, compared with the control. After inoculated with G. mosseae, the root length, lateral root number, and fibrous root number and length of the seedlings were increased by 22.7%, 35.7%, 160.8% and 1032%, respectively. The inoculation of these three AM fungi could significantly elevate the N, P, K, Ca, Mg, Zn, Cu and Mn contents in seedlings leaves. For example, the P content was increased by 46.8%, 88.7% and 32.3%, respectively, compared with the control. Among the three treatments, the seedlings inoculated with G. mosseae had the best vegetative growth, and the highest mycorrhizal dependence and N, P, K, Ca, Zn and Cu contents. The relative occurrence of AM fungal species decreased in the order of G. mosseae>G. margarita >G. versiforme, indicating that G. mosseae was most suitable for C. grandis seedlings inoculation.