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Morphological characteristics of resting eggs produced by different Brachionus calyciflorus strains

LIU Guiyun; XI Yilong   

  1. Provincial Key Laboratories of Conservation and Utilization for Important Biological Resource and Biotic Environment and Ecological Safety in Anhui, College of Life Science, Anhui Normal University, Wuhu 241000, China
  • Received:2005-06-01 Revised:2006-04-21 Online:2006-07-18 Published:2006-07-18

Abstract: In this paper, the Qingdao and Wuhu strains of Brachionus calyciflorus were cultured at 20 ℃, 25 ℃ and 30 ℃, and fed with 1.0×106 and 5.0×106 cells·ml-1 of Scenedesmus obliquus. The morphological study of their produced resting eggs showed that at the two food concentrations, the resting eggs produced at 20 ℃ were the largest in length, width and volume, while those produced at 25 ℃ and 30 ℃ were the smallest. The effects of strain on the morphological characteristics of resting eggs also depended on food concentration. When the food concentration was 1.0×106 cells·ml-1, the length, width and volume of the resting eggs produced by Wuhu strain and Qingdao strain were 156.00 μm, 99.95 μm and 12 269.11 μm3, and 145.13 μm, 91.97 μm and 10 498.19 μm3, respectively, while when the food concentration was 5.0×106 cells·ml-1, the corresponding values were 155.68 μm, 100.85 μm and 12 348.59 μm3, and 156.63 μm, 98.04 μm and 12 05420 μm3, respectively. For test strains, only Wuhu strain showed a marked curvilinear correlation in the length, width and volume of its resting eggs with temperature. At the same temperature, the volumes of the resting eggs produced by both Qingdao and Wuhu strains were increased with increasing food concentration, with the exception that the resting eggs produced by Wuhu strain at 30 ℃ decreased with increasing food concentration.

Key words: Stomatal conductance, Aneurolepidium chinense, Conductance simulation