Abstract: By the method of HPLC, and with indigo and indirubin as indicative constituents, this paper determined the effective constituents contents in indigowoad roots and leaves collected from different regions. The results showed that the total content of effective constituents in indigowoad root was the highest (16.27 mg·kg^-1) from Hanyin of Shaanxi Province, followed by Longde of Ningxia Autonomous Region (15.67 mg·kg^-1), Bozhou of Anhui Province (14.90 mg· kg^-1), and Linquan of Anhui Province and Jiamusi of Heilongjiang Province (13.97 mg·kg^-1), and that in indigowoad leaf had the same order, with the values being 698.32, 683.68, 680.11, 654.19 and 642.23 mg·kg^-1, respectively. The HPLC chromatograms of leaf samples collected from different regions were differed, not only in the test constituents, but also in the other constituents manifested as various peaks in the chromatogram. It was suggested that besides meteorological factors, the selection of geo-authentic region should be also involved in the cultivation of Isatis indigotica.

Key words: Soil ecology, Soil biota, Functional domains, Biodiversity, Global change