Abstract: The quantitative changes of anticancer ingredients such as taxol, cephalomannine, baccatin Ⅲ and 10-deacetylbaccatin Ⅲ in the branches and leaves of Taxus chinensis var. maireiduring one growth season were measured by HPLC. The results showed that the contents of these anticancer ingredients had an obvious change from April when the new branches began to germinate to November when the branches basically stopped growing. The contents of taxol and cephalomannine in the branches and leaves of T. chinensis had the same change pattern, with the peaks appeared in May, and those of Baccatin Ⅲ and 10-deacetylbaccatin Ⅲ had the same change trend, with the highest values appeared in September and April, respectively. There was a significant positive correlation between the contents of taxol and cephalomannine (P<0.05), and a negative correlation between the contents of cephalomannine and 10-deacetylbaccatin Ⅲ (P<0.05).