Abstract: Taking Trametes gallica mycelium pellets, Bacillus cereus, and their coimmobilized biomaterial as bio-adsorbents, this paper studied their Pb²⁺ adsorption under effects of different contact time, medium initial pH value and Pb²⁺ concentration, and bioadsorbent concentration, and compared the infrared spectra of the bio-adsorbents before and after Pb²⁺ absorption. The Pb²⁺ adsorption efficiency of the bio-adsorbents was the highest when the bioadsorbent concentration was 2 g·L^-1, initial pH was 5.0, initial Pb²⁺ concentration was 50 mg·L^-1, and contact time was 1 h, with the Pb2+
biosorption rate being 71.7% for the mycelium pellets of T. gallica, 91.0% for B. cereus, and 96.9% for the co-immobilized biomaterial. The infrared spectra of the bio-adsorbents were mainly consisted of the absorption zones of protein, carbohydrates, and sulphur and phosphorsbased groups, suggesting that hydroxyl, carboxyl, and sulphur and phosphatebased groups played important roles in the Pb²⁺ adsorption by the bio-adsorbents.