Abstract: By using acetylene inhibition method, this paper studied the soil denitrifying enzyme activity (DEA) and its affecting factors in the riparian zone with different land use types (cropland riparian, forested riparian, and grassy riparian zones) in Chongming Island, Shanghai of China. The riparian soil DEA was (0.69±0.11) - (134.93±33.72) μg N·kg^-1·h^-1, which differed obviously among different land types, with a decreasing trend of forested riparian zone > cropland riparian zone > grassy riparian zone. The soil DEA was significantly (P<0.05) higher in 0-10 cm in 10-30, 30-50, and 50-70 cm layers. There were significant positive relationships between soil DEA and soil TOC, TN, and NO₃^--N (P<0.01). Land use change mainly altered the soil natural structure and soil physical and chemical properties, decreased the accumulation of soil organic carbon, and affected the soil nitrogen transformation, and thus, inhibited the occurrence of riparian soil denitrification.