Abstract: Based on a copping system of “winter green manuredouble rice”, the 4× 4 twofactor test was used to study the effects of different nitrogen (N) application levels and winter green manure application on soil active organic carbon (AOC) and the C pool management index. The aim was to explore the ecological effects of winter green manure on soil improvement and determine the appropriate application levels of N fertilizer and winter green manure for improved rice yield. Results were as follows: 1) Compared with the control, the SOC and AOC contents increased by 22.2% and 26.7%, respectively, under the green manure only treatment, but the SOC contents decreased by 0.6%-3.4% under the single N fertilizer treatment. Compared with the control, the soil C pool management index increased by 24.55 and 15.17 under the green manure only and green manure plus N fertilizer treatments, respectively, and reduced by 2.59 under the single N fertilizer treatment. Compared with no fertilization, the average microbial biomass carbon (MBC)  increased by 54.0%, 95.2% and 14.3% under the green manure, green manure plus N fertilizer and single N fertilizer treatments, respectively. 2) The soil AOC content was significantly positively correlated with the C pool management index (P<0.01), and had a significant correlation with dissolved organic C and MBC (P<0.05). Rice yield was significantly positively correlated with AOC contents and the C pool management index, and the correlation coefficient was significantly greater than that with the total organic C. These results suggested that application of winter green manure at proper rates with inorganic fertilizer could increase SOC contents and the soil C pool management index, improve soil quality and fertility.