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Electrophysiological and behavioral responses of male Apamea apameoides (Draudt) (Lepidoptera: Noctuidae) to sex pheromone components.

ZHANG Ai-liang1, ZHOU Zhang-ting2, ZHANG Ya-bo3, ZHOU Zhi-feng2, SHEN Zhi-lian4, WANG Hao-jie3, SHU Jin-ping3   

  1. (1Anji Bureau of Forestry, Huzhou 313300, Zhejiang, China; 2Suichang Bureau of Forestry, Lishui 323300, Zhejiang, China; 3Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China; 4The People’s Government of Wukang Town, Huzhou 313300, Zhejiang, China)
  • Online:2014-10-18 Published:2014-10-18

Abstract: The sex pheromone gland extracts collected from calling females of Apamea apameoides (Lepidoptera: Noctuidae) were analyzed with GC-MS, the electrophysiological and behavioral responses of the male adults to serial dilutions of sex pheromone components and their synthetic blends were investigated with Ytube olfactometer in laboratory and in bamboo forest field. The results indicated that (Z)-11-hexadecenyl acetate and (Z)-11-hexadecen-1-ol were the functional components in the sex pheromone gland extracts. Electroantennogram (EAG) recordings showed that sex pheromone gland extracts, (Z)-11-hexadecenyl acetate, (Z)-11-hexadecen-1-ol and the mixture of (Z)-11-hexadecenyl acetate and (Z)-11-hexadecen-1-ol all could elicit strong EAG responses, and the average EAG values increased with the increasing concentration of the sex pheromone. The blends of (Z)-11-hexadecenyl acetate and (Z)-11-hexadecen-1-ol at the ratio of 57:43 elicited a higher EAG value than each singular component did. The results of behavioral assay by Y-tube olfactometer accorded with those of EAG responses on the whole, and the mixture of (Z)-11-hexadecenyl acetate and (Z)-11-hexadecen-1-ol at the ratio of 57:43 was more attractive than each component alone. In field tests with silicone rubber as pheromone dispensers (concentration=104 ng·uL-1), the average number of male adults captured per trap by the mixture was (48.5±6.7).