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Effects of salinealkali stress on electrical impedance spectroscopy parameters and ion contents in shoots of Ping’ou hybrid hazelnut.

ZHANG Li1,2, JIA Zhi-guo2, MA Qing-hua1, ZHANG Gang3, WANG Gui-xi1   

  1. (1Research Institute of Forestry, Chinese Academy of Forestry/ Key Laboratory of Forestry Silviculture of State Forestry Administration/State Key Laboratory of Tree Genetics and Breeding, Beijing 100091, China; 2Hebei North University, Zhangjiakou 075000, Hebei, China; 3Agricultural University of Hebei, Baoding 071000, Hebei, China)
  • Online:2014-11-18 Published:2014-11-18

Abstract: To study the adaptability to saltalkaline stress of Ping’ou hybrid hazelnut, ‘Liaozhen 3’ shoots which were treated with three types of stress neutral NaCl, alkaline Na2CO3, and mixed saltalkali, and the changes in electrical impedance spectroscopy (EIS) parameters and mineral ion contents were subsequently determined. The correlations between the EIS parameters and mineral ion contents were analyzed. The results showed that with the increasing level of NaCl, specific highfrequency resistance (r), specific lowfrequency resistance (r1), specific intracellular resistance (ri) and specific extracellular resistance (re) of shoots decreased firstly, then increased, and finally decreased again. However, these parameters increased gradually with the increasing level of Na2CO3, while r1 and re decreased slowly in the mixed saltalkali treatments. The Na+ contents of shoots increased significantly under the three saltalkali stresses with the order of NaCl stress > mixed saltalkali stress > Na2CO3 stress. Furthermore, Na2CO3 stress resulted in the decreases in the contents of three elements Zn, B and Ca. The significant negative correlation was found between the sum of five cations and four EIS parameters r1, re, relaxation time (τ), and distribution coefficient of relaxation time (Ψ). The shoots of ‘Liaozhen 3’ might be tolerant of Na2CO3 stress of 200 mmol·L-1, while they could be resistant to NaCl stress of 100-150 mmol·L-1.