Abstract: The variety of the contents of N, P, K and photo-biological characters in a bud and two leaves tea tree new shoots were determined to investigate the effects of different fertilization ways, i.e. single or combined N, P, K fertilizer. The regression equations of net photosynthetic rate (P_n) of tea tree in spring, summer and autumn were set up by stepwise regression analysis. The results showed that fertilization ways significantly affected the contents of N, P, K in new shoot. In the same fertilization ways, the contents of N, P in the new shoot was less in summer and autumn than in spring, but the content of K was in contrast. Combined application of N, P and K fertilizer improved P_n of the tea trees in spring. Single application of P fertilizer inhibited the P_n of the tea trees in summer and autumn, while single application of N fertilizer significantly improved it. In the same fertilization ways, the P_n of the tea trees in summer and autumn was higher than in spring. The stomatal conductance (g_s) and  transpiration rate (T_r) had positive correlations to P_n in summer and autumn. Regression and correlation analysis showed that the physiological factors affecting P_n of the tea trees varied with seasons, and the common factors were the C_i and the content of P. C_i had some subduction to P_n , and it was improved by the content of phosphorus.