Abstract: The effects of grafting on physiological characters of melon (Cucumis melo) seedlings under copper stress were investigated with Pumpkin Jingxinzhen No.3 as stock and oriental melon IVF09 as scion. The results showed that the physiological characters of melon seedlings were inhibited significantly under copper stress. Compared with selfrooted seedlings, the biomass, the contents of photosynthetic pigment, glucose and fructose, the photosynthetic parameters, the activities of sucrose phosphate synthase, neutral invertase  and acid invertase  in the leaves of the grafted seedlings were increased significantly. The uptake of nutrients was improved with the contents of K, P, Na increased and the content of Cu decreased. When the concentration of Cu²⁺ stress was 800μmol·L^-1, the contents of Cu in the leaves and roots of the grafted seedlings were decreased by 31.3% and 15.2%, respectively. Endogenous hormone balance of seedlings was improved by grafting. In the grafted seedlings, the content of IAA and peroxidase  activity were higher, whereas the contents of ABA, maleicdialdehyde, the activities of superoxide dismutase  and catalase  were lower than that in the control. It was concluded that the copper stress on the physiological characters of melon seedlings was relieved by grafting which improved the resistance of the grafted seedlings.