Abstract: A packed soil column experiment was conducted to investigate the effect of different fertilization practices on phosphorus (P) accumulation and leaching potential in a vegetable greenhouse soil with different fertility levels. The results showed that the leaching loss of total P in the leachates elevated with the increment of leaching time while the accumulative leaching loss of total P was relatively low, indicating P was mainly accumulated in the soil instead of in the leachate. At the end of the leaching experiment, soil fertility and fertilization treatment affected the content of total phosphorus and OlsenP significantly. Compared with the lowlevelfertility soil, the contents of total P and OlsenP increased by 14.3% and 12.2% in the mediumlevelfertility soil, 33.3% and 37.7% in the highlevelfertility soil. Total P in the combined application of poultry manure and chemical fertilizer (M+NPK)  was elevated by 5.7% and 4.3%, compared with the NPK and M treatment. Compared  with NPK treatment, OlsenP in M and M+NPK treatments augmented by 13.0% and 3.1%, respectively. Soil total P and OlsenP mainly accumulated in the 0-10 cm and 10-20 cm soil layers, and much less in the 20-40 cm soil layer.