Abstract: Based on the GIS software platform, referring to the China ‘Terrestrial ecosystem services per unit area value’, and by using transition matrix, Costanza evaluation formula, and sensitivity analysis, this paper studied the change characteristics of land use and ecosystem services value in Ji’nan City in 1989-2009. During the study period, the builtup area in the City increased by 99.65 km², while agriculture land and green space reduced by 103.21 km², 90.4% of which was taken by the built-up land. The total ecosystem services value decreased from 256.22×10⁶￥ to 214.16×10⁶ ￥, with a decrement of 42.06×10⁶ ￥, mainly due to the decrease in the areas of agriculture land and green space. For the sustainable development of the population, resources, and environment in Ji’nan City, future urban planning should pay more attention on the natural resources protection, reasonable planning of land use structure, and maintenance of ecosystem stability and balance.