Abstract: Two winter wheat cultivars with different drought tolerance were selected to investigate the effects of source-sink regulation on the vegetative organs water soluble carbohydrates (WSC) content and 1000-grain mass (TGM) of wheat under drought stress (DS) and well watered (WW) conditions. Sink-cutting increased the WSC content of different vegetative organs significantly, and promoted the relative transportation of the WSC positively; while sourcecutting caused opposite responses. The effects of sourcesink regulation on the WSC content and its relative transportation amount (TA) and transportation rate (TR) were significantly higher under DS and sinkcutting than under WW and source-cutting, for droughtresistant cultivar (Longjian 19) than for drought-sensitive Q9086, and for peduncle internode and PedI than for penultimate internode and PenI. Under source-cutting, the superior organs of Longjian 19 in the TR of total WSC were sheath, PedI, and PenI, which also contributed to the fructan TR of the two cultivars, while those of Q9086 were the PenI and the third internode from top (ThiI). Source-cutting decreased the TGM of Longjian 19 and Q9086 significantly, with the decrement being 27.3% and 31.7% under DS and 25.3% and 12.1% under WW, respectively. The correlation coefficients of the WSC content and its TA and TR and the TGM were significantly higher under sink-cutting than under source-cutting, and also, under DS than under WW. There existed a higher correlation coefficient (r²> 0.900) of the TGM and the total WSC and fructan contents in different vegetative organs. The vegetative organs with closer correlation between their WSC content and its TA and TR and the TGM were mainly sheath and PedI. Under DS, the traits associated with the total WSC content had a higher correlation with TGM; under WW, the traits associated with sucrose and glucan contents generally showed a higher correlation with TGM. It was suggested that the effects of sourcesink regulation on the WSC content of vegetative organs and the TGM were significantly affected by soil water environment, wheat genotype, and vegetative organs location.