Effects of hydrogen sulfide on mitochondrial function in  sweet cherry stigma and ovary under low temperature stress

doi:10.13287/j.1001-9332.202004.030

Abstract

Abstract: To investigate the effects of H₂S on mitochondrial functions under low temperature stress, we analyzed the effects of 0.05 mmol·L^-1NaHS and 15 μmmol·L^-1HT (hypotaurine and H₂S scavenger) on mitochondria antioxidant enzyme activities and mitochondrial permeability transition pore, mitochondrial membrane fluidity, mitochondrial membrane potential, Cyt c/a ratio and H⁺-ATPase activity in sweet cherry stigma and ovary with sweet cherry variety Zaodaguo under -2 ℃ low temperature stress. The results showed that low temperature stress increased the concentrations of mitochondrial H₂O₂ and MDA, enhanced the mitochondrial membrane permeability, but decreased the mitochondrial membrane fluidity, membrane potential, Cyt c/a and H⁺-ATPase acti-vity. Application of NaHS at 0.05 mmol·L^-1 could effectively reduce the concentrations of H₂O₂ and MDA, and keep higher activities of SOD, POD and CAT of mitochondrial for longer time. Furthermore, application of 0.05 mmol·L^-1NaHS could decrease mitochondrial membrane permeability while increase mitochondrial membrane fluidity, membrane potential, Cyt c/a and H⁺-ATPase activity in stigma and ovary under low temperature stress. The effects of NaHS were completely offset by HT addition. The results suggested that exogenous H₂S could alleviate the oxidative damage on stigma and ovary stress through decreasing H₂O₂ accumulation, regulating mitochondria antioxidant system, increasing H⁺-ATPase activity, and mitigating mitochondria function under low temperature.

WEI Guo-qin, TAO Ji-han, FU Quan-juan, HOU Sen, YANG Xing-hua, SUI Shu-guang, YU Xian-mei, SUN Yu-gang. Effects of hydrogen sulfide on mitochondrial function in sweet cherry stigma and ovary under low temperature stress[J]. Chinese Journal of Applied Ecology, 2020, 31(4): 1121-1129.

References 43

[1]	Ma HY, Lv DG, Liu GC. Mitochondrial response in the apical and lateral flower buds of the Hanfu apple to cold stress during the dormancy stage. Acta Ecologica Sinica, 2013, 33: 52-58
[2]	生利霞, 冯立国, 束怀瑞, 等. 低氧胁迫下外源硝态氮对樱桃根系功能及氮代谢相关酶活性的影响. 园艺学报, 2009, 36(11): 1575-1580 [Sheng L-X, Feng L-G, Shu H-R. Effects of nitrogen on the activity of antioxidant enzymes and functions of mitochondria in cherry rootstock roots under hypoxia stress. Acta Horticulturae Sinica, 2009, 36(11): 1575-1580]
[3]	张召, 梁元存, 王利,等. 钙对酸化处理平邑甜茶根系抗氧化酶活性及线粒体功能的影响. 林业科学, 2012, 48(8): 87-93 [Zhang S, Liang Y-C, Wang L, et al. Effect of calcium on the functions of antioxidant systems and mitochondria in Malus hupehensis var. pingyiensis roots under acid treatment. Scientia Silvae Sinicae, 2012, 48(8): 87-93]
[4]	Zhan J, Li W, He HY, et al. Mitochondrial alterations during Al-induced PCD in peanut root tips. Plant Physio-logy and Biochemistry, 2014, 75: 105-113
[5]	潘雄波, 向丽霞, 胡晓辉, 等. 外源亚精胺对盐碱胁迫下番茄幼苗根系线粒体功能的影响. 应用生态学报, 2016, 27(2): 491-498 [Pan X-B, Xiang L-X, Hu X-H, et al. Effects of exogenous spermidine on mitochondrial function of tomato seedling roots under salinity-alkalinity stress. Chinese Journal of Applied Ecology, 2016, 27(2): 491-498]
[6]	李顺, 景举伟, 严金平, 等. 气体信号分子H2S在植物中的研究进展. 植物生理学报, 2015, 51(5): 579-585 [Li S, Jing J-W, Yan J-P, et al. Research progress in the gasotransmitter hydrogen sulde in plants. Journal of Plant Physiology, 2015, 51(5): 579-585]
[7]	Bharwana SA, Ali S, Farooq MA, et al. Hydrogen sulfide ameliorates lead-induced morphological, photosynthetic, oxidative damages and biochemical changes in cotton. Environmental Science and Pollution Research, 2014, 21: 717-731
[8]	Shan C, Liu H, Zhao L, et al. Effects of exogenous hydrogen sulfide on the redox states of ascorbate and glutathione in maize leaves under salt stress. Biologia Plantarum, 2014, 58: 169-173
[9]	Jin ZP, Shen JJ, Qiao ZJ, et al. Hydrogen sulfide improves drought resistance in Arabidopsis thaliana. Biochemical and Biophysical Research Communications, 2011, 414: 481-486
[10]	Chen Z, Chen M, Jiang M. Hydrogen sulfide alleviates mercury toxicity by sequestering it in roots or regulating reactive oxygen species productions in rice seedlings. Plant Physiology and Biochemistry, 2017, 111: 179-192
[11]	Zhang H, Ye YK, Wang SH, et al. Hydrogen sulfide counteracts chlorophyll loss in sweet potato seedling leaves and alleviates oxidative damage against osmotic stress. Journal of Plant Growth Regulation, 2009, 58: 243-250
[12]	Li ZG, Gong M, Xie H, et al. Hydrogen sulfide donor sodium hydrosulfide-induced heat tolerance in tobacco (Nicotiana tabacum L.) suspension cultured cells and involvement of Ca2+ and calmodulin. Plant Science, 2012, 185/186: 185-189
[13]	Liu X, Chen J, Wang GH, et al. Hydrogen sulfide alleviates zinc toxicity by reducing zinc uptake and regulating genes expression of antioxidative enzymes and metallothioneins in roots of the cadmium/zinc hyper-accumulator Solanum nigrum L. Plant and Soil, 2016, 400: 177-192
[14]	Tian BH, Qiao ZJ, Zhang LP, et al. Hydrogensulfide and proline cooperate to alleviate cadmium stress infoxtail millet seedlings. Plant Physiology and Biochemistry, 2016, 109: 293-299
[15]	Chen J, Wang WH, Wu FH, et al. Hydrogen sulfide alleviates aluminum toxicity in barley seedlings. Plant and Soil, 2013, 362: 301-318
[16]	Cheng W, Zhang L, Jiao CJ, et al. Hydrogen sulfide alleviates hypoxia-induced root tip death in Pisum sativum. Plant Physiology and Biochemistry, 2013, 70: 278-286
[17]	Wang YQ, Li L, Cui WT, et al. Hydrogen sulfide enhances alfalfa (Medicago sativa) tolerance against salinity during seed germination by nitric oxide pathway. Plant and Soil, 2012, 351: 107-119
[18]	魏国芹, 杨洪强, 付全娟, 等. H2S对低温胁迫下甜樱桃柱头和子房AsA-GSH循环的响应. 核农学报, 2017, 31(6): 1217-1225 [Wei G-Q, Yang H-Q, Fu Q-J, et al. Effects of H2S on ascorbate-glutathione cycle in sweet cherry stigma and ovary under low temperature stress. Journal of Nuclear Agricultural Sciences, 2017, 31(6): 1217-1225]
[19]	杨玖英, 谭艳平, 夏春皎, 等. 红莲型细胞质雄性不育性与线粒体渗透性转换. 武汉植物学研究, 2004, 22(5): 385-390 [Yang J-Y, Tan Y-P, Xia C-J, et al. Honglian cytoplasmic male sterility in relation to its mitochondrial permeability transition. Wuhan Botanical Research, 2004, 22(5): 385-390]
[20]	Panda SK, Yamamoto Y, Kondo H, et al. Mitochondrial alterations related to programmed cell death in tobacco cells under aluminium stress. Comptes Rendus Biologies, 2008, 331: 597-610
[21]	Gioniannopolitis CN, Ries SK. Purification and quantitative relationship with water-soluble protein in seedling. Plant Physiology, 1977, 59: 315-318
[22]	Kochba J, Lavee S, Spiegel-Roy P. Differences in peroxidase activity and isoenzymes in embryogenic and nonembryogenic ‘Shamout’orange ovular callus lines. Plant and Cell Physiology, 1977, 18: 463-467
[23]	Dhindsa RS, Plumb-Dhindsa P, Thorpe TA. Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 1982, 32: 93-101
[24]	邵小杰, 杨洪强, 乔海涛, 等. 氯化镉胁迫下葡萄根、叶内源一氧化氮和活性氧的生成. 应用生态学报, 2009, 20(6): 1390-1394 [Shao X-J, Yang H-Q, Qiao H-T, et al. Endogenous NO and ROS generation in grape roots and leaves under CaCl2 stress. Chinese Journal of Applied Ecology, 2009, 20(6): 1390-1394]
[25]	苏宏, 李丽杰, 马怀宇. 等. 变温条件下钙对山定子根系线粒体功能的影响. 中国农业科学, 2014, 47(19): 3866-3873 [Su H, Li L-J, Ma H-Y, et al. Effects of calcium on mitochondrial function in Malus baccata Borkh. roots under changing temperature. Scientia Agricultura Sinica, 2014, 47(19): 3866-3873]
[26]	马怀宇, 杨洪强. 外源H2O2对湖北海棠根系线粒体膜透性和细胞核DNA的影响. 植物生理与分子生物学学报, 2006, 32(5): 551-556 [Ma H-Y, Yang H-Q. The effect of exogenous H2O2 on mitochondrial membrane permeability and cell nuclear DNA in roots of Malus hupehensis. Journal of Plant Physiology and Molecular Biology, 2006, 32(5): 551-556]
[27]	陆晓民, 孙锦, 何立中, 等. 油菜素内酯对低氧胁迫黄瓜幼苗根系线粒体抗氧化系统及其细胞超微结构的影响. 园艺学报, 2012, 39(5): 888-896 [Lu X-M, Sun J, He L-Z, et al. Effects of brassinolide on the mitochondria antioxidant system and cellular ultrastructure of cucumber seedling roots under hypoxic stress. Acta Horticulturae Sinica, 2012, 39(5): 888-896]
[28]	Zoratti M, Szabo I. The mitochondrial permeability transition. Biochimicaet Biophysica Acta, 1995, 1241: 139-176
[29]	陈靠山, 刘世名. 用ANS研究脱落酸提高植物线粒体膜的流动性. 生物物理学报, 2000, 16(2): 237-242 [Chen K-S, Liu S-M. Research on increase of membrane fluidty of plant mitochondria by abscisic acid with ANS. Acta Biophysica Sinica, 2000, 16(2): 237-242]
[30]	Qian P, Sun R, Ali B, et al. Effects of hydrogen sulfide on growth, antioxidative capacity, and ultrastructural changes in oilseed rape seedlings under aluminum toxicity. Plant Growth Regulation, 2014, 33: 526-538
[31]	Wei GQ, Zhang WW, Cao H, et al. Effects hydrogen sulfide on the antioxidant system and membrane stability in mitochondria of Malus hupehensis under NaCl stress. Biologia Plantarum, 2019, 63: 228-236
[32]	Jang S, Javadov S. Association between ROS production, swelling and the respirasome integrity in cardiac mitochondria. Archives of Biochemistry and Biophysics, 2017, 630: 1-8
[33]	Szabo I, Zoratti M. Mitochondrial channels: Ion fluxes and more. Physiological Reviews, 2014, 94: 519-608
[34]	Zorov DB, Juhaszova M, Sollott SJ. Mitochondrial reactive oxygen species (ROS) and ROS-induced ROS release. Physiological Reviews, 2014, 94: 909-950
[35]	Ichas F, Mazat JP. From calcium signaling to cell death: Two conformation for the mitochondrial permeability transition pore. Switching from low- to high-conductance state. Biochimica et Biophysica Acta, 1998, 1366: 33-50
[36]	Li L, Peter R, Philip KM. Hydrogen sulfide and cell signaling. Annual Review of Pharmacology and Toxicology, 2011, 51: 169-187
[37]	王诗军, 李钰婷, 孙浩林, 等. 气体信号分子硫化氢调节细胞功能的作用与机制. 中国组织工程研究, 2014, 18(20): 3275-3278 [Wang S-J, Li Y-T, Sun H-L, et al. Effect and mechanism of hydrogen sulfide in regulating cellular functions. Chinese Journal of Tissue Engineering Research, 2014, 18(20): 3275-3278]
[38]	Halestrap AP, Gillespie JP, O’Toole A, et al. Mito-chondria and cell death: A pore way to die?// Bryant JA, Hughes SG, Garland JM, eds. Programmed Cell Death in Animals and Plants. Oxford: BIOS Scientific Publishers, 2000: 149-162
[39]	Krishnan N, Fu CX, Pappin DJ, et al. H2S-induced sulfhydration of the phosphatase PTP1B and its role in the endoplasmic reticulum stress response. Science Signaling, 2011, 4: ra86
[40]	Sen N, Paul BD, Gadalla MM, et al. Hydrogen sulfide-linked sulfhydration of NF-κB mediates its antiapoptotic actions. Molecular Cell, 2012, 45: 13-24
[41]	Yang GD, Zhao KX, Ju YJ, et al. Hydrogen sulfide protects against cellular senescence via S-sulfhydration of Keap1 and activation of Nrf2. Antioxid Redox Signal, 2013, 18: 1906-1919
[42]	Gade AR, Kang M, Akbarali HI. Hydrogen sulfide as an allosteric modulator of ATP-sensitive potassium channels in colonic inflammation. Molecular Pharmacology, 2013, 83: 294-306
[43]	刘志强, 裴雁曦, 方慧慧, 等. H2S巯基化修饰蛋白质调节谷子响应逆境胁迫. 中国生物化学与分子生物学报, 2015, 31(10): 1085-1091 [Liu Z-Q, Pei Y-X, Fang H-H, et al. H2S regulates foxtail millet responsing to stress by protein S-sulfhydration. Chinese Journal of Biochemistry and Molecular Biology, 2015, 31(10): 1085-1091]

Effects of hydrogen sulfide on mitochondrial function in sweet cherry stigma and ovary under low temperature stress

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