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Effects of exogenous AsA and GSH on the growth of Dianthus chinensis seedlings exposed to Cd.

DING Ji-jun1,2, LIU Shi-liang1, PAN Yuan-zhi1, LI Li3   

  1. (1College of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, China; 2Three Gorges Industrial Co. Ltd., Yichang 443002, Hubei, China; 3College of Forestry, Southwest Forestry University, Kunming 650224, China)
  • Online:2014-02-18 Published:2014-02-18

Abstract: A pot experiment was carried out under greenhouse condition to investigate the effects of different concentrations (0, 20, 40, 60, 80 and 100 mg·L-1) of exogenous AsA, GSH on Dianthus chinensis seedlings which were stressed by 50 mg·kg-1 Cd in the soil. The results indicated that 50 mg·kg-1 of Cd significantly inhibited the growth of D. chinensis seedlings. An appropriate concentration of exogenous AsA significantly improved the biomass, plant height, tiller number, GAT and APX activities, and AsA and GSH contents. However, with the increase of exogenous AsA concentration, the ameliorating effect decreased and prooxidant effect occurred. Exogenous GSH could replenish the nonenzymatic antioxidants of D. chinensis seedlings, but the changes of antioxidant enzyme activities were relatively slight. The main mechanisms of GSH to alleviate Cd toxicity might be promoting root PCs synthesis, thereby reducing the Cd concentration in the seedlings. Both 35-45 mg·L-1 exogenous AsA and 55-65 mg·L-1 exogenous GSH could alleviate the Cd toxicity on D. chinensis seedlings, and the former was superior to the latter.