Abstract: Five-year-old ‘Fuji’3/M26/M. hupehensis Rehd. seedlings were treated by ¹⁵N tracer to study the effects of fertilization depth (0, 20 and 40 cm) on ¹⁵Nurea absorption, distribution, utilization and loss in soil. The results showed that the plant leaf area, chlorophyll content and total N of apple leaves in 20 cm treatment were obviously higher than 0 cm and 40 cm treatments. The ¹⁵N derived from fertilizer (Ndff) in different organs of apple plant under different depths were significantly different, and the Ndff was the highest in roots at the fullbloom stage, and then in perennial branches. During the shoot rapidgrowing and flower bud differentiation stage, the Ndff of new organs higher than that of the storage organs, and the Ndff of different organs were high level at fruit rapidexpanding stage, and the Ndff of fruit was the highest. The distribution ratio of ¹⁵N at fruit maturity stage was significantly different under fertilization depths, and that of the vegetative and reproductive organs of 20 cm treatment were obviously higher than 0 cm and 40 cm treatments, but that of the storage organs of 20 cm treatment was lower than 0 cm and 40 cm treatments. At fruit maturity stage, ¹⁵N utilization rate of apple plant of 20 cm treatment was 24.0%, which was obviously higher than 0 cm (14.1%) and 40 cm (7.6%) treatments, and ¹⁵N loss rate was 54.0%, which was obviously lower than 0 cm (67.8%) and 40 cm (63.5%) treatments. With the increase of fertilization depths, the N residue in soil increased sharply.