Damage of deltamethrin on tissue and cell DNA in hepatopancreas of Pagrosomus major

doi:10.13287/j.1001-9332.201706.014

Abstract

Abstract: In order to study the toxic effect of deltamethrin on the hepatopancreas of Pagrosomus major with sub-acute exposure, Pagrosomus major were divided into 5 groups with different doses of semi-static exposure. The damage of the hepatopancreas tissue of Pagrosomus major was analyzed by microscopy observation and the DNA damage of the cells was analyzed by comet assay technique after 25 days exposure. The results showed that different degrees of congestion, nuclear enrichment and cell necrosis appeared in hepatopancreas of 0.025, 0.125, 0.250, 0.375 μg·L^-1 exposure concentrations. The higher the exposure concentration, the variations of tissue and cell were more significant. DNA damages in the hepatopancreas cells of all exposed group were observed. The co-met rate, percentage of tail DNA and the olive moment were significantly different from the control group (P<0.05). Linear regression analysis showed that the exposure concentration was significantly positively correlated with the tailing rate and the tail length (P<0.01). The indexes were significantly related to the concentration of deltamethrin exposure with the coefficient of determination between 0.909-0.996. It was suggested that deltamethrin could cause various degree of damage to the tissue and cellular DNAs in the hepatopancreas of Pagrosomus major in a linear responsive manner.

ZHENG Hui-dong. Damage of deltamethrin on tissue and cell DNA in hepatopancreas of Pagrosomus major[J]. Chinese Journal of Applied Ecology, 2017, 28(6): 2033-2039.

References

[1] Chen H-H (陈辉辉), Qin J-H (覃剑晖), Liu H-C (刘海超), et al. Acute toxicity of representative heavy metals, polycyclic aromatic hydrocarbons (PAHs) and pyrethroid pesticide to Tanichthys albonubes. Journal of Huazhong Agricultural University (华中农业大学学报), 2011, 30(4): 511-515 (in Chinese)
[2] Viran R, Unlü EF, Polat H, et al. Investigation of acute toxicity of deltamethrin on guppies (Poecilia reticulata). Ecotoxicology and Environmental Safety, 2003, 55: 82-85
[3] Zhao Y-Q (赵玉琴), Li L-N (李丽娜), Li J-H (李建华). Study of acute and joint toxicity of common pyrethroid and organophosphate pesticides to fish. Environmental Pollution and Control (环境污染与防治), 2008, 30(11): 53-57 (in Chinese)
[4] Suvetha L, Saravanan M, Hur JH, et al. Responses of the Indian major carp Labeo rohita to deltamethrin at acute and sublethal concentrations. Toxicological and Environmental Chemistry, 2015, 97: 186-199
[5] Hamed RR, Maharem TM, Guinidi RAM. Glutathione and its related enzymes in the Nile fish. Fish Physiology and Biochemistry, 2004, 30: 189-199
[6] Jin X-M (靳晓敏),Wu Y (吴垠), Lu G (卢刚), et al. Effects of two pyrethrin pesticides on serum lysozyme and glutamic pyruvic transaminase in common carp. Fisheries Science (水产科学), 2006, 25(8): 383-386 (in Chinese)
[7] Suvetha L, Ramesh M, Saravanan M. Influence of cypermethrin toxicity on ionic regulation and gill Na⁺/K⁺-ATPase activity of a freshwater teleost fish Cyprinus carpio. Environmental Toxicology and Pharmacology, 2010, 29: 44-49
[8] Wei H (魏华), Wu N (吴楠), Shen H (沈竑), et al. Oxidative stress of deltamethrin to the liver of crayfish (Procambarus clarkii). Journal of Fisheries of China (水产学报), 2010, 34(5): 733-739 (in Chinese)
[9] Wu W (吴伟), Chen J-Z (陈家长), Ma X-Y (马晓燕), et al. Accumulation of deltamethrin in the tissue of Tilipia and the influence on activity of Glutathione-S-transferase. Journal of Agro-Environment Science (农业环境科学学报), 2006, 25(5): 1176-1181 (in Chinese)
[10] Prusty AK, Kohli MPS, Sahu NP, et al. Effect of short term exposure of fenvalerate on biochemical and haematological responses in Labeo rohita (Hamilton) fingerlings. Pesticide Biochemistry and Physiology, 2011, 100: 124-129
[11] Wang R-L (王瑞龙), Chen Y-M (陈玉明), Xu J (徐军), et al. Effects of cypermethrin on superoxide dismutase(SOD) activities in liver and gill tissues of Tanichthys albonubes. Ecology and Environment (生态环境), 2007, 16(3): 790-793 (in Chinese)
[12] Wu N (吴楠), Wei H (魏华), Shen H (沈竑), et al. The toxic research of deltamethrin on the muscle tissue of the red swamp crayfish(Procambarus clarkia). Journal of Fisheries of China (水产学报), 2015, 39(9): 1412-1421 (in Chinese)
[13] Yan H-Y (闫海燕), Zeng L-B (曾令兵), Luo Y-L (罗宇良), et al. Effect of fenvalerate on activity of SOD, content of MDA and histomorphology of liver and kidney of carp. Journal of Huazhong Agricultural University (华中农业大学学报), 2012, 31(4): 499-505 (in Chinese)
[14] Xie W-P (谢文平), Zhu X-P (朱新平), Chen K-C (陈昆慈), et al. Toxic effects of cypermethrin on Na⁺/K⁺-ATP activity and ultramicrostructure of gill and liver tissue in grass carp. Journal of Fishery Sciences of China (中国水产科学), 2009, 16(1): 120-125 (in Chinese)
[15] Huangfu J-Q (皇甫加清), Zhang Y-G (张耀光), Zhou C-J (周传江), et al. Effect of cypermethrin on histology of four tissues of Ctenopharyngodon idellus. Freshwater Fisheries (淡水渔业), 2011, 41(1): 53-57 (in Chinese)
[16] Velmurugan B, Selvanayagam M, Cengiz EI, et al. The effects of fenvalerate on different tissues of freshwater fish Cirrhinus mrigala. Journal of Environmental Science and Health Part B, 2007, 42: 157-163
[17] Chen Y-Q (陈仪取), Feng T (冯涛), Guo X-Q (郭祥群). Effects of three kinds of pesticides on antioxidant enzymes and acetylcholinesterase activities of Lateolabrax japonicus. Journal of Xiamen University (Natural Science)(厦门大学学报:自然科学版), 2004, 43(6): 828-832 (in Chinese)
[18] Lan X (兰欣). In vitro cytotoxicity of the pyrethroid insecticide fenvalerate to the flounder (Paralichthys olivaceus) gill cells. Journal of Heze University (荷泽学院学报), 2010, 32(5): 74-77 (in Chinese)
[19] Zhao L-N (赵李娜). Study on the Residues and Risk Assessment of 7 Pyrethroid Pesticides in the Water Environment of the Pearl River Delta. Master Thesis. Shanghai: Shanghai Ocean University, 2014 (in Chinese)
[20] Song Z-R (宋振荣). Pathology of Aquatic Animal. Xiamen: Xiamen University Press (in Chinese)
[21] Zhao F (赵飞), Wang B (王摆), Zhang X-N (张晓娜), et al. DNA damage induced by monoerotophos pesticide and the underlying mechanism in hepatic cells of goldfish (Carassius auratus). China Environmental Science (中国环境科学), 2015, 35(5): 1563-1569 (in Chinese)
[22] Feng T (冯涛), Wei F-Q (魏凤琴), Ouyang G-L (欧阳高亮), et al. Effect of BAP exposure on ultrastructures of hepatic cells of Boleophthalmus pectinirostris. Chinese Journal of Applied Ecology (应用生态学报), 2003, 14(10): 1780-1782 (in Chinese)
[23] Liu W-C (刘伟成), Li M-Y (李明云), Huang F-Y (黄福勇), et al. Effects of cadmium stress on xanthine oxidase and antioxidant enzyme activities in Boleophthalmus pectinirostris liver. Chinese Journal of Applied Ecology (应用生态学报), 2006, 17(7): 1310-1314 (in Chinese)
[24] Polat H, Erkoç FU, Viran R, et al. Investigation of acute toxicity of beta-cypermethrin on guppies Poccilia reticulate. Chemosphere, 2002, 49: 39-44
[25] Chen X-R (陈秀荣), Luo Y-L (罗宇良), Zeng L-B (曾令兵), et al. Effect of fenvalerat on activtity of hepatic CYP3A in Grass Carp. Journal of Huazhong Agricultural University (华中农业大学学报), 2010, 29(6): 768-771 (in Chinese)
[26] Bruslè J, Anadon GG. Fish morphology: The structure and function of fish liver// Munshi JSD, Dutta HM, eds. Fish morphology. New York: Science Publishers, 1996: 77-93
[27] Ostling O, Johanson KJ. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochemical and Biophysical Research Communications, 1984, 123: 291-298
[28] Lee RF, Steinert S. Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals. Mutation Research, 2003, 544: 43-64
[29] Jin YX, Zheng SS, Pu Y, et al. Cypermethrin has the potential to induce hepatic oxidative stress, DNA damage and apoptosis in adult zebrafish (Danio rerio). Chemosphere, 2011, 82: 398-404
[30] Liu B (刘波), Xu Y-B (许贻斌), Zheng H-D (郑惠东), et al. The analysis on digestive glands cells of Ruditapes philippinarum exposed to fenvalerate with comet assay method. Journal of Fisheries of China (水产学报), 2014, 38(5): 671-676 (in Chinese)
[31] Liu B (刘波), Chen Y-F (陈宇峰), Zheng H-D (郑惠东), et al. The research on DNA damage in hepa-topancrea cells of Marsupenaeus japonicas exposed to ethofenprox. Marine Sciences (海洋科学), 2014, 38(11): 29-34 (in Chinese)
[32] Song W-H (宋文华), Pei Y-T (裴亚托), Liu Q (刘强), et al. Investigation on DNA damage induced by the photolysis product of diflubenzuron in mice liver using single cell gel electrophoresis. Journal of Agro-Environment Science (农业环境科学学报), 2007, 26(4): 1482-1486 (in Chinese)