Abstract: A two-year (2013-2014) field experiment was conducted to explore the effect of cropping systems (corn monoculture, soybean monoculture and corn-soybean intercropping) and nitrogen application rate (0, 150 kg·hm^-2) on soil meso- and micro-invertebrate community composition and structure by using Tullgren in red soil. The results showed that a total of 7860 individuals were found during the two years, which belonged to 29 orders, 10 classes and 3 phyla. The dominant groups were Acarina and Collembola. Intercropping and nitrogen application both increased the average density of soil fauna at jointing stage of corn in 2013. The number of groups of soil fauna was obvious higher in 2014 than that in 2013. Meanwhile, nitrogen application could increase the average density of soil fauna in 2014. From the results of two-year multi-variance analyses on soil fauna, cropping pattern and corn growth stage had no significant impact on soil meso- and micro-invertebrate community. But in 2014, nitrogen application significantly affected the soil fauna group, indexes of Simpson and density-group (DG); the interaction between cropping pattern and corn growth stage obviously affected soil fauna average density, group number, DG and abundance; the interaction between nitrogen application rate and corn growth stage obviously affected indexes of Simpson, DG and Shannon. From the results of detrended correspondence analysis (DCA), cropping pattern and nitrogen application rate had no obvious impact on soil meso- and micro-invertebrate community in red soil. The dynamic of community structure was related with corn growth stages. In conclusion, corn-soybean intercropping with reasonable nitrogen application could improve soil meso- and micro-invertebrate average density and group, and sustain a good diversity of soil fauna in red soil.