Evaluation of cold resistance of four wild Carex speices

doi:10.13287/j.1001-9332.201701.035

Abstract

Abstract: Four wild Carex speices (C. rigescens, C. lancifolia, C. leucochlora and C. humilis) collected from Mount Taishan were used as experimental materials. The adaption across the winter and physiological characteristics resistant to cold stress were investigated, semi-lethal temperature (LT₅₀) was calculated and fuzzy subordination method was used to generally evaluate the Carex resistant to cold stress. The results showed that 4 Carex species all survived in the winter safely and restored well to grow in the following spring. The green period of the species was between 260 d and 310 d, and percentage of the withered leaves was between 12% and 95%, the range of LT₅₀ was from -18.65 ℃ to -11.74 ℃. With intensifying cold stress, the contents of MDA, proline (Pro) and soluble protein increased at first and then decreased, while the soluble sugar content increased with the treatment time. C. rigescens with poor cold tolerance showed the early accumulation of MDA, Pro and soluble sugar. The value of soluble protein peaked at the late stage of low tempe-rature stress, and the Carex with stronger cold-resistance showed the smaller value. The SOD activity in the leaves of C. lancifolia was higher than that of the other three species in the beginning of treatments. The cold resistance of four Carex species was in order of C. lancifolia>C. leucochlora>C. humilis>C. rigescens.

YE Yan-ran, WANG Wen-li, ZHENG Cheng-shu, FU De-jing, LIU Hui-wen. Evaluation of cold resistance of four wild Carex speices[J]. Chinese Journal of Applied Ecology, 2017, 28(1): 89-95.

References

[1] Li F-Z (李法增). Shandong Plants Essence. Beijing: Science Press, 2004 (in Chinese)
[2] Ma W-L (马万里), Han L-B (韩烈保), Luo J-C (罗菊春). New sources of lawn plant: Genus Carex L. Practaculture Science (草业科学), 2001, 18(2): 43-45 (in Chinese)
[3] Wang J-Q (王俊强), Lv H-G (吕会刚), Fang W (方唯), et al. The research and application of Carex germplasm resources. Beijing Landscape Architecture (北京园林), 2006, 22(2): 36-38 (in Chinese)
[4] Reznicek AA. Evolution in sedges (Carex, Cyperaceae). Canadian Journal of Botany, 1990, 68: 1409-1432
[5] Xue H (薛红), Sha W (沙伟), Ni H-W (倪红伟). The research work of the Carex spp. Journal of Qiqihar University (齐齐哈尔大学学报), 2005, 21(4): 81-86 (in Chinese)
[6] Marlena L, Pawel O. Effect of mother plant age on germination and size of seeds and seedlings in the perennial sedge Carex secalina (Cyperaceae). Flora, 2011, 206: 158-163
[7] Ning H, Wang WL, Zheng CS, et al. Genetic diversity analysis of sedges (Carex spp.) in Shandong, China based on inter-simple sequence repeat. Biochemical Systematics and Ecology, 2014, 56: 158-164
[8] Teng Z-H (滕中华), Han F (韩发), Shi S-B (师生波), et al. Seasonal changes of substance related with cold resistance in Carex atrofusca in Qinghai Tibetan Plateau. Grassland of China (中国草地), 2003, 25(4): 37-41 (in Chinese)
[9] Xiao Y-F (萧运峰), Sun F-Z (孙发政), Gao J (高洁). Wild lawn plant: The research of Carex leucochlora. Journal of Sichuan Grassland (四川草地学报), 1995(2): 29-32 (in Chinese)
[10] Li X-L (李秀玲), Liu K-Q (刘开强), Yang Z-M (杨志民), et al. Effect of drought stress on withered leaf rate and physiological indices of four ornamental grasses. Grassland (草地学报), 2012, 20(1): 76-82 (in Chinese)
[11] Xu Y (许瑛), Chen F-D (陈发棣). The LT₅₀ and cold tolerance adaptability of chrysanthemum during a natural drop in temperature. Acta Horticulturae Sinica (园艺学报), 2008, 35(4): 559-564 (in Chinese)
[12] Deng R-J (邓仁菊), Fan J-X (范建新), Wang Y-Q (王永清), et al. Semilethal temperature of Pitaya under low temperature stress and evaluation on their cold resistance. Plant Physiology Journal (植物生理学报), 2014, 50(11): 1742-1748 (in Chinese)
[13] Gao J-F (高俊凤). Introduction of Plant Physiology Experiments. Beijing: Higher Education Press, 2006 (in Chinese)
[14] Zou Q (邹琦). The Experimental Guide for Plant Physiology. Beijing: China Agriculture Press, 2000 (in Chinese)
[15] Li H-S (李合生). Principles and Techniques of Plant Physiological Biochemical Experiment. Beijing: Higher Education Press, 2000: 258-260 (in Chinese)
[16] Zhang S-Q (张蜀秋), Li Y (李云), Wu W-H (武维华). Experimental Technology of Plant Physiology. Beijing: Science Press, 2011 (in Chinese)
[17] Wang R-X (王瑞雪). Effect of Low Temperature on the Physiological Characteristics of Acacia Hardy Clones and Comprehensive Evaluation. Master Thesis. Fuzhou: Fujian Agriculture and Forestry University, 2014 (in Chinese)
[18] Wang L (王玲) , Wang C-L (王春雷), Ma X-J (马喜娟), et al. Study on cold-resistance of new Weigela varieties. Journal of Northeast Forestry University (东北林业大学学报), 2012, 40(12): 43-46 (in Chinese)
[19] Wang G-Q (王冠群), Li D-Q (李丹青), Zhang J-P (张佳平), et al. Comparison of cold tolerance within 6 cultivars of Iris germanica. Acta Horticulturae Sinica (园艺学报), 2014, 41(4): 773-780 (in Chinese)
[20] Gao C-H (高灿红), Hu J (胡晋), Zheng Y-Y (郑昀晔), et al. Antioxidant enzyme activities and proline content in maize seedling and their relationships to cold endurance. Chinese Journal of Applied Ecology (应用生态学报), 2006, 17(6): 1045-1050 (in Chinese)
[21] Jiang M-Y (蒋明义), Guo S-C (郭绍川), Zhang X-M (张学明). Anti-oxidation effect of the accumulation of proline in rice seedlings exposed to oxidative stress. Acta Phytophysiologica Sinica (植物生理学报), 1997, 23(4): 347-352 (in Chinese)
[22] Yao H-M (姚海梅), Li Y-S (李永生), Zhang T-Z (张同祯), et al. Effects of combined drought and salinity stress on germination and physiological characte-ristics of maize (Zea mays). Chinese Journal of Applied Ecology (应用生态学报), 2016, 27(7): 2301-2307 (in Chinese)
[23] Xiang K (相昆), Zhang M-Y (张美勇), Xu Y (徐颖), et al. Cold tolerance of walnut cultivars: A comprehensive evaluation. Chinese Journal of Applied Ecology (应用生态学报), 2011, 22(9): 2325-2330 (in Chinese)
[24] Meng Q-W (孟庆伟), Gao H-Y (高辉远). Plant Physiology. Beijing: China Agriculture Press, 2011 (in Chinese)
[25] Li Y-B (李轶冰), Yang S-Q (杨顺强), Ren G-X (任广鑫), et al. Changes analysis in physiological properties of several gramineous grass species and cold-resis-tance comparison on under cold stress. Acta Ecologica Sinica (生态学报), 2009, 29(3): 1341-1347 (in Chinese)
[26] Wu N (吴娜), Zhou H-J (周怀军), Xiao F (肖芳). Changes of water content and soluble sugar in the leaves of three evergreen broad-leaf plants of Celastraceae during overwintering. Journal of Northwest Forestry University (西北林学院学报), 2006, 21(4): 36-38 (in Chinese)
[27] Zhou Y-H (周艳虹), Yu J-Q (喻景权), Qian Q-Q (钱琼秋), et al. Effects of chilling and low light on cucumber seedlings growth and their antioxidative enzyme activities. Chinese Journal of Applied Ecology (应用生态学报), 2003, 14(6): 921-924 (in Chinese)
[28] Jiang H (姜慧), Xu Y-C (徐迎春), Li Y-R (李永荣), et al. The study on cold resistance of different Citrus medica strains. Acta Horticulturae Sinica (园艺学报), 2012, 39(3): 525-532 (in Chinese)
[29] Yang J (杨静), Wang H-T (王华田), Song C-D (宋承东). Effect of durative low temperature on physiological and biochemical characteristics of Photinia glabra var. Rubens. Journal of Jiangxi Agricultural University (江西农业大学学报), 2007, 29(6): 992-998 (in Chinese)
[30] Li Y-F (李远发), Wang L-H (王凌晖), Tang C-H (唐春红), et al. Physiological responses on chilling stress of Jatropha curcas seedlings from different provenance. Journal of Northwest Forestry University (西北林学院学报), 2011, 26(5): 35-40 (in Chinese)
[31] Liu W (刘伟), Ai X-Z (艾希珍), Liang W-J (梁文娟), et al. Effects of salicylic acid on the leaf photosynthesis and autioxidant enzyme activities of cucumber seedling under low temperature and light intensity. Chinese Journal of Applied Ecology (应用生态学报), 2009, 20(2): 441-445 (in Chinese)