Effects of manure application on the diversity of corn root endophytic bacterial communities at seedling stage in eroded Mollisols.

doi:10.13287/j.1001-9332.201608.012

Chinese Journal of Applied Ecology ›› 2016, Vol. 27 ›› Issue (8): 2663-2669.doi: 10.13287/j.1001-9332.201608.012

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Effects of manure application on the diversity of corn root endophytic bacterial communities at seedling stage in eroded Mollisols.

YU Jiang^1,2, YU Zhen-hua¹, IKENAGA Makoto³, SAKAI Masao³, LIU Xiao-bing¹, WANG Guang-hua^1*

¹Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China;
²College of Agronomy, Northeast Agricultural University, Harbin 150030, China;
³Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan

Received:2015-12-25 Published:2016-08-18
Contact: * E-mail: wanggh@iga.ac.cn
Supported by:
This work was supported by National Science & Technology Pillar Program of China (2014BAD11B01-A01) and Grand Project of Heilongjiang Provincial Department of Science and Technology, China (GA14B101-A01).

Abstract

Abstract: In order to investigate the change of root endophytic bacterial communities under soil erosion condition, and to evaluate the response of root endophytic bacteria to manure fertilizer, we adopted the LNA-PCR clamping and 454 bar-coded pyrosequencing methods to study the corn root endophytic bacterial communities under 30 cm topsoil erosion and manure fertilization conditions. No topsoil removing (0 cm) and only chemical fertilizer treatment were used as control. A total of 37820 valid sequences of 16S rDNA were obtained, mainly distributed in 4 phyla, 35 classes, 214 genera and 782 OTUs. The dominant phyla were Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes, but their proportions varied in different samples. The diversity of corn root endophytic bacteria decreased in soil erosion condition. In the topsoil removing soil and no erosion soil, the diversity of corn root endophytic bacterial communities increased by manure application, and the effect was more obvious in the topsoil removing soil.

YU Jiang, YU Zhen-hua, IKENAGA Makoto, SAKAI Masao, LIU Xiao-bing, WANG Guang-hua. Effects of manure application on the diversity of corn root endophytic bacterial communities at seedling stage in eroded Mollisols.[J]. Chinese Journal of Applied Ecology, 2016, 27(8): 2663-2669.

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Effects of manure application on the diversity of corn root endophytic bacterial communities at seedling stage in eroded Mollisols.

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