Isolation, identification, and degradation characteristics of three effective PAHs degradation strains

doi:10.13287/j.1001-9332.202112.028

Abstract

Abstract: Isolating dominant strains for the degradation of polycyclic aromatic hydrocarbons (PAHs) is of great practical significance for the restoration of ecosystem polluted by PAHs. A total of 11 strains with capacity of degrading PAHs were obtained from soil polluted by PAHs around a coking plant, by enrichment culture, acclimation, and plate isolation. Three of them with effective PAH-degrading capability were identified and screened out by morphological observation, physiobiochemical characterization, and 16S rRNA gene sequencing, and respectively, named as DJ-3, DJ-8 and DJ-10. Based on the results of 16S rRNA gene sequencing, DJ-3, DJ-8, and DJ-10 were identified as Pseudomonas sp. Klebsiella sp., and Bacillus sp. The degradation rate of phenanthrene (200 mg·L^-1), pyrene (200 mg·L^-1), and naphthol (160 mg·L^-1) by three strains (DJ-3, DJ-8 and DJ-10) after seven-day incubation were 48.9%-65.9%, 38.9%-43.1%, and 57.6%-64.9%, respectively. The degradation rates of mixed PAHs sample (1200 mg·L^-1) by three strains were 49.1%, 44.5%, and 53.9%, which were significantly higher than other eight strains, indicating that they were highly effective in PAHs degradation. There was no antagonistic relationship among the three strains. This study would lay a foundation for building efficient PAHs degrading strains and improve the in situ bioremediation of PAHs contaminated soil.

Key words: coking plant, polycyclic aromatic hydrocarbons, degrading strains, isolation and identification, degradation characteristics

YAN Shuang-dui, LIU Li-jun, CAO Yan-zhuan, YAN Qiu-yan, DONG Xin-yu, HONG Jian-ping. Isolation, identification, and degradation characteristics of three effective PAHs degradation strains[J]. Chinese Journal of Applied Ecology, 2021, 32(12): 4439-4446.

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