Abstract: Eight populations of Plantago asiatica collected from Zhejiang were analyzed using random amplified polymorphic DNA (RAPD). Eighteen of the 60 random primers were selected.186 bands were amplified by the 18 primers,and 68.70% of them were polymorphic. Jaccard coefficient was applied to compare the genetic of these eight populations based on the RAPDdata. Three-dimensional ordination plot of the eight populations based on the Jaccard coefficient was constructed by using principal axes analysis (PAA). The results showed that the genetic differentiation of eight populations could be explained to a certain extent by their geographical location and altitude.