Effects of marine bacteria on the growth and toxin production of red-tide algae under different pH and salinities

Abstract

Abstract: The effects of strain S₁₀ isolated from sediments of Xiamen Western Sea Area on the growth and paralytic shellfish poison (PSP) production of Alexandrium tamarense at different pHand salinities were studied. The results showed that the alga grew well at pH6～8 and at salinity of 20～34. The toxicity of A. tamarense varied markedly at different pH and salinities:it decreased with increasing pH,while increased with salinity and reached its peak value at the salinity of 30, and then declined. The strain S₁₀ inhibited the growth and the PSP production of A. tamarense at different pH and salinities.It had the best inhibitory function on the growth of A. tamarense at pH7 and salinity of 34. The best inhibitory function on the PSP production of A.tamarense was at pH7, but this inhibitory function was not related to salinity.

Key words: Marine bacteria, Alexandrium tamarense, Paralytic shellfish poison

CLC Number:

Q143

SU Jianqiang, ZHENG Tianling, HU Zhong, XU Jingsen, YU Zhiming, SONG Xiuxian. Effects of marine bacteria on the growth and toxin production of red-tide algae under different pH and salinities[J]. Chinese Journal of Applied Ecology, 2003, (7): 1161-1164.

References

[1] Bates SS, Douglas DJ, Doucette GJ, Leger C. 1995. Enhancement of domoic acid production by reintroducing bacteria to axenic cultures of the diatom Pseudonitzschia multiseries. Nat Toxins,3:428～435
[2] Cembella AD, Milenkovic L, Douctte G,et al. 1995. In vitro biochemical methods and mammalian bioassays for Phycotoxins. In:Hallegraeff GM, Anderson DM, Cembella AD, eds. Manual on Harmful Marine Microalgae, Intergovernmental Oceanographic Commission Manuals and Guides No. 33. UNESCO:177～223
[3] Doucette GJ. 1995. Assessment of the interaction of prokaryotic cells with harmful algal species. In:Lassus P, Arzul G, Erard E, Gentien P, Marcaillou C eds. Harmful Marine Algal Blooms. Paris:Lavoisier Science Publ. 385～394
[4] Docucette GJ, Kodama M, Gallacher S. 1998. Bacterial interaction with harmful algal bloom species:Bloom ecology, toxigenesis and cytology. In:Anderson DM, Cembella AD, Hallegraeff GM eds. Physiological Ecology of Harmful Algal Bloom. NATO ASI Series, Vol. G41.Berlin,Heidelberg:Springer-Verlag.619～647
[5] Flynn KJ, Flynn K, John EH,et al.1996. Changes in toxins, intracellular and dissolved free amino acids of the toxic dinoflagellate Gymnodinium catenatum in response to changes in inorganic nutrients and salinity.J Plank Res,18:2093～2111
[6] Hold GL, Smith EA, Birkbeck TH, Gallacher S. 2001. Comparison of paralytic shellfish toxin(PST) production by the dinoflagellates Alexandrium lusitanicum NEPCC 253 and Alexandrium tamarense NEPCC 407 in the presence and absence of bacteria.FEMS Microbiol Ecol,36:223～234
[7] Hold GL, Smith EA, Rappe MS,et al. 2001. Characterization of bacterial communities associated with toxic and non-toxic dinoflagellates:Alexandrium spp. and Scrippsiella trochoidea. FEMS Microbiol Ecol,37:161～173
[8] Indrasena WM, Gill TA. 2000. Storage stability of paralytic shellfish poisoning toxins.Food Chem,71:71～77
[9] Jiang T-J(江天久), Huang W-J(黄伟建), Wang C-H(王朝晖),et al. 2000. Effects of water temperature, salinity and pH on growth and toxicity of Alexandrium tamarense(Lebour) balech(Dapeng strain).Chin J Appl Environ Biol(应用与环境生物学报),6(2):151～154(in Chinese)
[10] Li R-X(李瑞香), Xia B(夏滨).1996. Two toxic dinoflagellates in Jiaozhou Bay-Alexandrium tamarense and Alexandrium catenella. In:Zhu M-Y(朱明远), Li R-X(李瑞香), Wang F(王飞), eds. Red-tide Research of China. Qingdao:Qingdao Press. 36～41(in Chinese)
[11] Lian Y-W(连玉武), Wang Y-L(王艳丽), Zheng T-L(郑天凌),et al. 1999. Advance in the research on interaction between red-tide algae and bacteria.Mar Sci(海洋科学), 1:35～39(in Chinese)
[12] Lin W(林伟), Zhou M-J(周名江). 2001. Effect of marine bacteria on harmful algal bloom.Mar Sci(海洋科学),25(3):34～38(in Chinese)
[13] Lin Y-S(林元烧), Cao W-Q(曹文清), Sameer T,et al. 2002. Studies on dinoflagellate cysts and their distribution in Xiamen Western Harbour.Oceanol Limnol Sin(海洋与湖沼),33(4):407～414(in Chinese)
[14] Luo Y-P(罗岳平), Li Y-J(李益健), Tang Z-Q(谭智群). 1996. Adhesion behaviour of bacterial and algae and its ecological significance.Chin J Ecol(生态学杂志),15(5):55～61(in Chinese)
[15] Mayali X, Docucette GJ. 2002. Microbial community interactions and population dynamoics of an algicidal bacterium active against Karenia brevis. Harmful Algae,1:277～293
[16] Riemann L, Steward GF, Azam F. 2000. Dynamics of bacterial community composition and activity during a Mesocom Diatom bloom.Appl Environ Microbiol,66(2):578～587
[17] Ravn H, Anthoni U, Christophersen C,et al. 1995. Standardized extraction method for paralytic shellfish toxins in phytoplankton.J Appl Phycol,7:589～594
[18] Usup G, Kulis DM, Anderson DM. 1994. Growth and toxin production of the toxic dinoflagellate Pyrodinium bahamense var.compressum in laboratory cultures.Nat Tox,2:254～262
[19] White AW.1978. Salinity effects on growth and toxin content of Gonyaulax excavata. A marine dinoflagellate causing paralytic shellfish poisoning.J Phycol,14:475～479
[20] Wu G(吴刚), Xi Y(席宇), Zhao Y-J(赵以军). 2002. The latest development of research on algae-lysing bacteria.Res Environ Sci(环境科学研究), 15(5):43～46(in Chinese)
[21] Zhao Y-J(赵以军), Liu Y-D(刘永定). 1996. Possible microbial control on the adverse impacts of algae-Current information about the relationship between algae and microbes.Acta Hydrobiol Sin(水生生物学报), 20(2):174～181(in Chinese)