Isolation and characteristics of PAHs-degrading strains

Abstract

Abstract: The predominant indigenous microbial population and the strain ZL5 PAHS-degrading were obtained by selective enrichment culture from Liaohe viscous oil-contaminated field.The sequence analysis of 16s rDNA showed that strain ZL5 belonged to Sphingomonas sp.The microbial population and the strain could degrade phenanthrene well.95.28% and 69.24% of phenanthrene in the medium were degraded in 120hrs.However, the degradation of pyrene by the microbial population or by the strain was low.The degradation rate of phenanthrene or pyrene by the microbial population or the strain was greatly increased while glucose was used as an added substrate.The effect was closely related to the amount of glucose.If the concentration were too high, glucose would show inhibition effect on the degradation of PAHs.Therefore, it was necessary to control the amount of glucose while using glucose as an added substrate to increase degradation of phenanthrene or pyrene.

Key words: Polycyclic aromatic hydrocarbons (PAHs), Glucose, Sphingonumas sp.

CLC Number:

X172

ZHANG Jie, LIU Yongsheng, MENG Ling, SHAO Lidong, ZHANG Zhongze . Isolation and characteristics of PAHs-degrading strains[J]. Chinese Journal of Applied Ecology, 2003, (10): 1783-1786.

References

[1] Boldrin B,Thiem A,Fritsche C.1993.Degradation of phenanthrene,fluorene,fluoranthene,and pyrene by a Mycobacterium sp.Appl Environ Microbiol,59:1927～1930
[2] Cho JC,Kim SJ.2001.Detection of mega plasmid from polycyclic aromatic hydrocarbon-degrading Sphingomonas sp.strain ks14.J Mol Microbiol Biotechnol,3(4):503～506
[3] Daun G,Lenke F,Reuss M,et al.1998.Biological treatment of TNT-contaminated soilⅠ.Anaerobic co-metabolic reduction and interaction of TNT and metabolites with soil components.Environ Sci Technol,32:1956～1963
[4] Deziel E,Paquette G,Villemur R,et al.1996.Biosurfactant production by a soil Pseudomonas strain growing on polycyclic aromatic hydrocarbons.Appl Environ Microbiol,62:1908～1912.
[5] Fredrickson JK,Balkwill DL,Drake GR.1995.Aromatic-degrading Sphingomonas isolates from the deep subsurface.Appl Environ Microbiol,61(5):1917～1922
[6] Gong Z-Q(巩宗强),Li P-J(李培军),Wang X(王新),et al.2001.Co-metabolic degradation of pyrene in soil.Chin J Appl Ecol(应用生态学报),12(3):447～450(in Chinese)
[7] Gottschalk G.1979.Bacterial Metabolism.New York:Springer-Verlag,Inc.
[8] Goyal AK,Zylstra GJ.1996.Molecular cloning of novel genes for polycyclic aromatic hydrocarbon degradation from Comamonas testosteroni GZ39.Appl Environ Microbiol,62(1):230～236
[9] James EB,Douglas GC.1988.Effects of coocurring aromatic hydrocarbon on degradation of individual polycyclic aromatic hydrocarbons in marine sediments slurries.Appl Environ Microbiol,54(7):1649～1655
[10] Jiang C-L(姜昌亮),Sun T-H(孙铁珩),Li P-J(李培军),et al.2001.An off site petroleum-contaminated soil bioremediation technology: Soil composting in windrow.Chin J Appl Ecol(应用生态学报),12(2): 279～282 (in Chinese)
[11] Pettigrew CA,Breen A,Corcoran C,et al.1990.Chlorinated biphenyl mineralization by individual populations and consortia of freshwater Bacteria.Appl Environ Microbiol,56(7):2036～2045
[12] Tongpim S,Pickard MA.1999.Cometabolic oxidation of phenanthrene to phenanthrene trans-9,10-dihydrodiol by mycobacterium strain S1 growing on anthracene in the presence of phenanthrene.Can J Microbiol,45(5):369～376
[13] Wang SJ,Loh KC.1999.Facilitation of cometabolic degradation of 4-chlorophenol using glucose as an added growth substrate.Biodegradation,10(4):261～269
[14] Wei C-H(韦朝海),Hou Y(侯轶),Ren Y(任缘),et al.2000.Bio-cooperation effect and mixing substrates in the aerobic degradation of nitrobenzene.Chin Environ Sci(中国环境科学),20(3):241～244(in Chinese)
[15] Weisburg WG,Barns SM,Pelletier DA.1991.16s ribosomal DNA amplification for phylogenetic study.J Bacteriol,173(2):697～703