Semi-nested LP-PCR-SSCP identification of arbuscular endomycorrhizal fungi diversity of Alnus at Changbai Mountain

Abstract

Abstract: The diversity of arbuscular endomycorrhizal fungi colonized on three species of Alnus at Changbai Mountain was identified based on coupling the sensitivity of semi-nested LP-PCR and the specificity afforded by Single Strand Conformation Polymorphism analysis. It is suggested that altitudes and species of Alnus were not the important factors deciding the distribution of AMF in the grade of family, and there was at least one species of Glomaceae, Glomus intraradix, dominated in Alnus at Changbai Mountain.

Key words: Alnus, Arbuscular endomycorrhizal fungi, Semi-nested LP-PCR-SSCP

CLC Number:

Q949.32

LI Yiye, HE Xingyuan, ZHANG Zhongze, ZHANG Chenggang . Semi-nested LP-PCR-SSCP identification of arbuscular endomycorrhizal fungi diversity of Alnus at Changbai Mountain[J]. Chinese Journal of Applied Ecology, 2003, (11): 1837-1841.

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