Abstract: By using two-dimensional electrophoresis and mass spectrometry, this paper studied the changes of the proteome in nurse seed grafted unions of Camellia oleifera at its different developmental stages (4, 8, 16, 22, 29, and 35 days after grafting). A total of 40 differential proteins were detected, 9 of which could be related to the healing of nurse seed grafted unions. Grafting stimulated the grafted union’s gene transcription and protein translation, signal transduction, energy metabolism, and compound synthesis and hormone regulation, and activated some enzymes activities, enhanced cell resistance, and promoted the union’s healing.

Key words: Camellia oleifera, nurse seed grafting, proteomics, healing.