Abstract: The application of Brachydanio rerio embryo development technique to research on environmental science was summarized in this paper.This testing technique was approved as one of standard methods for chemical toxicity test,especially for teratogensis,by international organizations (OECD,DIN).There are many advantages of this testing, e.g.,low cost, easy operation,high sensitivity and multiple sensible endpoints,comparing with other toxicity testing.Also,it was used to judge toxic mechanism of chemicals.The embryo develompent of zebrafish can be affected obviously by heavy metal,pesticide,organic reagent and complex chemicals.Among them,Cu and Hg appeared stronger toxicity,and Cr was weaker for heavy metals.TPTAand Lindane were higher toxicity within pesticide,and they were higher toxicity if the organic reagent with one or more halogen substituent (s) and aminobenzene.These results were correspondent with other toxicity testing,and it showed higher sensitivity,especially a sublethal endpoint was selected.Therefore,it can be expected to test toxicity and teratogensis on mixed pollutant monitoring.

Key words: Brachydanio rerio, Embryo development, Toxicity evaluation