Welcome to Chinese Journal of Applied Ecology! Today is Share:

Chinese Journal of Applied Ecology ›› 2000, Vol. ›› Issue (5): 791-794.

Previous Articles     Next Articles

Factors affecting transormation of Agrobacterium tumefaciens and their application on cereals

KONG Yingzhen, ZHOU Gongke, WANG Genxuan, WANG Yafu   

  1. State Key Laboratory of Arid Agroecology, Lanzhou University, Lanzhou 730000
  • Received:1999-10-29 Revised:1999-12-21

Abstract: Agrobacterium tumefaciens mediated genetic transformation is the method most widely used in plant transformation.How to improve its transformation efficiency and extend its host range to include most cereals is what people concern about.There are many factors that influence the transformation effciency,including the wounding response of plants,attachment of bacteria,induction of virulent gene,DNA repair and replication activity of plant cells,state of explant,etc. Recent research has proved that cerelas can be transformed effectively by A.tumefaciens under suitable conditions.This paper reviewed the recent progress in the two aspects.

Key words: Agrobacterium tumefaciens, Transformation efficiency, Cereals