Accumulation of phenolic acids in continuously cropped cucumber soil and their effects on soil microbes and enzyme activities

Abstract

Abstract: The study showed that in solar greenhouse continuously cropped cucumber soil,phenolic acids p-hydroxybenzoic acid,ferulic acid and benzoic acid had an obvious accumulation with increasing cropping year,and their contents were significantly higher after continuously cropped for 5～9 years than for 1～3 years.With the increasing concentration of treated exogenous phenolic acids,the amounts of bacteria,actinomycetes,total microbes,ammonibacteria,and nitrifying bacteria in cucumber root area increased first,but decreased then.Soil bacteria and actinomycetes had the largest amount at the concentration of 80 μg phenolic acids.g^-1 soil,while soil fungi(including Fusarium and Phytophthora) increased rapidly when the concentration of phenolic acids was lower than 120 μg·g^-1 soil.With increasing phenolic acids concentration,soil enzyme activities also increased first but decreased then,with the peak values differed in different treatments.

Key words: Solar greenhouse, Continuously cropped soil, Phenolic acids, Microflora, Enzyme activity

CLC Number:

S642.2

MA Yunhua, WANG Xiufeng, WEI Min, QI Yanfeng, LI Tianlai . Accumulation of phenolic acids in continuously cropped cucumber soil and their effects on soil microbes and enzyme activities[J]. Chinese Journal of Applied Ecology, 2005, 16(11): 2149-2153.

References

[1] Blum U,Shafer SR.1988.Microbial population and phenolic acids in soils.Soil Biol Biochem,20(6):793～800
[2] Du Y-J(杜英君),Jin Y-H(靳月华).1999.Simulations of allelopathy in continuous cropping of soybean.Chin J Appl Ecol(应用生态学报),10(2):209～212(in Chinese)
[3] Fu H-L(付慧兰),Zou Y-J(邹永久),Yang Z-M(杨振明).1997.Soil pH of continuous cropping soybean and soil enzyme activity.Soybean Sci(大豆科学),16(2):156～161(in Chinese)
[4] Gao Z-Q(高子勤),Zhang S-X(张淑香).1998.Continous cropping obstacle and rhizospheric microecology I.Root exudares and their ecological effects.Chin J Appl Ecol(应用生态学报),9(5):549～554(in Chinese)
[5] Guan S-Y(关松荫).1986.Soil Enzymes and the Research Methods.Beijing:Agricultural Press.(in Chinese)
[6] Ju H-Y(鞠会艳),Hah L-M(韩丽梅),Wang S-Q(王树起).2002.Allelopathic effect of root exudates on pathogenic fungi of root rot in continuous cropping soybean.Chin J Appl Ecol(应用生态学报),13(6):723～727(in Chinese)
[7] Lü W-G(吕卫光),Zhang C-L(张春兰),Yuan F(袁飞).2002.Mechanism of allelochemicals inhibiting continuous cropping cucumber growth.Sci Agric Sina(中国农业科学),35(1):106～109(in Chinese)
[8] Ma R-X(马瑞霞).1999.Study on influence of allelochemicals on activity of nitrate reductases.Environ Sci(环境科学),20(1):80～83
[9] Ma R-X(马瑞霞),Liu X-F(刘秀芬),Yuan G-L(袁光林).1996.Study on allelochemicals in the process of decomposition of wheat straw by microorganisms andtheir bioactivity.Acta Ecol Sin(生态学报),16(6):632～639(in Chinese)
[10] Ma R-X(马瑞霞),Liu X-F(刘秀芬),Yuan G-L(袁光林).1997.Study onthe allelochemicals produced by bacteria and fungi in rhizosphere andtheir bioactivity.Acta Ecol Sin(生态学报),17(4):449 ～ 451 (in Chinese)
[11] Murary AH. 1996. Effect of simple phenolic compounds of heather (Calluna vulgaris)on rumen microbial activity in vitro. J Chem Ecol, (22):1493～ 1505
[12] Szajdak L. 1994. Phenolic acids in brown soils under continuous cropping of rye and crop rotation. Polish J Soil Sci, 27(2):113～121
[13] Wang Q(王倩),Li X-L(李晓林).2003.Effects of benzoic and cinnamon acids on watermelon seedling growth and fusarium wilt occurrence.J Chin Agric Univ(中国农业大学学报),8(1):83～86(in Chinese)
[14] Wu E(吴萼),LiuX-Y(刘晓艳),ZhuX-R(祝心如).1999.The effects of groupsin phenolic compounds oninhibition of nitrification in soil.Environ Chem(环境化学),18(5):398～403(in Chinese)
[15] Wu F-Z(吴凤芝),Huang C-H(黄彩红),Zhao F-Y(赵凤艳).2002.Effects of phenolic acids on growth and activities of membrahe protective enzymes of cucumber seedlings.Sci Agric Sin(中国农业科学),35(7):821～825(in Chinese)
[16] Wu F-Z(吴凤芝),Zhao F-Y(赵凤艳).2003.Study on root exudates and continues cropping obstacle.J Northeast Agric Univ(东北农业大学学报),34(1):114～118(in Chinese)
[17] Wu F-Z(吴凤芝),Zhao F-Y(赵凤艳),Liu Y-Y(刘元英).2000.On the reasons of continuous cropping obstacles in vegetable facility gardening.J Northeast Agric Univ(东北农业大学学报),3l(3):241～247(in Chinese)
[18] Yu JQ.1997.Effects of root exudates of cucumber and allelochemicals onion uptake by cucumber seedlings.J Chem Ecol,23(3):817～827
[19] Yu J-Q(喻景权),Du Y-S(杜尧舜).2000.Soil sickness problem in the sustainable development for the protected production of vegetables.J Shenyang Agric Univ(沈阳农业大学学报),31(1):124～126(in Chinese)
[20] Yu JQ,Matsui Y.1994.Phytotoxic substances in root exudates of cucumber.J Chem E.col,20(1):21～31
[21] Zhang S-X(张淑香),Gao Z-Q(高子勤),Liu H-L(刘海玲).2000.Continuous cropping obstacle and rhizospheric microecology Ⅲ.Soil phenolic acids and their biological effect.Chin J Appl Ecol (应用生态学报),11(5):741～744(in Chinese)
[22] Zhou Z-H(周志红),Luo S-M(骆世明),Mu Z-P(牟子平).1997.Allelopathic effect of tomato.Chin J Appl Ecol(应用生态学报),8(4):445～449(in Chinese)
[23] Zhu L-X(朱丽霞),Zhang J-E(章家恩),Liu W-G(刘文高).2003.Review of studies on interactions between root exudates and rhizopheric microorganisms.Ecol Environ(生态环境),12(1):102～105(in Chinese)