Population fluctuation of main pathogens and their antagonistic bacteria in cucumber rhizosphere

Abstract

Abstract: The investigation on the population fluctuation of main pathogens Pythium spp.and Fusarium oxysporum f.sp.cucumerinum in the rhizosphere of four cucumber varieties showed that there was a significant difference in the population number of pathogens for different cucumber varieties and for different growth stages of the same variety,which was in accordance with the regular disease incidence pattern.The population number of Pythium spp.was significantly higher at cucumber creeping stage than at its other growth stages,while that of Fusarium oxysporum f.sp.cucumerinum was the highest at cucumber principal-fruiting stage.There was a closer positive relationship between two test pathogens and their antagonistic bacteria,with the correlation coefficient being 0.95 and 0.81,respectively.

Key words: Cucumber, Rhizosphere, Pathogen, Antagonistic bacteria, Population number

CLC Number:

LIANG Jiangen, ZHANG Bingxin, YU Jingquan, SHI Jiang, CHEN Zhenyu . Population fluctuation of main pathogens and their antagonistic bacteria in cucumber rhizosphere[J]. Chinese Journal of Applied Ecology, 2005, 16(5): 911-914.

References

[1] Burke D, Hamerlynck E, Hahn D. 2002. Interactions among plant species and microorganisms in salt marsh sediments. Appl Environ Microbiol, 68(3):1157～1164
[2] Burke D, Hamerlynck E, Hahn D. 2003. Interactions between the salt marsh grass Spartina patens, arbuscular mycorrhizal fungi and sediment bacteria during the growing season. Soil Biol Biochem, 35(4):501～511
[3] Chen S-H(陈素华), Sun T-H(孙铁珩), Zhou X-Q(周启星).2002. Interaction between microorganism and heavy metals and its application. Chin J Appl Ecol (应用生态学报), 13(2) :239 ～242(in Chinese)
[4] Chen Z-Y (陈中义), Zhang J (张杰), Huang D-F (黄大肪).2003. Research progress on antagonistic mechanism and improvement of genetic engineering of genus Bacillus for plant disease biocontrol. Acta Pytopathol Sin (植物病理学报), 33(2):97 ～103(in Chinese)
[5] Feng J(冯洁),Chen Q-Y(陈其瑛),Shi L-Y(石磊岩), et al.1991. Studies on relationship between root exudates of cotton seedling and Fusarium wilt disease. Acta Gossypii Sin (棉花学报), 3 (1):89 ～96 (in Chinese)
[6] Folman LB, Postma J, van Veen JA. 2002. Ecophysiological characterization of rhizosphere bacterial communities at different root locations and plant developmental stages of cucumber grown on rockwool. Microbial Ecol, 42:586～597
[7] Hu F(胡锋), Li H-X(李辉信),Shi y-Y(史玉英), et al. 1998.Dynamics of soil biota in the rhizosphere of two wheat genotypes and their rhizosphere effect. Chin J Soil Sci (土壤通报), 29(3):133～135(in Chinese)
[8] Jia Z-H(贾志红), Sun M(孙敏), Yang Z-P(杨珍平), et al.2004. Influence of different fertilizers to crop rhizosphere microorganisms. Acta Agron Sin (作物学报), 30 (5):491 ～495 (in Chinese)
[9] Jiao R-Z(焦如珍), Yang C-D(杨承栋). 1999. The changes of the soil microorganism in rhizosphere and outside in different developing stages of the Chinese fir plantation. Sci Silvae Sin (林业科学),35(1) :53～59(in Chinese)
[10] Kari ED, James JG. 2001. Diversity of bacterial communities in the rhizosphere and root interior of field-grown genetically modified Brassica napus. FEMS Microbiol Ecol, 38(1):1～9
[11] Komada H. 1975. Development of a selective medium for quantitative isolation of Fusarium oxysporum from natural soil. Rev Plant Protect Res, 8:114～125
[12] Li H-L(李洪连), Wang S-Z(王守正),Zhang M-Z(张明智).1990. Cotton varieties resistant and susceptible to wilting and rhizosphere microorganism quantity. Henan Agric Univ(河南农业大学学报), 24(1) :49～56(in Chinese)
[13] Li Z-G(李高), Li L-M(李良谟), Pan y-H(潘映华), et al.1995. Influence of the root exudates of wheat shoots on the denitrifiers in rhisosphere. Acta Pedol Sin (土壤学报), 32(4) :408～413(in Chinese)
[14] Li Z-G(李鹏高), Pan Y-H(潘映华), Li L-M(李良谟). 1993. Dynamics bacteria and their enzyme activity in rhizosphere of wheat of different genotypes. Acta Pedol Sin (土壤学报), 30 (1):1 ～7 (in Chinese)
[15] Liao M(廖敏), Huang C-Y(黄昌勇). 2002. Microbial biomass affected by cadmium with the occurrence of organic acids in red soil. Chin J Appl Ecol (应用生态学报), 13(3) :300～302(in Chinese)
[16] Liu H-S(刘洪生), Song Q-H(宋秋华), Li F-M(李凤民). 2002.The roles of root exudation on rhizosphere nutrient and rhizosphere microorganisms. Acta Bot Boreal-Occident Sin (西北植物学报),22 (3):693 ～702 (in Chinese)
[17] Long J(龙健),Huang C-Y(黄昌勇),Teng Y(滕应),et al.2003. Microbial eco-characteristics of reclaimed mining wasteland in red soil area of southern China I. Effects on soil microbial activity.Chin J Appl Ecol (应用生态学报), 14 (11):1925 ～1928 (in Chinese)
[18] Lu S-Y (鲁素芸). 1993. Biological Control of Plant Disease. Beijing:Beijing Agricultural University Press. (in Chinese)
[19] Mahaffee WF, Kloepper JW. 1997. Temporal changes in the bacterial communities of soil, rhizosphere, and endorhiza associated with field-grown cucumber(Cucumber sativus L.). Microb Ecol, 34:210～223
[20] Shen H(沈宏),Cao Z-H(曹志洪),Xu B-S(徐本生).1999.Dynamics of soil microbial biomass and soil enzyme activity and their relationships during maize growth. Chin J Appl Ecol (应用生态学报), 10(4) :471～474(in Chinese)
[21] Song R(宋日), Wu C-S(吴春生), Mou J-M(牟金明), et al.2002. Efecct of maize stubble remaining in field on dynamics of soil microbial biomass C and soil enzyme activities. Chin J Appl Ecol(应用生态学报), 13(3) :303～306(in Chinese)
[22] Tang Q-Y(唐启义), Feng M-G (冯明光). 1997. Practical Statistics and DPS Date Processing System. Beijing:China Agricultural Press. (in Chinese)
[23] Tian Y-H(田永辉), Wei J(魏杰). 2001. Dynamic state of bacteria and enzyme activity in rhizosphere of tea different varieties. J Southwest Agric Sci (西南农业学报), 14(4) :63～66(in Chinese)
[24] van der Plaats-Niterink AJ. 1981. Monograph of the Genus pythium. Stud. Mycol. Natherlands:Centraalbwreau Voor Schimmelcu ltures Baarn.
[25] Wang C-X(王春霞), Wang D-B(王道本), Zhou Q(周启).1996. Isolation and screening of the plant growth-promoting in rhizobacteria of cotton. J Huazhong Agric Univ (华中农业大学学报), 15(3) :234(in Chinese)
[26] Wang Z-H(王忠华), Ye Q-F(叶庆富), Shu Q-Y(舒庆尧), et al.2002. Impact of root exudates from transgenic plants on soil microecosystem. Chin J Appl Ecol (应用生态学报), 13 (3):373 ～375(in Chine se)
[27] Wang Z-Y(王政逸), Zhang B-X(张炳欣), Lou B-G(楼兵干).1996. On the ecology of Pythium spp. in wheat rhizosphere in Zhejiang province. Acta Pytopathol Sin (植物病理学报), 26 (1):29～30(in Chinese)
[28] Xiong M-B(熊明彪), He J-P(何建平), Song G-Y(宋光煜).2002. Effect of root exudations on ecological distributin of rizospheric microorganisms. Chin J Soil Sci (土壤通报), 33(2):145～148(in Chinese)
[29] Yang C-H, David EC. 2000. Rhizosphere microbial communities structure in relation to root location and plant iron nutritional status. Appl Environ Microbiol, 66(1):345～351
[30] Yu S(俞慎),He Z-L(何振立),Huang C-Y(黄昌勇).2003.Advance in the research of soil microorganisms and their mediated processes under heavy metal stress. Chin J Appl Ecol (应用生态学报), 14(4):618 ～622(in Chinese)