Effects of exogenous Ca on some physiological characteristics of tomato (Lycopersicon esculentum) seedlings with different Ca sensitivity

Abstract

Abstract: Two tomato (Lycopersicon esculentum) cultivars with different Ca sensitivity were used to study the effects of exogenous Ca on their root activity,CaM content,chlorophyll a/b ratio,and reactive oxygen-scavenging enzymes' activities under different Ca concentrations in nutrient solution.The results showed that the root activity and CaM content of two cultivars increased with increasing Ca concentrations in the nutrient solution.The root activity and chlorophyll a/b ratio of Ca-insensitive cultivar Jiangshuyihao were higher than those of Ca-sensitive L-402 under extreme low Ca stress (1 and 4 mg稬^-1),while lower than those of L-402 when sufficient Ca was applied (100 mg稬^-1),indicating that Jiangshuyihao had a greater capability to bear the nutrient and light stress under low Ca stress.The SOD activity of Ca-insensitive cultivar Jiangshuyihao under 1 and 4 mg稬^-1 Ca was higher than that of L-402,while the activities of POD and CAT of L-402 were consistently higher than that of Jiangshuyihao under test Ca concentrations in the nutrient solution.Comparing with 100 mg稬^-1 Ca in solution,4 mg稬^-1 Ca led to an increase of CAT and POD activities and a decrease of SOD activity in the two cultivars,but the variation of SOD activity was greater in L-402 than in Jiangshuyihao,indicating that the system of defence enzymes in Ca-insensitive cultivar played a key role when suffered from Ca stress.

Key words: Ca sensitivity, Tomato seedlings, Root activity, Chlorophyll a/b, Reactive oxygen-scavenging enzymes

CLC Number:

Q945.3

DONG Caixia, ZHOU Jianmin, ZHAO Shijie, WANG Huoyan . Effects of exogenous Ca on some physiological characteristics of tomato (Lycopersicon esculentum) seedlings with different Ca sensitivity[J]. Chinese Journal of Applied Ecology, 2005, 16(2): 267-272.

References

[1] Bowler C, Van Montagu M, Inze D. 1992. Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol, 43:83～116
[2] Dong C-X(董彩霞), Zhou J-M(周键民), Fan X-H(范晓睴), et al. 2003. Effects of decrease of Ca application on Ca forms and content in the fruit of different calcium efficiency tomato cultivars in florescence. Plant Physiol Commun (植物生理学通讯), 39(2):128～130(in Chinese)
[3] Dong C-X(董彩霞), Zhou J-M(周键民), Wang H-Y(王火焰).2003. Difference of the sensitivity to Ca deficiency in nutrient solution among tomato cultivars. Acta Bot Borea-Occid Sin (西北植物学报), 23(5):777～782(in Chinese)
[4] Dong C-X(董彩霞),Zhou J-M(周健民), Wang Q-Y(王庆亚), et al. 2003. Effects of Ca deficiency on the Ca absorption and distribution in plant and the anatomical structure of stem in tomato seedlings with different Ca efficiency. Plant Nutr Fert Sci (植物营养与肥料学报), 9(2):233～237(in Chinese)
[5] Jian LC, Li JH, Li PH. 1997. Is Ca²⁺ homeostasis essential in the cold acclimation of winter wheat seedlings? In:Bold Z, ed. Cereal Adaptation to Low Temperature Stress in Controlled Environments. Martonvasar, Hungary:Agr Res Inst. Hungarian Acad Sci.69～71
[6] Jiang C-D(姜闯道),Gao H-Y(高辉远),Zou Q(邹崎).2002.Increase in excitation energy dissipation by iron deficiency in soybean leaves. J Plant Physiol Mol Biol (植物生理与分子生物学学报), 28(2):127～132. (in Chinese)
[7] Jiang M-Y(蒋明义),Jing J-H(荆家海), Wang S-T(王韶唐).1991. Effects of osmotic stress on membrane-lipid peroxidation and endogenous protective systems in rice seedlings. Acta Phytophysiol Sin (植物生理学报), 17(1):80～84(in Chinese)
[8] Li H-S(李合生). 2000. Principles and Techniques of Plant Physiological Biochemical Experiment. Beijing. Higher Education Press.164～167(in Chinese)
[9] Liu H-Y(刘慧英), Zhu Z-J(朱祝军), Lü G-H(吕国华). 2004. Effect of low temperature stress on chilling tolerance and protective system against active oxygen of grafted watermelon. Chin J Appl Ecol (应用生态学报), 15(4):659～662(in Chinese)
[10] Niyougi KK. 1999. Photoprotection revisited:Genetic and molecular approaches. Annu Rev Plant Physiol Plant Mol Biol, 50:333～359
[11] Sandmann G, Richard M. 1983. Iron-sulfur centers and activities of the photosynthetic electron transport chain in iron-deficient cultures of the blue-green alga aphanocapsa. Plant Physiol, 73:724～728
[12] Shen Q-R(沈其荣), Zhu Y-Y(朱毅勇), Xie X-D(谢学东), et al.2000. The role of Ca²⁺ in the systemic resistance of cucumber young leaf induced by phosphate nutrition. Plant Nutr Fert Sci (植物营养与肥料学报), 6(3):280～286(in Chinese)
[13] Shi M-T(施木田),Chen D-K(陈东凯).2004. Effects of zinc and boron nutrition on balsam pear(Momordica charantia) yield and quality, and polyamines, hormone, and senescence of its leaves. Chin J Appl Ecol(应用生态学报), 15(1):77～80(in Chinese)
[14] Tang Z-C(汤章城). 1999.Current Plant Physiological Experiment Manual. Beijing:Science Press. 251～252 (in Chinese)
[15] Wang A-G (王爱国). 1998. The oxygen metabolism in plant. In:Yu S-W(余叔文), Tang Z-C(汤章城), eds. Plant Physiology and Molecular Biology. Beijing:Science Press. 366～389 (in Chinese)
[16] Wang H(王红), Jian L-C(简令成), Zhang J-R(张举仁). 1994.Changes of the level of Ca²⁺ in cells of rice seedlings under low temperature stress. Acta Bot Sin (植物学报), 36(8):587～591(in Chinese)
[17] Zhang F-Q(张芬琴), Shen Z-G(沈振国), Liu Y-L(刘友良).2000. Effects of Al³⁺ and Al³⁺ + Ca²⁺ on ATPases and membrane fluidities of plasma membrane and tonoplast vesicles from root tips of wheat seedlings. Acta Phytophysiol Sin (植物生理学报), 26(2):105～110(in Chinese)
[18] Zhang Y-T(张运涛). 1996. The role of calcium in salt toxicity of plants. J Hebei Agrotech n Teachers Coll(河北农业技术师范学院学报), 10(3):61～66(in Chinese)
[19] Zheng Q-S(郑青松), Wang R-L(王仁雷), Liu Y-L(刘友良).2001. Effects of Ca²⁺ on absorption and distribution of ions in salt treated cotton seedlings. Acta Phytophysiol Sin (植物生理学报),27(4):325～330(in Chinese)
[20] Zou Q(邹琦). 1995. Plant Physiology and Biological Chemistry Manual. Beijing:China Agricultural Press. 32～33 (in Chinese)