Production and properties of chitinase from Beauveria bassiana Bb174 in solid state fermentation

Abstract

Abstract: This paper studied the chitinase production of Beauveria bassiana Bb174 under solid state fermentation condition.The optimal medium consisted of wheat bran and silkworm chrysalis at the ratio of 4:1,supplemented with 1 g peptone·L^-1 as nitrogen source and some other mineral nutrients.The enzyme activity reached 126 units per gram dry medium after cultured for 2 days at 28 ℃ and natural pH by inoculated 3 ml spore suspension into this medium.The optimal temperature and pH for chintinase production were 40 ℃ and 5.0,respectively.The temperature to lose 50% activity of the enzyme was 48 ℃ after incubated at 30～70 ℃ for 1 h.The enzyme was stable at 30～40 ℃ and pH 4～6,and the K_m and V_max values were 0.52 mg·ml^-1 and 0.7△E₆₈₀·h^-1,respectively.

Key words: Beauveria bassiana, Chitinase, Solid state fermentation, Kinetic of enzyme reaction

CLC Number:

Q938.1

ZHANG Jie, CAI Jingmin, WU Ke, JIN Shengxian, PAN Renrui, FAN Meizhen . Production and properties of chitinase from Beauveria bassiana Bb174 in solid state fermentation[J]. Chinese Journal of Applied Ecology, 2004, (5): 863-866.

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