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Chinese Journal of Applied Ecology ›› 2004, Vol. ›› Issue (3): 482-486.

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Effect of acetic acid on adsorption of acid phosphatase by some soil colloids and clay minerals

ZHAO Zhenhua1, HUANG Qiaoyun2, JIANG Xin1, YU Guifen1, WANG Fang1, LI Xueyuan 2   

  1. 1. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;
    2. Huazhong Agricultural University, Wuhan 430070, China
  • Received:2002-11-12 Revised:2003-03-31 Online:2004-03-15

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Abstract: This paper studied the effect of acetic acid with different concentrations and pH values on the adsorption of acid phosphatase by some soil colloids and clay minerals (SCCM).The results showed that the pH values for the maximum adsorption of the enzyme were between the IEP of the enzyme and the PZC of SCCM.In the acetic acid systems,the amount of the enzyme adsorbed by SCCM was in the order of goethiteyellow brown soil>latosol>kaolinite>δ-MnO2.A remarkable influence of acetic acid concentration on the adsorption amount and the binding energy of the enzyme was observed.With the increase of the concentration from 0 to 200 mmol·L-1 in the system,acetic acid exhibited an enhanced effect,followed by an inhibition action on the adsorption of the enzyme on SCCM.The changes of the binding energy (K value) for the enzyme on SCCM were on the contrary to those of the maximum adsorption.The possible mechanisms for the influence of acetic acid on the adsorption of enzyme by SCCM were also discussed.

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