Abstract: The behavior ecology of copulation and oviposition of Apis cerana cerana Fab. was studied. The results showed that temperature influence on the cell-covered time of Apis cerana cerana queen and drone induced the remarkable difference in their newborn weight and mature time (P<0.05). Queen and drone fly frequency for discerning cell was 1.23～1.31 and 1.08～1.13,and duration was 0.12～0.13h and 0.16～0.20h. Their copulation fly frequency was 1.10～1.12 and 1.10～1.05,and duration was 0.22～0.23h and 0.18～0.23h. The most suitable temperature for copulation was 20～28℃. The number of semen in side-oviduct of the queen in each copulation fly was 3.37×10⁶～4.15×10⁶,and in natural copulation,semen number in spermathecal sac was 3.55 ×10⁶～3.62×10⁶. There was a direct relationship between queen newborn weight and ovulation number. The annual effect of climate and flower fertility on the queen's ovulation number was obvious.