Effect of Sinorhizobium fredii YC4 symbiotic plasmid amplification on nod factors and symbiotic N-fixation

Abstract

Abstract: Sinorhizobium fredii YC4 can form nitrogen-fixing nodules on soybean (Glycine max) and wild soybean (G.soja).It can produce unique lipochitooligosaccharide nod factors (LCOs), in comparison with the other four strains of S.fredii.The constitution of LCOs produced by YC4 contained more hydrophobic substitutions detected by Thin-layer chromatography (TLC) of ¹⁴C-labeled nod factors.Aspontaneous mutant termed YSC3 amplified in the symbiotic plasmid was isolated from YC4, which can produce more amount of LCOs than its parental strain at 28 ℃,and showed a difference in the construction of LCOs.Nodulation test indicated that YSC3 only formed ineffective nodules on soybean G.soja.

CLC Number:

Q939.113

MIAO Lihong, ZHOU Junchu . Effect of Sinorhizobium fredii YC4 symbiotic plasmid amplification on nod factors and symbiotic N-fixation[J]. Chinese Journal of Applied Ecology, 2003, (9): 1517-1520.

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