Induction of biochemical composition in Heterosigma akashiwo under Fe stress

Abstract

Abstract: The iron-stress mediated effects on biochemical constituents of red tide alga H. akashiwo were examined in Fe-replete and iron deficiency,and low iron batch cultures. The content of all pigments decreased under iron limitation,and the cellular chlorophyll a concentration decreased by more than 2-fold compared to that under iron replete condition. This change in Chl a was accompanied by a corresponding decrease in Chl c per cell, resulting in no trend in the ratio of Chl c to Chl a. The cellular carotenoids content decreased by more than1.5-fold compared to that under iron replete condition. As a consequence, carotenoids/chlorophyll a ratio increased in Fe-deficient cells. Carbohydrate content was reduced under iron stress,and total proteins decreased with the decrease of iron concentration. Acrude fractionation of the soluble proteins demonstrated that 17 kDand 55 kDa proteins in soluble fraction were induced.

CLC Number:

Q178.53

LI Dongxia, CONG Wei, CAI Zhaoling, SHI Dingji, OUYANG Fan . Induction of biochemical composition in Heterosigma akashiwo under Fe stress[J]. Chinese Journal of Applied Ecology, 2003, (7): 1185-1187.

References

[1] Beneová,Niková K,Ferimazová N,et al. 2000. Morphological and physiological differences in Synechococcus elongates during continuous cultivation at high iron, low iron, and iron deficient medium.Photosynthetica,38(2): 233～241
[2] Black EA, Whyte JNC., Bagshaw JW,et al.1991. The effects of Hetersigma akashiwo on juvenile Oncorhynchus tshawytscha and its implications for fish culture.J Appl Ichtyol,7(3): 168～175
[3] Coale KH, Johnson KS, Fitzwater SE,et al. 1996. A massive phytoplankton bloom induced by an ecosystem-scale iron fertilization experiment in the equatorial Pacific Ocean.Nature,383(6600):495～501
[4] Doucette GJ, Harrison PJ.1991. Aspects of iron and nitrogen nutrition in the red tide dinoflagellate Gymnodinium sanguineum. Mar Biol,110(2): 165～173
[5] Dubois M, Gilles KA, Hamilton JK. 1956. Colorimetric method for determination of sugars and related substances.Analyt Chem,28(3): 350～356
[6] Greene RM, Geider RJ, Kolber Z, Flakowski PG. 1992. Iron-induced changes in light harvesting and photochemical energy conversion processes in eukaryotic marine algae.Plant Physiol,100(2): 565～575
[7] Henley WJ, Yin Y. 1998. Growth and photosynthesis of marine Synechococcus (Cyanophyceae) under iron stress.J Phycol,34(1): 94～103
[8] Jeffrey SW, Humphrey GF. 1975. New spectrophotometric equations for determing chlorophylls a, b,c1 and c2 in higher plants, algae and natural phytoplankton. Biochem Physiol Pflanzen,167(2): 191～194
[9] Kim YS, Martin DF. 1974. Interrelationship of Peace River parameter as a basis of the iron index: Predictive guide to the Florida red tide.Water Res,8(9):607～616
[10] La Roche J, Geider RJ, Groziano LM. 1993. Induction of specific proteins in eukaryotic algae grown under iron, phosphorus, or nitrogen-deficient conditions.J Phycol,29(6): 767～777
[11] Laemmli UK. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4.Nature,227(5259): 680～685
[12] Li D-X(李东侠), Cong W(丛威), Cai Z-L(蔡昭铃),et al. 2003. Spectroscopic properties of Heterosigma akashiwo under iron limitation.Chin J Appl Ecol(应用生态学报),14(7):1181～1184(in Chinese)
[13] Li D-X, Cong W, Cai Z-L,et al. 2002. Response of growth and photosynthesis of marine red tide alga Heterosigma akashiwo to iron and iron stress conditions.Biotechnol Lett,24(9):743～747
[14] Markwell MAK, Haas SM, Bieber LL. 1978. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.Anal Biochem,87(1):206～210
[15] Martin JH. Fitzwater SE. 1988. Iron deficiency limits phytoplankton growth in the north-east subarctic Pacific Ocean.Nature,331(6154): 341～343