Effect of short-term phenanthrene stress on SOD activities and MDA contents in soybean (Glycine max) seedlings

Abstract

Abstract: Growth of soybean (Glycine max) seedlings, changes of superoxide dismutase (SOD) activities, and malonyldialdehyde (MDA) contents were researched during different concentration phenanthrene stress and following recovery from stress. The results showed that seedling growth was interrupted after 5d in 200μg·g^-1 phenanthrene treatment. However, during the period of recovery, growth was restored, although there was a delay in returning to control levels.The dose-related response of SODactivities were observed in manner of linear for 2d and of parabola for 5d and 8d at 50～200μg·g^-1 phenanthrene. SODactivities of seedling were significantly induced by 100 and 200μg·g^-1 phenanthrene for 2d (P<0.05 and P<0.01), while declined significantly under phenanthrene stress for 8d, and was 88% of control at 200μg·g^-1 phenanthrene (P<0.05). When releasing soybean seedlings from phenanthrene stress for 5d to clear tap water for 2d or 4d,their SODactivities at 50 and 100μg·g^-1 phenanthrene recovered towards control level, while a significant increase in SOD activities was observed at 200μg·g^-1 phenanthrene (P<0.05). The experiments also revealed that a significant increase of MDA contents in seedlings occurred after 5d and 8d in100 and 200μg·g^-1 phenanthrene treatment (P<0.05 and P<0.01), which suggested that the membrane systems were damaged by phenanthrene stress. All of the above results showed that SOD activities of soybean seedling might be suitable to be the biomarkers of phenanthrene stress.

CLC Number:

X173

LIU Wan, LI Peijun, ZHOU Qixing, SUN Tieheng, TAI Peidong, XU Huaxia, ZHANG Hairong . Effect of short-term phenanthrene stress on SOD activities and MDA contents in soybean (Glycine max) seedlings[J]. Chinese Journal of Applied Ecology, 2003, (4): 581-584.

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