Establishment of trans-inactivation system for haloalkane dehalogenase gene in Arabidopsis thaliana

Abstract

Abstract: With haloalkane dehalogenase gene (dhlA) of Xanthobacter autothophicus as a negative selection marker,a novel trans-inactivation system for transgenes in plants was developed.After the A rabidopsis plants were transformed with dhlA, one plant was shown dhlA post-transcriptional silencing by nuclear run-off transcriptional analysis.The A rabidopsis plants containing silencing locus were crossed to those efficiently expressed dhlA,referred as a homologous transgenic locus,and 96% of F1 plants became the homologous dhlA inactivated in trans. When the F1 plants were selfed,and the silencing locus and the homologous locus were separated in some progenies,42 of the 200 progenies exhibited DhlA activity,and 158 no DhlA activity, and the proportion of the DhlA-expressing plants to the dhlA-silencing ones was 3.76 to 1,which suggested that the silencing locus inactivated in trans the homologous transgenic locus as a Mendelian dominant factor.

CLC Number:

Q943.2

HAO Lin, CAO Jun, FENG Shu . Establishment of trans-inactivation system for haloalkane dehalogenase gene in Arabidopsis thaliana[J]. Chinese Journal of Applied Ecology, 2001, (2): 269-271.

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