Effect of exogenous α-naphthaleneacetic acid on carbon metabolism of soybean under drought stress at flowering stage

doi:10.13287/j.1001-9332.201804.022

Abstract

Abstract: With pot experiment, two soybean (Glycine max) varieties, Jindou 21 (drought-tole-rant) and Xudou 22 (drought-sensitive), were used to examine the effects of α-naphthaleneacetic acid (NAA) on carbon metabolism of soybean under drought stress at flowering stage. The results showed that under drought stress, compared to Xudou 22, Jindou 21 had smaller decrease in net photosynthetic rate (P_n), smaller increase in photorespiration rate (P_r) and soluble sugar content of leaves, while a greater increase in the activities of sucrose phosphate synthase (SPS) and sucrose synthetase (SS) (synthesis) of leaves and sucrose content of roots. NAA treatment increased P_n and decreased P_r under drought stress, and thus obviously alleviated the growth inhibition of drought stress on plants. NAA treatment reduced the activities of starch-degrading enzymes, acid invertase (AI) and SS (cleavage), thereby inhibited the accumulation of soluble sugar induced by drought stress. In addition, NAA treatment increased SPS and SS (synthesis) activities of leaves, sucrose content of roots and shoot-root ratio, indicating that NAA treatment improved the transportation of sucrose from leaf blade to root under drought stress. In conclusion, exogenous NAA could enhance drought tolerance in soybean by regulating carbon metabolism.

XING Xing-hua, XU Ze-jun, QI Yu-jun, WANG Xiao-jun, SUN Dong-lei, BIAN Neng-fei, WANG Xing. Effect of exogenous α-naphthaleneacetic acid on carbon metabolism of soybean under drought stress at flowering stage[J]. Chinese Journal of Applied Ecology, 2018, 29(4): 1215-1224.

References

[1] Jiang H-Q (江洪强), Xing X-H (邢兴华), Zhou Q (周琴), et al. Effects of exogenous α-naphthaleneacetic acid on the antioxidation system in soybean leaves subjected to long-term drought stress during flowering. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(6): 1718-1726 (in Chinese)
[2] Liu T, van Staden J. Partitioning of carbohydrates in salt-sensitive and salt-tolerant soybean callus cultures under salinity stress and its subsequent relief. Plant Growth Regulation, 2001, 33: 13-17
[3] Jing D-W (井大炜), Xing S-J (邢尚军), Du Z-Y (杜振宇), et al. Effects of drought stress on the growth, photosynthetic characteristics, and active oxygen metabolism of poplar seedlings. Chinese Journal of Applied Ecology (应用生态学报), 2013, 24(7): 1809-1816 (in Chinese)
[4] Li Y-Y (李阳阳), Geng Q-Y (耿青云), Fei C (费聪), et al. Physiological responses of sugar beet (Beta vulgaris) to drought stress during vegetative development period under drip irrigation. Chinese Journal of Applied Ecology (应用生态学报), 2016, 27(1): 201-206 (in Chinese)
[5] Du Q-J (杜清洁), Dai K-R (代侃韧), Li J-M (李建明), et al. Effects of sub-low temperature and drought stress on characteristics of photosynthetic and fluorescence kinetics in tomato leaves. Chinese Journal of Applied Ecology (应用生态学报), 2015, 26(6): 1687-1694 (in Chinese)
[6] Zhao W-S (赵文赛), Sun Y-L (孙永林), Liu X-P (刘西平). Effects of drought-rewatering-drought on photosynthesis and growth of maize. Chinese Journal of Plant Ecology (植物生态学报), 2016, 40(6): 594-603 (in Chinese)
[7] Zhao X-Y (赵新宇), Xu K-Z (徐克章),Zhang Y-J (张玉姣), et al. Effect of exogenous hormones on leaf photosynthetic parameters of soybean plants. Soybean Science (大豆科学), 2014, 33(3): 358-364 (in Chinese)
[8] Dong Q-Y (董奇妤), Zhang L (张亮), Zhang H (张亨), et al. Effects of three plant growth regulators on photosynthesis of Evodia glabrifolia. Subtropical Plant Science (亚热带植物科学), 2009, 24(3): 99-101 (in Chinese)
[9] Cui N (崔娜), Li T-L (李天来), Zhao J-Y (赵聚勇), et al. Effects of exogenous auxin on related enzymes activities of sucrose metabolism and gene expression in tomato fruits. Acta Agriculturae Boreali-Sinica (华北农学报), 2009, 24(3): 99-101 (in Chinese)
[10] Tao X (陶霞), Li H-L (李慧琳), Wan L (万林), et al. Alleviation effects of IAA foliar spray on waterlogging stressed rapeseed at budding stage. Chinese Journal of Oil Crop Sciences (中国油料作物学报), 2015, 37(1): 55-61 (in Chinese)
[11] Zhou H (周欢), Wang C-N (王承南), Gu Z-Y (谷战英), et al. Effects of NAA on chlorophyll fluorescence parameters of Euphorbia pulcherrima seedlings under water stress. China Forestry Science and Technology (林业科技开发), 2009, 23(1): 78-80 (in Chinese)
[12] Xing XH, Jiang HQ, Zhou Q, et al. Improved drought tolerance by early IAA- and ABA-dependent H₂O₂ accumulation induced by α-naphthaleneacetic acid in soybean plants. Plant Growth Regulation, 2016, 80: 303-314
[13] Wen G-Q (温国泉), Nong M-L (农梦玲), Liu Y-X (刘永贤), et al. Effect of plant growth regulators (combination) on chlorophyll content and amylolysis of yam. Southwest China Journal of Agricultural Sciences (西南农业学报), 2016, 29(6): 1281-1284 (in Chinese)
[14] Liang P (梁鹏), Xing X-H (邢兴华), Zhou Q (周琴), et al. Effect of NAA on growth and photosynthetic characteristic of soybean seedlings under drought and re-watering. Soybean Science (大豆科学), 2011, 30(1): 50-55 (in Chinese)
[15] Fehr WR, Caviness CE. Stages of Soybean Development. Ames, IW: Iowa State University Cooperative Extension Service Special Report, 1977
[16] Jiang C-D (姜闯道), Gao H-Y (高辉远), Zou Q (邹琦), et al. The co-operation of leaf orientation, photorespiration and thermal dissipation alleviate photoinhibition in young leaves of soybean plants. Acta Ecologica Sinica (生态学报), 2005, 25(2): 319-325 (in Chinese)
[17] Gao J-F (高俊凤). Experimental Guidance for Plant Physiology. Beijing: Higher Education Press, 2006 (in Chinese)
[18] Zhao F-C (赵福成), Jing L-Q (景立权), Lu D-L (陆大雷), et al. Dynamic change of sugar components accumulation and enzyme activity associated with sucrose metabolism during grain filling of super sweet corn. Journal of Nuclear Agricultural Sciences (核农学报), 2014, 28(12): 2230-2237 (in Chinese)
[19] Zhao F-C (赵福成), Jing L-Q (景立权), Yan F-B (闫发宝), et al. Effects of heat stress during grain filling on sugar accumulation and enzyme activity associated with sucrose metabolism in sweet corn. Acta Agronomica Sinica (作物学报), 2013, 39(9): 1644-1651 (in Chinese)
[20] Pavlinova OA, Balakhontsev EN, Prasolova MF, et al. Sucrose-phosphate synthase, sucrose synthase, and invertase in sugar beet leaves. Russian Journal of Plant Physiology, 2002, 49: 68-73
[21] Kato-Noguchi H, Macías FA. Effects of 6-methoxy-2-benzoxazolinone on the germination and alpha-amylase activity in lettuce seeds. Journal of Plant Physioogy, 2005, 162: 1304-1307
[22] Nakamura Y, Yuki K, Park SY, et al. Carbohydrate metabolism in the developing endosperm of rice grains. Plant and Cell Physiology, 1989, 30: 833-839
[23] Li H-S (李合生). Plant Biochemistry Principle and Technique of Experiment. Beijing: Higher Education Press, 2000 (in Chinese)
[24] Wang L (王磊), Zhang T (张彤), Ding S-Y (丁圣彦). Effects of short-term drought and rewatering during the blooming stage on soybean photosynthesis and yield. Chinese Bulletin of Botany (植物学报), 2009, 44(2): 185-190 (in Chinese)
[25] Zhang X-H (张兴华), Gao J (高杰), Du W-L (杜伟莉), et al. Effects of drought stress on photosynthetic characteristics of maize hybrids at seedling stage. Acta Agronomica Sinica (作物学报), 2015, 41(1): 154-159 (in Chinese)
[26] Efeoglu B, Ekmekci Y, Cicek N, et al. Physiological responses of three maize cultivars to drought stress and recovery. South African Journal of Botany, 2009, 75: 34-42
[27] Song X, She X, He J, et al. Cytokinin- and auxin-induced stomatal opening involves a decrease in levels of hydrogen peroxide in guard cells of Vicia faba. Functional Plant Biology, 2006, 33: 573-583
[28] Tetlow IJ, Morell MK, Emes MJ. Recent developments in understanding the regulation of starch metabolism in higher plants. Journal of Experimental Botany, 2004, 55: 2131-2145
[29] Li TH, Li SH. Leaf responses of micropropagated apple plants to water stress: Nonstructural carbohydrate composition and regulatory role of metabolic enzymes. Tree Physiology, 2005, 25: 495-504
[30] Xu Y-C (徐迎春), Li S-H (李绍华), Chai C-L (柴成林), et al. Carbohydrate metabolism in source leaves of jonagold apple tree under water stress and after water relief. Journal of Fruit Science (果树学报), 2001, 18(1): 1-6 (in Chinese)
[31] Praxedes SC, DaMatta FM, Loureiro ME, et al. Effects of long-term soil drought on photosynthesis and carbohydrate metabolism in mature robusta coffee (Coffea canephora Pierre var. kouillou) leaves. Environmental and Experimental Botany, 2006, 56: 263-273
[32] Farrar J, Pollock C, Gallagher J. Sucrose and the integration of metabolism in vascular plants. Plant Science, 2000, 154: 1-11
[33] Chen G-Y (陈国元), Xie P-Y (谢莆尧). Effects of Microcystis aeruginosa stress on photorespiration of Typha latifolia leaves. Chinese Journal of Environmental Engineering (环境工程学报), 2015, 9(4): 1571-1578 (in Chinese)
[34] Sun S-N (孙胜楠), Wang Q (王强), Sun C-C (孙晨晨), et al. Response and adaptation of photosynthesis of cucumber seedlings to high temperature stress. Chinese Journal of Applied Ecology (应用生态学报), 2017, 28(5): 1603-1610 (in Chinese)
[35] Gururani MA, Venkatesh J, Tran LSP. Regulation of photosynthesis during abiotic stress-induced photoinhibition. Molecular Plant, 2015, 8: 1304-1320