Allelopathic effect of extracts from Panax notoginseng mono-cropped soil on its root rot pathogens

doi:10.13287/j.1001-9332.202007.016

Abstract

Abstract: Methanol, ethyl acetate, and water were used to extract the continuous cropping soils of Panax notoginseng, with the solution/soil ratios of 3:1, 6:1, and 9:1. We investigated the effects of those soil extracts on the growth and population of root-rot pathogens of P. notoginseng. Results showed that the methanol, ethyl acetate and water extracts all promoted mycelial growth of Fusarium oxysporum and Fusarium solani after 72 h of plate culture. The response indices of methanol and ethyl acetate extracts on the growth of F. oxysporum were 14.0%-19.8% and 16.2%-20.2%, being higher than that of water extract (8.9%-14.2%), but without significant difference between diffe-rent extraction ratios. However, methanol extract inhibited the mycelial growth of Alternaria spp. The inhibitory effect was highest at the extraction ratio of 3:1, reaching -33.2% to -38.5%. Ethyl acetate and water extracts did not affect the mycelial growth of Alternaria spp. After four weeks of soil culture, methanol, ethyl acetate and water extracts all increased the F. oxysporum populations. The positive effect of water extract was higher than that of methanol (1.68×10⁴-6.73×10⁴ copies·g^-1 dry soil) and ethyl acetate (1.77×10⁴-3.72×10⁴ copies·g^-1 dry soil) extracts, being 3.49×10⁶-9.56×10⁶ copies·g^-1 dry soil. This increment was weakened along with the increase of extraction ratio. Both water extract and methanol extract with low extraction ratio could increase the F. solani populations, while there were no significant effects of methanol, ethyl acetate and water extracts on the population of Alternaria spp. Therefore, the extracts from continuous P. notoginseng cropping soil showed allopathically promoting effects on the growth and population of root-rot pathogens, F. oxysporum and F. solani, which may be one of the reasons for the occurrence of root rot and other soil-borne diseases in replanted P. notoginseng gardens.

Key words: Panax notoginseng, allelopathic effect, Fusarium oxysporum, Fusarium solani, Alternaria spp

YAO Chun-zhi, JIANG Yu-ting, YANG Yu-ting, MA Yan-yu, PAN Lei-lyu, LI Yun-long, SUN Yong-quan, CHEN Ji, ZHAO Jun. Allelopathic effect of extracts from Panax notoginseng mono-cropped soil on its root rot pathogens[J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2227-2235.

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