Kinetic parameters and temperature sensitivity of soil nitrogen and phosphorus transforming enzymes in Robinia pseudoacacia plantations under different vegetation zones on the Loess Plateau, China

doi:10.13287/j.1001-9332.202008.003

Abstract

Abstract: Soil enzymes are catalysts for organic matter decomposition, the kinetic characteristics of which are important indicators of the catalytic performance of enzymes, with important role in evalua-ting soil health quality. We examined the responses of soil enzyme kinetic parameters to temperature change and the variation characteristics of their temperature sensitivity (Q₁₀) in Robinia pseu-doacacia plantation soil under three different vegetation zones on the Loess Plateau. The results showed that the potential maximum reaction rate (V_max) and the half-saturation constant (K_m) of alanine transaminase (ALT), leucine aminopeptidase (LAP) and alkaline phosphatase (ALP) all increased linearly with the increasing incubation temperature. The zonal regularity of forest zone > forest-steppe zone > steppe zone was presented in V_max. The temperature sensitivity of V_max(Q10(V_max)) ranged from 1.14 to 1.62, and the temperature sensitivity of K_m(Q_{10(K_m)}) ranged from 1.05 to 1.47, with both values being lower in forest-steppe zone than other vegetation zones. In low and high temperature regions, the variations of Q₁₀ in different soil enzymes differed among vegetation zones. Results from redundancy analysis showed that Q₁₀ had a significant correlation with environmental variables, especially soil nutrients, indicating that Q₁₀ would be affected by other environmental factors besides temperature.

Key words: Loess Plateau, vegetation zone, soil enzyme kinetic parameter, temperature sensitivity

ZHAO Zi-wen, WANG Guo-liang, WU Yang, CHEN Wen-jing, LI Yang-yang, LIU Guo-bin, XUE Sha. Kinetic parameters and temperature sensitivity of soil nitrogen and phosphorus transforming enzymes in Robinia pseudoacacia plantations under different vegetation zones on the Loess Plateau, China[J]. Chinese Journal of Applied Ecology, 2020, 31(8): 2515-2522.

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