Structure and function of soil bacterial communities in the monoculture and mixed plantation of Pinus massoniana and Castanopsis hystrix in southern subtropical China

doi:10.13287/j.1001-9332.202103.37

Abstract

Abstract: Establishing monoculture of native broadleaved tree species and mixed coniferous broadleaved plantations is the tendency for forest management in subtropical China. The variations of structure and function of soil bacterial community in monoculture and mixed tree plantations are still not clear. We examined soil bacterial community structure and function under different soil layers (0-20, 20-40 and 40-60 cm) in three planted forests, including broadleaved Castanopsis hystrix, coniferous Pinus massoniana and their mixed plantation, in south subtropical China, using 16S rRNA gene high-throughput sequencing and PICRUSt prediction. The results showed that soil bacterial community structure of mixed plantation and P. massoniana plantation were similar but being significant different from that in C. hystrix plantation. The diversity, biological pathways metabolic function, and nitrogen cycling function of soil bacterial community in C. hystrix plantation were lower than those in P. massoniana plantation and mixed plantation. Soil total nitrogen, nitrate nitrogen and C/N were the main factors driving the variations of soil bacterial community structure and function among different forest types. Our results suggested that the mixed plantation of C. hystrix and P. massoniana is better than C. hystrix plantation in this area in terms of soil bacterial community structure and function.

Key words: soil bacterial community, high-throughput sequencing, PICRUSt prediction, nitrogen cycling functional genes, plantation

LIANG Yan, MING An-gang, HE You-jun, LUO Ying-hua, TAN Ling, QIN Lin. Structure and function of soil bacterial communities in the monoculture and mixed plantation of Pinus massoniana and Castanopsis hystrix in southern subtropical China[J]. Chinese Journal of Applied Ecology, 2021, 32(3): 878-886.

References 40

[1]	Nelson MB, Martiny AC, Martiny JBH. Global biogeo-graphy of microbial nitrogen-cycling traits in soil. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113: 8033-8040
[2]	Sun H, Terhonen E, Koskinen K, et al. Bacterial diversity and community structure along different peat soils in boreal forest. Applied Soil Ecology, 2014, 74: 37-45
[3]	Martins CSC, Nazaries L, Macdonald CA, et al. Water availability and abundance of microbial groups are key determinants of greenhouse gas fluxes in a dryland forest ecosystem. Soil Biology & Biochemistry, 2015, 86: 5-16
[4]	Liang S, Deng J, Jiang Y, et al. Functional distribution of bacterial community under different land use patterns based on faprotax function prediction. Polish Journal of Environmental Studies, 2020, 29: 1245-1261
[5]	Bardgett RD, Van Der Putten WH. Belowground biodiversity and ecosystem functioning. Nature, 2014, 515: 505-511
[6]	Bainard LD, Hamel C, Gan Y. Edaphic properties override the influence of crops on the composition of the soil bacterial community in a semiarid agroecosystem. Applied Soil Ecology, 2016, 105: 160-168
[7]	Creamer R, Hannula SE, Van Leeuwen JP, et al. Ecological network analysis reveals the inter-connection between soil biodiversity and ecosystem function as affected by land use across Europe. Applied Soil Ecology, 2016, 97: 112-124
[8]	刘世荣, 杨予静, 王晖. 中国人工林经营发展战略与对策: 从追求木材产量的单一目标经营转向提升生态系统服务质量和效益的多目标经营. 生态学报, 2018, 38(1): 1-10 [Liu S-R, Yang Y-J, Wang H. Development strategy and management countermeasures of planted forests in China: Transforming from timber-centered single objective management towards multi-purpose management for enhancing quality and benefits of ecosystem services. Acta Ecologica Sinica, 2018, 38(1): 1-10]
[9]	Paquette A, Messier C. The role of plantations in mana-ging the world’s forests in the Anthropocene. Frontiers in Ecology and the Environment, 2010, 8: 27-34
[10]	Felton A, Nilsson U, Sonesson J, et al. Replacing mono-cultures with mixed-species stands: Ecosystem service implications of two production forest alternatives in Sweden. Ambio, 2016, 45: 124-139
[11]	Zhou L, Sun Y, Saeed S, et al. The difference of soil properties between pure and mixed Chinese fir (Cunninghamia lanceolata) plantations depends on tree species. Global Ecology and Conservation, 2020, 22: 10.1016/j.gecco.2020.e01009
[12]	颜耀, 张辉, 黄智军, 等. 补植阔叶树种对红壤侵蚀区马尾松林水源涵养功能的影响. 福建农林大学学报, 2020, 49(1): 67-73 [Yan Y, Zhang H, Huang Z-J, et al. Effects of replanting broad-leaved tree species on water conservation of Pinus massoniana plantation in red soil eroded region. Journal of Fujian Agriculture and Forestry University, 2020, 49(1): 67-73]
[13]	温远光, 李海燕, 周晓果, 等. 马尾松×红锥异龄混交林对土壤微生物群落结构和功能的影响. 广西科学, 2019, 26(2): 188-198 [Wen Y-G, Li H-Y, Zhou X-G, et al. Effects of uneven-aged Pinus massonianao × Castanopsis hystrix mixed plantations on structural and functions of soil microbial community. Guangxi Sciences, 2019, 26(2): 188-198]
[14]	谭玲, 何友均, 覃林, 等. 南亚热带红椎、马尾松纯林及其混交林土壤理化性质比较. 西部林业科学, 2014, 43(2): 35-41 [Tan L, He Y-J, Qin L, et al. Comparison of soil physical and chemical properties of pure Castanopsis hystrix, pure Pinus massoniana and mixed-species tree plantation in south subtropical area. Journal of West China Forestry Science, 2014, 43(2): 35-41]
[15]	罗达, 史作民, 唐敬超, 等. 南亚热带乡土树种人工纯林及混交林土壤微生物群落结构. 应用生态学报, 2014, 25(9): 2543-2550 [Luo D, Shi Z-M, Tang J-C, et al. Soil microbial community structure of monoculture and mixed plantation stands of native tree species in south subtropical China. Chinese Journal of Applied Eco-logy, 2014, 25(9): 2543-2550]
[16]	段鹏飞, 陈彦, 张菲, 等. 芒草种植对土壤细菌群落结构和功能的影响. 应用生态学报, 2019, 30(6): 2030-2038 [Duan P-F, Chen Y, Zhang F, et al. Effect of Miscanthus planting on the structure and function of soil bacterial community. Chinese Journal of Applied Ecology, 2019, 30(6): 2030-2038]
[17]	历桂香, 马克明. 北京东灵山树线处土壤细菌的RICRUSt基因预测分析. 生态学报, 2018, 38(6): 2180-2186[Li G-X, Ma K-M. PICRUSt-based predicted meta-genomic analysis of treeline soil bacteria on Mount Dongling, Beijing. Acta Ecologica Sinica, 2018, 38(6): 2180-2186]
[18]	彭雯, 谭玲, 明安刚, 等. 南亚热带典型人工纯林土壤剖面细菌群落组成差异分析. 土壤通报, 2018, 49(6): 1361-1369 [Peng W, Tan L, Ming A-G, et al. Bacterial community composition in soil profile of typical monoculture plantations in south subtropical China. Chinese Journal of Soil Science, 2018, 49(6): 1361-1369]
[19]	Wang H, Liu SR, Mo JM, et al. Soil organic carbon stock and chemical composition in four plantations of indigenous tree species in subtropical China. Ecological Research, 2010, 25: 1071-1079
[20]	Langille MG, Zaneveld J, Caporaso JG, et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nature Biotechnology, 2013, 31: 814-823
[21]	Core R Team. R: A language and environment for statistical computing. Vienna, Austria: R for Statistical Computing, 2014
[22]	Lepš J, Šmilauer P. Multivariate Analysis of Ecological Data Using CANOCO. Cambridge, UK: Cambridge University Press, 2003
[23]	Asnicar F, Weingart G, Tickle TL, et al. Compact graphical representation of phylogenetic data and metadata with GraPhlAn. PeerJ, 2015, 3: 1-17
[24]	Qu Z, Liu B, Ma Y, et al. Differences in bacterial community structure and potential functions among Eucalyptus plantations with different ages and species of trees. Applied Soil Ecology, 2020, 149: 10.1016/j.apsoil.2020.103515
[25]	Ju Y, Zhong R, Christensen MJ, et al. Effects of Epichloe gansuensis endophyte on the root and rhizosphere soil bacteria of Achnatherum inebrians under different moisture conditions. Frontiers in Microbiology, 2020, 11: 401-412
[26]	Gu S, Hu Q, Cheng Y, et al. Application of organic fertilizer improves microbial community diversity and alters microbial network structure in tea (Camellia sinensis) plantation soils. Soil & Tillage Research, 2019, 195: 10.1016/j.still.2019.104356
[27]	Yu P, Sun Y, Huang Z, et al. The effects of ectomycorrhizal fungi on heavy metals’ transport in Pinus massonia- na and bacteria community in rhizosphere soil in mine tailing area. Journal of Hazardous Materials, 2020, 381: 10.1016/j.jhazmat.2019.121203
[28]	邓娇娇, 周永斌, 殷有, 等. 辽东山区典型人工针叶林土壤细菌群落多样性特征. 生态学报, 2019, 39(3): 997-1008 [Deng J-J, Zhou Y-B, Yin Y, et al. Soil bacterial community structure characteristics in coniferous forests of montane regions of eastern Liaoning Province, China. Acta Ecologica Sinica, 2019, 39(3): 997-1008]
[29]	Schneider D, Engelhaupt M, Allen K, et al. Impact of lowland rainforest transformation on diversity and composition of soil prokaryotic communities in Sumatra (Indonesia). Frontiers in Microbiology, 2015, 6: 1339-1339
[30]	Xue L, Ren H, Li S, et al. Soil bacterial community structure and co-occurrence pattern during vegetation restoration in karst rocky desertification area. Frontiers in Microbiology, 2017, 8: 1-11
[31]	陈玉真, 王峰, 吴志丹, 等. 林地转变为茶园对土壤细菌群落结构与多样性的影响. 西北农林科技大学学报, 2020, 48(4): 97-106 [Chen Y-Z, Wang F, Wu Z-D, et al. Effects of forestland to tea garden conversion on soil bacterial community and diversity. Journal of Northwest A&F University, 2020, 48(4): 97-106]
[32]	Wu S-J, Deng J-J, Yin Y, et al. Bacterial community changes associated with land use type in the forest montane region of northeast China. Forests, 2020, 11: 10.3390/f11010040
[33]	Ahamed Bakeri S. Soil bacterial biodiversity in development of secondary loggedover forest to oil palm plantation in mineral soil of belaga, sarawak. Journal of Oil Palm Research, 2019, 31: 394-411
[34]	Tolli JD, King GM. Diversity and structure of bacterial chemolithotrophic communities in pine forest and agroeco-system soils. Applied and Environmental Microbiology, 2005, 71: 8411-8418
[35]	Garau G, Morillas L, Roales J, et al. Effect of monospecific and mixed Mediterranean tree plantations on soil microbial community and biochemical functioning. Applied Soil Ecology, 2019, 140: 78-88
[36]	杜宇佳, 高广磊, 陈丽华, 等. 呼伦贝尔沙区土壤细菌群落结构与功能预测. 中国环境科学, 2019, 39(11): 4840-4848 [Du Y-J, Gao G-L, Chen L-H, et al, Soil bacteria community structure and function prediction in the Hulun Buir Sandy Area. China Environmental Science, 2019, 39(11): 4840-4848]
[37]	Kuypers MMM, Marchant HK, Kartal B. The microbial nitrogen-cycling network. Nature Reviews Microbiology, 2018, 16: 263-276
[38]	董志颖, 洪慢, 胡晗静, 等. 过量氮输入对寡营养海水细菌群落代谢潜力的影响. 环境科学学报, 2018, 38(2): 457-466 [Dong Z-Y, Hong M, Hu H-J, et al. Effect of excess nitrogen loading on the metabolic potential of the bacterial community in oligotrophic coastal water. Acta Scientiae Circumstantiae, 2018, 38(2): 457-466]
[39]	Sun HW, Shi WY, Cai CJ, et al. Responses of microbial structures, functions, metabolic pathways and community interactions to different C/N ratios in aerobic nitrification. Bioresource Technology, 2020, 311: 10.1016/j.biortech.2020.123422
[40]	张拓, 徐飞, 怀宝东, 等. 松花江下游沿江湿地土地利用变化对土壤细菌群落多样性的影响. 环境科学, 2020, 41(9): 4273-4283 [Zhang K, Xu F, Huai B-D, et al. Effects of land use changes on soil bacterial community diversity in the riparian wetland along the downstream of Songhua River. Environmental Science, 2020, 41(9): 4273-4283]