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    18 August 2013, Volume 24 Issue 8
    Evapotranspiration characteristics of artificial and natural forests in Liupan Mountains of Ningxia, China during growth season.
    CAO Gong-xiang1, WANG Xu-fang2, XIONG Wei1, WANG Yan-hui1, YU Peng-tao1, WANG Yun-ni1, XU Li-hong1, LI Zhen-hua1
    2013, 24(8):  2089-2096. 
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    In order to understand the effects of the structure of forest ecosystem on the hydrological processes, a comparative study by using thermal dissipation technique and hydrological methodology was made on the evapotranspiration (ET) and its components of Larix principisrupprechtii plantation and Pinus armandi natural forest in two adjacent stands in a small catchment Xiangshuihe of Liupan Mountains during the growth season (May-October) in 2009. Throughout the growth season, the total ET from the plantation was 518.2 mm, which accounted for 104.6% of the precipitation and was much higher than that (420.5 mm) of the natural forest. The allocation of ET in the vertical layers performed similarly between the two stands, with the order of canopy layer > herb and soil layer > shrub layer, but the ratio of each component to total ET differed significantly. The plantation consumed 0.2 and 0.9 times more water for canopy interception (19.6 mm per month) and tree transpiration (25.2 mm per month) than the natural forest, respectively. However, the transpiration from the plantation was 4.4 mm per month, and took up 23.4% of the natural forest. In contrast, the sum of soil evaporation and herbage evapotranspiration consumed 37.1 mm water per month in the plantation, which was 0.8 times higher than that in the natural forest. The ET was calculated by Penman-Monteith equation to compare the results estimated by sap flow measurements, and the values estimated by the two methods were similar.
    Effects of forest gap size and uprooted microsite on the microclimate in Pinus koraiensisdominated broad-leaved mixed forest.
    DUAN Wen-biao, DU Shan, CHEN Li-xin, WANG Li-xia, WEI Quan-shuai, ZHAO Jian-hui
    2013, 24(8):  2097-2105. 
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    Three representative forest gaps with pit-mound microsites formed by uprooted trees were selected within the 2.55 hm2  plot in a Pinus koraiensisdominated broadleaved mixed forest in Xiao Xing’an Mountains of Northeast China. The cleared land and closed stand were set up as the controls, and the PAR, air temperature and relative humidity in the centers of different size gaps and in mound top as well as the total radiation and precipitation in the gap centers were measured between July and September, 2011 by using multichannel automatic meteorological stations. The differences of the microclimate between the gap centers and mound top in different months were compared, and the monthly and diurnal variations of the microclimatic factors in the gap centers and in the mound top under typical weather conditions were analyzed. The results showed that the mean monthly PAR and air temperature in the three gaps of different sizes were in the order of large gap > medium gap > small gap, and the mean monthly relative humidity was in the order of small gap > medium gap > large gap. For the same size gap, the mean monthly PAR and air temperature were higher in the mound top than in the gap center, whereas the mean monthly relative humidity was higher in the gap center than in the mound top. Both the mean monthly total radiation and the mean monthly air temperature in the forest gaps and in the controls were in the order of July > August > September and of cleared land > large gap > medium gap > small gap > closed stand, while the mean monthly relative humidity was in the order of closed stand > small gap > medium gap > large gap > cleared land. The differences in the mean monthly relative humidity between closed stand and various gaps and between closed stand and cleared land reached significant level. The monthly precipitation from July to September decreased in the order of cleared land > large gap > medium gap > small gap > closed stand. Whether in sunny days or in overcast days, the mean daily PAR and air temperature were higher in mound top than in gap center, and the mean daily relative humidity was in opposite. Whether in mound top or in gap center, the mean daily PAR and air temperature were higher in sunny days than in overcast days, while the mean daily relative humidity was higher in overcast days than in sunny days.
    Seedling regeneration and  affecting factors of Quercus variabilis in  different distribution regions.
    WU Min, ZHANG Wen-hui, ZHOU Jian-yun, MA Chuang, MA Li-wei
    2013, 24(8):  2106-2114. 
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    Twenty four fixed plots in three distribution regions of Quercus variabilis (Loess Plateau, marginal distribution zone; north  slope of Qinling Mountains, semiarid core area; and south slope of Qinling Mountains, moist core area) were installed, respectively, to investigate the age structure, growth status, and dry mass accumulation and allocation of 1-8 years old Q. variabilis seedlings, and path analysis was adopted to determine the key factors affecting the regeneration of the seedlings. In the distribution regions, the density of the seedlings decreased with their increasing age, and the density of the 1-8 years old seedlings all decreased in the order of south slope of Qinling Mountains > north slope of Qinling Mountains > Loess Plateau. The transformation rate of the seedlings with adjacent ages differed significantly among the three distribution regions. On Loess Plateau, the transformation rate of 7 years old to 8 years old seedlings was the lowest (30.2±2.9) %; on the north and south slopes of Qinling Mountains, the transformation rate of 4 years old to 5 years old seedlings was the lowest, being (53.9±3.7) % and (50.0±2.1) %, respectively. With the increasing age of the seedlings, their height and dry mass presented an increasing trend, with the order of south slope of Qinling Mountains > north slope of Qinling Mountains > Loess Plateau, the rate of root length to plant height tended to decline, and the rates of root breadth to canopy breadth and of root dry mass to shoot dry mass decreased after an initial increase. The rates of root length to plant height, root breadth to canopy breadth, and root dry mass to shoot dry mass were all the highest on Loess Plateau, and the lowest on south slope of Qinling Mountains. Air temperature, irradiance, canopy density and shrub coverage were the direct key factors affecting Q. variabilis seedling regeneration, among which, air temperature and irradiance were the positive factors, while canopy density and shrub coverage were the negative ones. Soil available nitrogen content and herb coverage were the indirect key factors affecting the Q. variabilis seedling regeneration positively and negatively, respectively.
    Population structure and spatial distribution pattern of Camellia azalea in E’huangzhang Nature Reserve of Guangdong, China.
    LI Xin-lei1,2, SUN Zhen-yuan2, LI Ji-yuan1, XU Yi3, LUO Jian3
    2013, 24(8):  2115-2121. 
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    This paper studied the structures of basal diameter,  height  and canopy width  of Camellia azalea population in E’huangzhang Nature Reserve of Guangdong. The spatial distribution patterns and dynamics of the population were measured by applying aggregate indices including disperse coefficient, negative binomial distribution, Cassie index, clumping index, mean crowding, patch index and Green index. The results showed that in the natural distribution region, the population was mainly composed of adult trees, and was in declining due to seriously lack of seedlings. The structures of diameter, height  and canopy width  were not identical among different plots. In the plots, the spatial distribution pattern of C. azalea population showed clump or random, and changed from clump to random with the development of the population.
    Impacts of Ochotona pallasi disturbance on alpine grassland community characteristics.
    ZHAO Guo-qin1, LI Guang-yong1, MA Wen-hu2, ZHAO Dian-zhi3, LI Xiao-yan1,4
    2013, 24(8):  2122-2128. 
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    Plateau pika is the main fossorial mammal in the alpine grassland in Qinghai Lake Watershed of Northwest China. Based on the field investigation data from 18 alpine grassland quadrats in the Watershed, and by using redundancy analysis (RDA) and the surface fitting offered by ‘RVegan’, the disturbance intensity of plateau pika (Ochotona pallasi) was classified as four levels. In order to explore the impacts of plateau pika disturbance on the alpine grassland ecosystem and its grazing quality, the community characteristics under different disturbance intensities by plateau pika were analyzed, and a conceptual model about the alpine grassland community succession was proposed. The results showed that with the increase of the disturbance intensity, the dominant species changed from Juncus roemerianus to Poa pratensis and Laux maritima. When the disturbance was small, the community had high quantitative values of coverage, aboveground biomass, biodiversity, and species richness, but the proportion of weeds was also high. When the disturbance was large, the quantitative values were the lowest, while the proportion of weeds was the highest. When the disturbance was moderate, the community had relatively high quantitative values, and the proportion of grasses and sedges was the highest. It was concluded that the community’s characteristic values under low plateau pika disturbance intensity were high but the grazing quality was low, while high disturbance intensity resulted in the grassland degradation. Therefore, the disturbance intensity in the threshold could maintain the stability of alpine grassland ecosystem and improve its grazing quality.
    Biomass and carbon storage of Phragmites australis and Spartina alterniflora in Jiuduan Shoal Wetland of Yangtze Estuary, East China.
    LIU Yu1, LI Xiu-zhen1, YAN Zhong-zheng1, CHEN Xiu-zhi2, HE Yan-long1, Guo Wen-yong1, SUN Pei-ying1
    2013, 24(8):  2129-2134. 
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    By the methods of field survey and laboratory analysis, an investigation was conducted on the seasonal dynamics of biomass and carbon storage of Phragmites australis and Spartina alterniflora dominated vegetation belts in the Jiuduan Shoal Wetland of Yangtze Estuary, East China in 2010-2012. The organic carbon storage of the biomass (including aboveground part, underground part, and standing litter) of the two plants was the highest in autumn and the lowest in spring. The average carbon storage of the biomass of S. alterniflora per unit area (445.81 g·m-2) was much higher than that of P. australis (285.52 g·m-2), and the average carbon storage of the standing litter of S. alterniflora (315.28 g·m-2) was also higher than that of P. australis (203.15 g·m-2). However, the organic carbon storage in the surface soil (0-30 cm) under P. australis community (1048.62 g·m-2) was almost as twice times as that under S. alterniflora community (583.33 g·m-2). Overall, the carbon accumulation ability of P. australis community (3212.96 g·m-2) was stronger than that of the S. alterniflora community (2730.42 g·m-2). Therefore, it is of significance to protect the P. australis community in terms of carbon sequestration at the salt marsh.
    Variations of soil respiration flux components in a Larix gmelinii plantation during growth season.
    MENG Chun1, LUO Jing1, PANG Feng-yan2
    2013, 24(8):  2135-2140. 
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    By using trenchingbox method and Li-8150, an observation on the diurnal and monthly variations of soil respiration flux components in a Larix gmelinii plantation in the Harbin Experimental Forest Farm of Northeast Forestry University was conducted in 2010, with the temperature sensitivity of the flux components analyzed. The diurnal variations of the respiration flux of litter, root and mineral soil presented a single peak pattern. From May to October, the diurnal variation amplitudes of the respiration flux of litter, root and mineral soil were 3.1%-12.4%, 1.9%-8.7%, and 10.9%-67.2%, respectively. The mean values of the respiration flux of litter and root occupied 21.2%, 11.1%, 13.4%, 12.0% and 14.2%, and 10.3%, 8.8%,11.6%, 10.0% and 12.5% of the total, with a small diurnal fluctuation and the mean monthly value being 14.3% and 10.6%, respectively. The mean value of the respiration flux of mineral soil was 68.5%, 802%, 75.1%, 78.1% and 73.3%, also with a small diurnal fluctuation and the mean monthly value being 71.5%. The sensitivity of the respiration flux of litter and mineral soil to the temperature at 10 cm soil depth was significantly higher than that to the temperature at soil surface, and the Q10 value of the respiration flux of mineral soil was higher than that of the respiration flux of litter. No significant difference was observed in the sensitivity of the respiration flux of root to the temperature at soil surface and at 10 cm soil depth. The monthly variation of the Q10 for the respiration flux of litter and root was higher at low temperature and lower at high temperature. On the contrary, the monthly variation of the Q10 for the respiration flux of mineral soil was lower in summer and higher in spring and autumn.
    Effects of water table manipulation on the soil respiration in a reclaimed tidal wetland at Dongtan of Chongming Island, China.
    ZHONG Qi-cheng, GUAN Yue-zhang, LIU Qian, CAO Liu-fang, LU Ying, WANG Lu, WANG Kai-yun
    2013, 24(8):  2141-2150. 
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    From January 2011 to January 2012, a monitoring was made on the soil respiration rate and its major affecting environmental factors along a gradient of water table (low, medium and high) in a reclaimed tidal wetland at the Dongtan of Chongming Island in the Yangtze Estuary of China. The annual soil respiration rate in the wetland with low, medium and high water table was 0.75-11.57, 0.70-12.61, and 0.83-6.67 μmol·m-2·s-1, respectively. The soil respiration rate was the maximum in July and the minimum in January. The soil temperature in 0-5 cm layer was the key microclimate factor driving the soil respiration across the three gradients, which could explain more than 70% of the seasonal variation of soil respiration in the reclaimed tidal wetland by fitting an exponential model. No significant difference was observed in the temperature sensitivity of soil respiration (Q10 value) among the three gradients. The lowest soil respiration rate in the wetland with high water table was probably due to the lower soil temperature and the higher soil volumetric water content, whereas the higher soil respiration rate in the wetland with medium water table than with low water table could be caused by the lower soil electricity conductivity and bulk density and the higher aboveground biomass and live fine root density. To appropriately manipulate the water table in the reclaimed tidal wetland could decrease soil respiration rate and enhance the carbon sink function of this degraded wetland.
    Effects of artificial vegetation on the spatial heterogeneity of soil moisture and salt in coastal saline land of Chongming Dongtan, Shanghai.
    HE Bin, CAI Yong-li, RAN Wen-rui, ZHAO Xiao-lei
    2013, 24(8):  2151-2158. 
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    By the methods of classical statistics and geostatistics, this paper studied the spatial heterogeneity of surface soil (0-20 cm layer) moisture and salt contents under three kinds of artificial vegetation in coastal salt land in Chongming Dongtan of Shanghai. The soil moisture content in different  plots was in order of Cynodon dactylon > Taxodium distichum > Nerium indicum, and the coefficient of variation was 13.9%, 13.4% and 12.9%, respectively. The soil electric conductivity was in the order of N. indicum > C. dactylon > T. distichum, and the coefficient of variation was 79.2%, 55.4% and 15.9%, respectively. Both the soil moisture content and the salt content  were in moderate variation. The theoretical models of variogram for the soil moisture and salt contents in different  plots varied, among which, the soil electric conductivity fitted better, with R2 between 0.97 and 0.99. When the artificial vegetation varied from N. indicum to T. distichum and then to C. dactylon, the spatial heterogeneity of soil moisture content changed from weak to strong, in which, the variability was random under N. indicum. When the vegetation varied from C. dactylon to T. distichum and to N. indicum, the spatial heterogeneity of soil electric conductivity changed from moderate to strong. Under different vegetations, the soil
    electric conductivity was mostly in positive correlation, whereas the soil moisture content was in negative correlation. The spatial pattern of soil moisture and salt contents under T. distichum was in striped distribution, that under C. dactylon was in large plaque and continuous distribution, whereas under N. indicum, the spatial pattern of soil moisture content was in small breaking plaque distribution, and that of soil salt content was in striped distribution.
    Assessment of soil nutrient status in urban green space of main cities in Hubei Province, China.
    LI Zhi-guo1, ZHANG Guo-shi1, LIU Yi1, WAN Kai-yuan1, ZHANG Run-hua2, CHEN Fang1
    2013, 24(8):  2159-2165. 
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    According to the topography of the cities in Hubei Province, soil samples were collected from the urban green space in two mountainous cities (Enshi and Shiyan), three hilly cities (Jingmen, Xiangfan and Yichang), and five plain cities (Wuhan, Xiaogan, Xianning, Jingzhou, Suizhou and Huangshi). Within each city, subsoil samples were taken in accordance with four different types of land use, including park, residential, institutional (school, hospital and government, etc.), and roadside. In the main cities in Hubei, the soil pH of urban green space was averagely 7.9, being obviously higher than that of natural soils, while the soil organic matter content was rather low (6.8 g·kg-1). The soil available N and P contents were at a low level, while the soil available trace element (Ca, Mg, S, Fe, Cu, Mn, Zn and B) contents were moderate. Land use type had significant effects on the soil nutrient contents in plain cities. The soil pH in the residential green space was significantly higher than that in the park, roadside and institutional green space, while the contents of soil available trace elements (S, Cu, Mn and Zn) in roadside green space were significantly higher than those of green space in the other land use types. Park green space had the lowest soil nutrient contents. There existed significant differences in the soil nutrient contents among the cities with different topography. The soil organic matter, NH4--N, available K and P, and Ca, Mg, S, Fe, Cu and Mn contents were significantly higher in plain cities than in mountainous cities.
     
    Effects of biochar application on greenhouse gas emission from paddy soil and its physical and chemical properties.
    LIU Yu-xue1,2, WANG Yao-feng1,3, LU Hao-hao1,2, CHEN Yi1,2, TANG Xu1,2, WU Chun-yan1,2, ZHONG Zhe-ke2,4, YANG Sheng-mao1,2
    2013, 24(8):  2166-2172. 
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    A field experiment was conducted to investigate the effects of rice straw returning and rice straw biochar and life rubbish biochar application on the greenhouse gas (CH4, CO2 and N2O) emission from paddy soil, its physical and chemical properties, and rice grain yield.  Compared with rice straw returning, applying rice straw biochar decreased the cumulative CH4 and N2O emissions from paddy soil significantly by 64.2%-78.5% and 16.3%-18.4%, respectively. Whether planting rice or not, the cumulative N2O emission from paddy soil under the applications of rice straw biochar and life rubbish biochar was decreased significantly,  compared with that without biochar amendment. Under the condition of no rice planting, applying life rubbish biochar reduced the cumulative CO2 emission significantly by 25.3%. Rice straw biochar was superior to life rubbish biochar in improving soil pH and available potassium content. Both rice straw biochar and life rubbish biochar could increase the soil organic carbon content significantly, but had less effects on the soil bulk density, total nitrogen and available phosphorus contents, cation exchange capacity (CEC), and grain yield. It was suggested that  compared with rice straw returning, straw biochar was more effective in improving rice grain yield.
    Effects of herbivorous insect stress on the growth and yieldrelated traits of insectresistant transgenic rice.
    ZHANG Fu-li1, LEI Shao-rong1, LIU Yong1,2, SONG Jun1, NIU Bei3, YIN Quan1, DAI Xiao-hang1
    2013, 24(8):  2173-2178. 
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    A field experiment was conducted to test the effects of high and low herbivorous insect stresses on the growth, development and yieldrelated traits of insectresistant transgenic rice. Three Bt transgenic rice Bt63, R1 and R2 were selected as the test materials, and nontransgenic rice Shanyou63 was taken as the control. The differences in the vegetative growth, seedsetting, and stem borer resistance between transgenic and nontransgenic rice were compared. Under herbivorous insect stress, the transgenic rice fully displayed the stem borerresistance of exogenous gene. Under the high stress, the stem borerdamaged degree of the three transgenic rice lines was much lower than that of the control. The plant height, tillers per plant, aboveground fresh mass, panicle length, panicle fresh mass, productive panicles per plant, filled grains per plant, grain mass per plant, seed setting rate, and 1000grain mass of the three transgenic rice lines excelled the control, but had no significant differences except plant height, tillers per plant, and panicle length. Therefore, introducing exogenous Bt gene into rice had no negative effect on rice seedsetting, and high herbivorous insect stress had less impacts on transgenic rice yield.
    Effects of Fe-Cd interaction on the lipid peroxidation and antioxidative enzyme activities of rice.
    LIU Hou-jun1, LI Xue-ping1, HAN Xiao-ri1,2, LIU Yi-fei1, LU Jun-jun1
    2013, 24(8):  2179-2185. 
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    Taking rice variety Shennong 265 as test material, a hydroponic experiment was conducted to investigate the effects of Fe (0, 0.1, 0.25 and 0.5 mmol Fe2+·L-1) and Cd (0, 0.1 and 1.0 umol  Cd2+·L-1) on the lipid peroxidation and antioxidative enzyme activities of rice plant. When the Fe was supplied alone, the shoot and root dry mass decreased significantly, but this phenomenon would not occur when the Cd was applied simultaneously. Applying Cd alone decreased the root malondialdehyde (MDA) and soluble protein contents, but applying Fe simultaneously alleviated the negative effects of Cd. Applying Fe decreased the Cd concentrations in shoots and roots, whereas applying Cd decreased the shoot and root Fe concentrations, indicating an obvious antagonistic interaction between Fe and Cd. The interaction of high concentration (1.0 μmol·L-1) Cd with Fe increased the root MDA and soluble protein contents, and decreased the root superoxide dismutase (SOD) and catalase (CAT) activities. These results indicated that applying definite amount of exogenous Fe could decrease the Cd accumulation in rice under low Cd stress, whereas high Cd stress would decrease the Fe absorption by rice and induce the lipid peroxidation in rice plant.
    Effects of irrigation with different length micro-sprinkling hoses on soil water distribution, water consumption characteristics of winter wheat, and its grain yield.
    MAN Jian-guo, WANG Dong, YU Zhen-wen, ZHANG Yong-li, SHI Yu
    2013, 24(8):  2186-2196. 
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    Taking the high-yielding winter wheat variety Jimai 22 as test material, a field experiment was conducted in 2010—2012 to study the effects of irrigation with different length microsprinkling hoses on the soil water distribution in winter wheat growth period and the water consumption characteristics and grain yield of winter wheat. Three microsprinkling hose lengths were designed, i.e., 40 m (T40), 60 m (T60) and 80 m (T80). Under the microsprinkling irrigation at jointing  and anthesis stages, the uniformity of the horizontal distribution of irrigation water in soil increased significantly with the decrease of hose length from 80 to 40 m. When irrigated at jointing stage, the water content of 0-200 cm soil layer in each space of wheat rows had no significant difference within the 0-40 m distanced from the border initial in treatments T40 and T60. When measured at the 38-40 m, 58-60 m, and 78-80 m distanced from the border initial in treatment T80 at jointing and anthesis stages, the water content in 0-200 cm soil layer had the same change pattern, i.e., decreased with the increasing distance from microsprinkling hose. The water consumption amounts in 40-60 cm soil layer from jointing
     to anthesis stages and in 20-80 cm soil layer from anthesis to maturing stages were higher in treatment T40 than in treatments T60 and T80. However, the soil water consumption amount, irrigation amount at anthesis stage, total irrigation amount, and total water consumption amount were significantly lower in treatment T40 than in treatments T60 and T80. The grain yield, yield water use efficiency increased with the hose length decreased from 80 to 40 m, but the flow decreased. Therefore, the effective irrigation area per unit time decreased with the same irrigation amounts. Considering the grain yield, water use efficiency, and the flow through micro-sprinkling hose, 40 and 60 m were considered to be the appropriate microsprinkling hose lengths under this experimental condition.
    Responses of winter wheat tillers at different positions to low temperature stress at stem elongation stage and their freezing resistance evaluation.
    XU Hai-cheng, YIN Yan-ping, CAI Tie, NI Ying-li, YANG Wei-bing, PENG Dian-liang, YANG Dong-qing, WANG Zhen-lin
    2013, 24(8):  2197-2204. 
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    Taking two winter wheat cultivars Ji’nan 17 and Shannong 8355 as test materials, this paper measured the superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities and the malondialdehyde (MDA) and soluble protein contents in the functional leaves and sheaths of the tillers at different positions at stem elongation stage under low temperature stress, and then, the freezing resistance of the tillers was comprehensively evaluated by the methods of principal component analysis and cluster analysis. The results showed that under low temperature stress, the SOD, POD, and CAT activities in the functional leaves and sheaths of each tiller at stem elongation stage increased, but the MDA and soluble protein contents increased or decreased to some extent. By using principal component analysis and cluster analysis, the tillers of each cultivar were grouped into three kinds of freezing resistance type. For Ji’nan 17, the main stem, tiller Ⅰ, and tiller Ⅱ belonged to high freezing resistance type, the tiller Ⅲ, tiller Ⅳ, and tiller Ⅰp belonged to medium freezing resistance type, and the tiller Ⅱ p belonged to low freezing resistance type. For Shannong 8355, the main stem, tiller Ⅰ, tiller Ⅱ, and tiller Ⅲ belonged to high freezing resistance type, the tiller Ⅳ and tiller Ⅰp belonged to medium freezing resistance type, and the tiller Ⅱp belonged to low freezing resistance type. It was concluded that the freezing resistance of the winter wheat tillers at different positions at stem elongation stage differed, with the lower position tillers being more resistant than the higher position tillers.
    Effects of sprinkler irrigation on the plant nitrogen accumulation and translocation and kernel protein content of winter wheat.
    YAO Su-mei1, KANG Yue-hu2, RU Zhen-gang1, LIU Ming-jiu1, YANG Wen-ping1,LI Gan1
    2013, 24(8):  2205-2210. 
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    Taking wheat cultivar Bainong AK58 as test material, a field experiment was conducted to study the plant nitrogen accumulation and translocation and kernel protein content of winter wheat under sprinkler irrigation and surface irrigation, aimed to understand the differences in the nitrogen metabolism characteristics of winter wheat under different irrigation regimes. At booting stage, no significant difference was observed in the total amount of plant nitrogen accumulation between sprinkler irrigation and surface irrigation; while from booting stage to maturing stage, the total amount of plant nitrogen accumulation under sprinkler irrigation was significantly higher. Under sprinkler irrigation, the translocation amount and contribution rate of the nitrogen stored in leaf, glume, stem and sheath at pre-anthesis to the kernel increased, while the contribution rate of the assimilated nitrogen after anthesis to the kernel nitrogen declined. Both the relative protein content and the total protein yield in the kernel increased significantly under sprinkler irrigation. In conclusion, sprinkler irrigation could significantly regulate the nitrogen translocation and kernel protein accumulation of winter wheat.
    Effects of tillage mode on water use efficiency and yield of summer maize under different simulated rainfalls.
    CHEN Yu, WEN Xiao-xia, LIAO Yun-cheng
    2013, 24(8):  2211-2221. 
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    Based on the tillage practices of summer maize and the rainfall pattern in Northwest China, and by using self-made simulated rainfall device, a field experiment was conducted on the effects of plowing, no-tillage, and no-tillage plus mulching on the water use efficiency (WUE) and yield of summer maize under rainfalls 250, 350 and 450 mm from June to September, 2010.  Compared with plowing, no-tillage increased the WUE and yield under rainfall 250 mm by 26% and 16.5% and under rainfall 350 mm by 17.6% and 6.1%, respectively. Under rainfall 450 mm, the water storage was smaller in treatment no-tillage than in treatment plowing, and the WUE and yield in treatment no-tillage were 1.1% and 0.6% lower than those in treatment plowing, respectively. No-tillage plus mulching overcame the disadvantage of no-tillage in lesser water-storing under sufficient rainfall than plowing. Under the three rainfalls, no-tillage plus mulching could effectively inhibit the soil evaporation between plants, decrease the invalid water consumption of bare soil, and increase the soil water storage and the rate of evapotranspiration to water consumption.  Compared with plowing, no-tillage plus mulching increased the WUE and yield under rainfall 250 mm by 48.6% and 32.9%, under rainfall 350 mm by 51.6% and 27.1%, and under 450 mm rainfall by 23.7% and 13.1%, respectively. In sum, relative to plowing, notillage showed its superiority in increasing WUE and yield under rainfalls 250  and 350 mm, whereas no-tillage plus mulching increased the WUE and yield significantly under rainfalls 250 and 450 mm.
    Coupling effect of water and nitrogen on spring maize in Wuwei Oasis of Shiyang River Basin, Northwest China.
    LIU Xiao-gang1,2, ZHANG Fu-cang2, YANG Qi-liang1, TIAN Yu-feng2, LI Zhi-jun2
    2013, 24(8):  2222-2228. 
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    To explore the optimal supply model of water and nitrogen for spring maize under limited irrigation in arid Northwest China, a field experiment with orthogonal design was conducted in the Wuwei Oasis region margin of Shiyang River Basin to study the effects of irrigation amount at different growth stages and the nitrogen application rate on the group yield and the water and nitrogen utilization of spring maize. With the increase of nitrogen application rate, the grain yield of spring maize increased, and the highest grain yield was obtained when the nitrogen application rate was 300 kg·hm-2 and the irrigation amount at jointing stage was 136 mm. The grain irrigation water use efficiency (GIWUE) decreased with increasing irrigation amount. When the irrigation amount in whole growth period was 340 mm, the grain yield and GIWUE were improved simultaneously with increasing nitrogen application rate. The GIWUE reached the maximum when the nitrogen application rate was 300 kg·hm-2 and the irrigation amount at  seedling and grainfilling stages was 34 mm, respectively. The effects of nitrogen application and irrigation on the nitrogen accumulation in the whole plant decreased in the order of nitrogen application rate, irrigation at jointing stage, irrigation at seedling stage, irrigation at grainfilling stage, and irrigation at heading stage. The optimal supply model of water and nitrogen for spring maize in Wuwei Oasis was 300 kg·hm-2 of nitrogen application plus 34, 136, 68 and 102 mm of irrigation at seedling, jointing, heading and grain-filling stages, respectively.
    Effects of fruit bag color on the microenvironment, yield and quality of tomato fruits.
    WANG Lei1,2, GAO Fang-sheng3, XU Kun1, XU Ning1
    2013, 24(8):  2229-2234. 
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    In order to clarify the ecological and biological effects of fruit bagging, tomato variety JYK was taken as the test material to study the changes of the microenvironment in different color fruit bags and the effects of these changes on the fruit development, yield and quality, with the treatment without fruit bagging as the control (CK). The results showed that bagging with different color fruit bags had positive effects in decreasing the light intensity of the microenvironment and increasing its temperature and humidity, and thus, increased the single fruit mass and promoted the harvest stage advanced. Black bag had the best effects in increasing microenvironment temperature and fruit mass, with the single fruit mass increased by 27.2% and the harvest period shortened by 10 days,  compared with CK. The fruit maturation period in colorless bag, blue bag and red bag was shortened by 8, 3 and 2 days, and the single mass was increased by 11.8%, 6.4% and 4.8%, respectively. Moreover, the coloring and lycopene content of the fruits with different color bags bagging were improved, but the fruit rigidity and fruit soluble solid, soluble protein, and soluble sugar contents were decreased. Therefore, bagging with different color bags could improve the yield of tomato fruits, but decrease the fruit nutritional quality.
    Effects of exogenous nitric oxide on physiological characteristics of longan (Dimocarpus longana) seedlings under acid rain stress.
    LIU Jian-fu, WANG Ming-yuan, YANG Chen, ZHU Ai-jun
    2013, 24(8):  2235-2240. 
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    This paper studied the effects of exogenous nitric oxide donor sodium nitroprusside (SNP) on the chlorophyll content, antioxidant enzyme activities, and osmotic regulation substances of longan (Dimocarpus longana ‘Fuyan’) seedlings under acid rain (pH 3.0) stress. Under the acid rain stress, the seedling leaf superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities and  chlorophyll, soluble protein and soluble sugar contents decreased obviously, while the leaf malondialdedyde content had a remarkable increase, suggesting the toxic effect of the acid rain on the seedlings. Exogenous nitric oxide had dual nature on the physiological characteristics of longan seedlings under acid rain stress. Applying 0.1-0.5 mmol·L-1 of SNP improved the SOD, POD and CAT activities and the  chlorophyll, soluble protein and soluble sugar contents significantly, and decreased the malondialdedyde content. Low concentrations SNP reduced the oxidative damage caused by the acid rain stress, and 0.5 mmol·L-1 of SNP had the best effect. Under the application of 0.5 mmol·L-1 of SNP, the total chlorophyll, soluble protein, and soluble sugar contents and the SOD, POD and CAT activities increased by 76.0%, 107.0%, 216.1%, 150.0%, 350.9% and 97.1%, respectively, and the malondialdedyde content decreased by 46.4%. It was suggested that low concentration (0.1-0.5 mmol·L-1) SNP could alleviate the toxic effect of acid rain stress on longan seedlings via activating the  leaf antioxidant enzyme activities and reducing oxidative stress, while high concentration SNP (1.0 mmol·L-1) lowered the mitigation effect.
    Effects of topographical condition and sampling number on the interpolation precision of forest litter carbon density.
    ZHANG Jia-jia1,2, FU Wei-jun1,2, DU Qun3, ZHANG Guo-jiang3, JIANG Pei-kun1,2
    2013, 24(8):  2241-2247. 
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    The territory of Zhejiang Province, East China was grouped into 3 topographical units (plain-coastal area, hill-basin area, and mountain area) to investigate the effects of topographical condition and sampling number on the Kriging interpolation precision of forest litter carbon density in the Province. The forest litter carbon density in the 3 topographical units showed a medium spatial correlation pattern, with the semi-variance nugget/sill ratio value ranged from 28.3% to 72.4%. The Kriging interpolation precision was in the order of plain-coastal area > hill-basin area > mountain area, indicating that the Kriging interpolation precision decreased with the increase of terrain complexity degree. Within the same topographical units, the Kriging interpolation precision improved with increasing sampling number, being most obvious in the mountain area. Therefore, under complicated topographical conditions, greater sampling number was required to achieve a high precision of Kriging interpolation.
    Retrieval of leaf area index of Moso bamboo forest with Landsat Thematic Mapper image based on PROSAIL canopy radiative transfer model.
    GU Cheng-yan1,2, DU Hua-qiang 1,2, ZHOU Guo-mo1,2, HAN Ning1,2, XU Xiao-jun1,2, ZHAO Xiao1,2, SUN Xiao-yan1,2
    2013, 24(8):  2248-2256. 
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    The PROSAIL canopy radiative transfer model was used to establish leaf area index (LAI) and canopy reflectance lookuptable for Moso bamboo forest. The combination of Landsat Thematic Mapper (TM) image and this model was then used to retrieve LAI. The results demonstrated that the sensitivity of the input parameters in the PROSAIL model decreased in order of LAI>chlorophyll content (Cab) > leaf structure parameters (N) > mean leaf angle (ALA) > equivalent water thickness (Cw) > dry matter content (Cm). The most sensitive factors LAI and Cab were then used to construct the LAIcanopy reflectance lookup-table. The LAI estimates from the PROSAIL model had good agreement with the reference data, with the coefficient of determination (R2) reached 0.90. The root mean square error (RMSE) and relative RMSE were 0.58 and 13.0%, respectively. However, the mean LAI estimate was higher than the observed value.
    Estimation of shelter forest area in ThreeNorth Shelter Forest Program region based on multisensor remote sensing data.
    ZHENG Xiao1,2,3, ZHU Jiao-jun1,2
    2013, 24(8):  2257-2264. 
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    The Three-North Shelter Forest Program is a key forestry ecological project in China. The quantity and spatial distribution of the shelter forest in the program affect the ecological environment of the entire Three-North region. In this paper, multisensor remote sensing data were used to scientifically, objectively and comprehensively estimate the quantity and spatial distribution pattern of the shelter forest in this region in 1978-2008. Firstly, the Landsat TM images (30 m in resolution) were adopted to extract the shelter forest data in this region in 2008. Then, based on random sampling techniques, the calibration formulae for the shelter forest area in different precipitation climate regions estimated by the SPOT5 (2.5 m in resolution) and Landsat TM were constructed. By using the above-mentioned results, the shelter forest area in the ThreeNorth region in 2008 was estimated. In 2008, the total area of the shelter forest (canopy density of arbor shelter forest was >0.3, coverage of shrub shelter forest was > 40%, and accuracy was about 85%) in this region was 328360.03 km2, with 116244.55 km2 in Northeast China, 42981.32 km2 in North China, 76767.05 km2 in Loess Plateau, and 92367.11 km2 in MongoliaXinjiang Region. According to the classification of shelter forest types, the areas of coniferous forest, broadleaved forest, mixed broadleafconifer forest and shrubland were 62614.74, 121628.51, 22144.09 and 121972.69 km2, respectively.
    Effects of dynamic aerodynamic parameters on simulating the land-atmosphere flux exchange in maize field: A case study of BATSle model.
    CAI Fu1, MING Hui-qing2, LI Rong-ping1, ZHOU Guang-sheng3
    2013, 24(8):  2265-2273. 
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    Based on the continuous observations on the land-atmosphere flux exchange and the meteorological and biological elements in a maize field at the Jinzhou Agricultural Ecosystem Research Station in Liaoning Province of Northeast China from 2006 to 2008, and by using the dynamic roughness (z0) and zerodisplacement (d) parameterization scheme considering the effects of leaf area index, canopy height and wind speed at different developmental stages of maize, the BATS1e model was modified, and applied to investigate the effects of dynamic aerodynamic parameters on the flux exchange between maize agroecosystem and atmosphere.  Compared with the original model, the drag coefficient (CD) simulated by the modified model increased, and its diurnal variation was more obvious with increasing vegetation coverage, which was more accordant with practical circumstances. The simulation accuracies of sensible heat (H), latent heat (λE) and soil heat flux were improved in varying degree, and the NashSutcliffes (NSs) were increased by 0.0569, 0.0194 and 0.0384, with the improvement quantities in the growth season being 0.9%, 1.1% and 1.2% of global radiation, respectively. The dynamic parameterizations of z0 and d played a more remarkable role to increase the simulation accuracies of H and λE with the actual observation of soil water content introduced into the improved model. This research proved that more reasonable dynamic aerodynamic parameterizations could fulfill an obvious function to improve the land surface processes simulation.
    Suitability assessment of construction land in the central and southern parts of Hebei Province, China based on potential-limitation model.
    YIN Hai-wei1, KONG Fan-hua2, LUO Zhen-dong1, YAN Wei-jiao2, SUN Chang-feng2, XU Feng2
    2013, 24(8):  2274-2280. 
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    The suitability assessment of regional construction land is one of the important prerequisites for the spatial arrangement in regional planning, and also, the important foundation for the reasonable utilization of regional land resources. With the support of GIS, and by using the regional comprehensive strength and spatial accessibility analysis and the ecoenvironmental sensitivity analysis, this paper quantitatively analyzed the development potential and its ecological limitation of the central and southern parts of Hebei Province. Besides, based on the cost-benefit analysis, the potential-limitation model was accordingly developed, and the three land suitability scenarios under different developmental concepts were captured through the interaction matrix. The results indicated that both the comprehensive strength and the development potential of the study area showed a primacy distribution pattern, and presented an obvious pole-axis spatial pattern. The areas with higher eco-environmental sensitivity were mainly distributed in the west regions, while those with lower eco-environmental sensitivity were in the east regions. Regional economic development concept had important effects on the regional ecological security pattern and urban growth. The newly developed principles and methods for the land suitability assessment in this paper could not only scientifically realize the spatial grid of regional development potential and capture the future land development trend and spatial distribution, but also provide scientific basis and effective ways for urban and regional planning to realize region ‘smart growth’ and ‘smart conservation’.
    Diversity of soil bacterial community in banana orchards infected with wilt disease.
    CHEN Bo1,2, HUANG Xiao2, LIU Xiao-yu2, ZHOU Deng-bo2, TAN Xin2, GAO Zhu-fen2, ZHANG Xi-yan2, QI Chun-lin1
    2013, 24(8):  2281-2286. 
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    Six soil samples including 3 wilt disease-infected samples and 3 disease-free samples were collected from the banana orchards in 3 areas in Lingao County, Hainan Province of South China. The soil physical and chemical properties were determined by conventional methods, and the diversity of soil bacterial community was analyzed by terminal restriction fragment length polymorphism (T-RFLP). Then, the relationships between the soil bacterial community composition and the soil physical and chemical properties were investigated. In the same areas, most of the soil physical and chemical properties were poorer in diseaseinfected than in diseasefree banana orchards, with the most obvious difference in soil available P content and pH. The T-RFLP analysis showed the diversity of soil bacterial community was richer in disease-infected than in diseasefree banana orchards. The lengths of the dominant T-RFs in the 3 areas were 144, 147 and 233 bp, respectively. Through the comparison with phylogenetic assignment tool, it was deduced that the dominant species in the 3 areas were Bacillus subtilis, Staphylococcus and Eubacterium ruminantium. The distribution of most T-RFs was related to the soil alkaline hydrolyzable N, available K, available P and water content, and the relative abundance of most T-RFs was richer in disease-infected than in disease-free banana orchards.
    Effects of actinomycetes agent on ginseng growth and rhizosphere soil microflora.
    ZHANG Hong-yan1,2, XUE Quan-hong1, SHEN Guang-hui1, WANG Dong-sheng1
    2013, 24(8):  2287-2293. 
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    Taking the ginseng in Xiao Xing’an Mountains of Northeast China as test object, this paper studied the effects of applying Streptomyces pactum (Act12) on ginseng growth and on the soil microflora in root zone and root surface. After treated with Act12, the yield and quality of ginseng’s medicinal part improved, the induced enzyme activities in leaves and the root activity increased, and the numbers and proportions of soil bacteria and actinomycetes increased significantly while those of soil fungi decreased.  Compared with the control, the soil microflora in treatment Act12 changed. The numbers of the dominant bacteria Pseudomonas fluorescens, Pseudomonas koreensis, and Microbacterium oxydans were much higher in root zone soil and root surface soil, and the pathogen Plectosphaerella cucumerina decreased in root zone soil and disappeared in root surface soil. These results suggested that the addition of Act12 could improve the soil microflora, enhance the resistance and root activity of ginseng plant, and increase the ginseng yield and its quality.
    Screening, identification and phosphate-solubilizing characteristics of Rahnella sp. phosphate-solubilizing bacteria in calcareous soil.
    QIAO Zhi-wei, HONG Jian-ping, XIE Ying-he, LI Lin-xuan
    2013, 24(8):  2294-2300. 
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    Several strains of phosphate-solubilizing bacteria were isolated and screened from the crop rhizosphere of calcareous soil in Shanxi Province of China. After repeated isolation and purification, the strain W25 with strong phosphate-solubilizing activity was obtained, and identified as Rahnella sp., based on the morphological, physiological and biochemical properties and the analysis of 16S rRNA gene sequence. Further studies on the W25 showed that the maximum phosphate solubilizing capability of the W25 on tricalium phosphate, aluminum phosphate and ferric phosphate reached 385.5, 110.4 and 216.6 mg·L-1, respectively. In the liquid culture with aluminum phosphate and ferric phosphate, the solubilized phosphorous by the W25 was significantly negatively correlated with the liquid pH, with the correlation coefficient being 0.56 and 0.81, respectively. Among the carbon and nitrogen sources, glucose and ammonium nitrate were the optimum for the solubilization of tricalium phosphate by W25. The utilization of carbon source was in the order of glucose > lactose > sucrose > mannitose > starch, and that of nitrogen source was in the order of ammonium nitrate > ammonium chloride > ammonium sulfate > potassium nitrate > sodium nitrate. Different nitrogen sources had greater effects on the production of organic acids by W25. Formic acid and acetic acid would be produced when the nitrogen source was NH4+, oxalic acid and succinic acid would be produced when the nitrogen source was NO3-, and citric acid would be extra produced when the ammonium nitrate was used as the nitrogen source.
    Characteristics of soil microbial community under different vegetation types in Wuyishan National Nature Reserve, East China.
    WU Ze-yan1,2, LIN Wen-xiong2, CHEN Zhi-fang3, FANG Chang-xun2, ZHANG Zhi-xing2, WU Lin-kun2, ZHOU Ming-ming2, SHEN Li-hua2
    2013, 24(8):  2301-2309. 
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    By using Biolog Ecoplate system, this paper studied the structure and functional diversity of soil microbial community under different vegetation types in Wuyishan National Nature Reserve, aimed to probe into the effects of vegetation type on the  diversity of soil microbial community. The results showed that the soil chemical properties, soil enzyme activities, and average well color development (AWCD) were higher in natural forest than in planted forest, and were the lowest in abandoned field. The AWCD reflecting soil microbial activity and functional diversity was increased with increasing incubation time, but there existed significant differences among different vegetation types. The carbon sources mostly used by soil microbes were carbohydrates and carboxylic acids, followed by amino acids, phenolic acids and polymers, and amines had the lowest utilization rate. The Simpson index, Shannon index, Richness index and McIntosh index in natural forest were holistically higher than those in planted forest. Principal component analysis (PCA) identified 2- principal component factors in relation to carbon sources, explaining 56.3% and 30.2% of the variation, respectively. The carbon sources used by soil microbial community differed with vegetation types. Amino acids and amides were the two main carbon sources separating the 2 principal component factors.  The results of this study could provide basis for further approaching the relationships between vegetation diversity and soil microbial community diversity.
    Abundance and biomass of planktonic ciliates in the shelf of East China Sea in spring and autumn.
    YU Ying1,2, ZHANG Wu-chang1, ZHOU Feng3, LIU Cheng-gang3,4, FENG Mei-ping1,2, LI Hai-bo1,2, ZHAO Yuan1, XIAO Tian1
    2013, 24(8):  2310-2318. 
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    An investigation was made on the abundance and biomass of planktonic ciliates in the shelf of East China Sea in May (spring) and November (autumn), 2011. The abundance of the ciliates in spring and autumn was averagely (614±861) and (934±809) ind·L-1, and the biomass was averagely (1.70±3.91) and (0.93±0.99) μg C·L-1, respectively. The high abundance and biomass in spring were found in coastal and offshore areas, and those in autumn were in offshore only. In the two seasons, the ciliates tended to accumulate in the waters upper layer, and sometimes flocked in the bottom. In the spring, aloricate ciliate species were larger than those in the autumn. Tintinnids occupied (26.9%±34.3)% and (44.9%±25.2)% of the total ciliates abundance in spring and autumn, respectively. In taxonomy, 52 tintinnid species of 27 genera were identified. The most dominant species were Tintinnidium primitivum, Stenosemella oliva, and Tintinnopsis tubulosoides in spring, and Tintinnidium primitivum, Stenosemella parvicollis, and Tintinnopsis nana in autumn. The ciliates abundance showed significant positive correlations with water temperature and Chl a concentration, the tintinnids abundance showed significant negative correlation with water salinity, and the tintinnids community was significantly related to water temperature.
    Acute toxicity of different type pesticide surfactants to Daphnia magna
    LI Xiu-huan, LI Hua, CHEN Cheng-yu, LI Jian-tao, LIU Feng
    2013, 24(8):  2319-2324. 
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    By using the standard test methods in Experimental Guideline for Environmental Safety Evaluation of Chemical Pesticide to aquatic organisms, a comparative study was conducted on the acute toxicity of 39 nonionic, 6 anionic, and 3 cationic surfactants to Daphnia magna. The acute toxicity of three cationic surfactants  1427, 1227 and C8-10 to D. magna belonged to virulent level, and the toxicity of 1427 was the highest, with the EC50 value being 0.97x10-2 mg·L-1. The acute toxicity of nonionic surfactants polyoxyethylene ether castor oil EL, Tween, and Span emulsifiers belonged to low level, but the toxicity of alkylphenol polyoxyethylene ether and fatty alcohol polyoxyethylene ether surfactants was relatively high, of which, AEO-7 and AEO-5 displayed high toxicity, with the EC50 value being 0.82 and 0.97 mg·L-1, respectively. In these surfactants, the more liposolubility, the higher the toxicity was. Most of the anionic surfactants were medium in toxicity, but the acute toxicity of NNO belonged to high toxicity, with the EC50 value being 0.17 mg·L-1.
    Predation and removal of rodents on the seeds with different size and pericarp traits.
    ZHOU Li-biao, YAN Xing-fu, WANG Jian-li, ZHOU Yun-feng
    2013, 24(8):  2325-2332. 
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    A field survey was conducted in the Quercus wutaishanica shrubs in Liupan Mountains of Ningxia, Northwest China to study the predation and removal of rodents on the seeds of Q. wutaishanica, Prunus salicina and Pinus armandii, aimed to explore the effects of seed size and pericarp traits on the predation and removal behaviors of rodents. The in situ seed predation rates of smaller Q. wutaishanica seeds and P. armandii seeds were significantly higher than those of larger Q. wutaishanica seeds and P. salicina seeds. The P. salicina seeds with hard and thick pericarp (endocarp) had the highest predation rate and hoarding rate after the removal by rodents. The movement distance of larger Q. wutaishanica seeds during predation events was the longest (3.10 m), and the seed hoarding distance of this species (6.48 m) was significantly longer than that of the three other types of seeds. Over 80% of sites were used as the predation sites by rodents for the seeds, except that the P. salicina seeds contained only a single seed and the cache sites contained a single seed accounted for over 90% for all types of seeds. Few predation and cache sites containing over two seeds were detected. Higher proportion of P. armandii seeds were predated in micro-habitats except at the base of shrubs and in the holes after removal by rodent, while the seeds of other three types were predated mainly at the base of shrubs and in the holes after removal by rodents. The seed hoarding patterns after removal by rodents were primarily determined by pericarp traits, and higher proportion of soil burial that the rodents hoarded seeds with hard pericarp (endocarp) was detected.
    Bird species diversity and related protection measures in urban park green spaces of Loudi City, Hunan Province of China.
    LI Yi-de1, LIU Ping-yuan2, GONG Xun-sheng3, XIAO Xiao-jun3
    2013, 24(8):  2333-2338. 
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    Urban park green space is an important physical part of urban ecosystem, and also, the important habitat and carrier for birds and other animals. Rapid urbanization induces the great change in the spatial pattern of urban park green space, while the patched distribution of urban park green space has the habitat features similar to ‘habitat islands’, giving obvious effects on urban avian communities. In order to understand the bird species distribution and species diversity in Loudi City and to provide the basic information for the bird conservation, a line transect method and a quadrat sampling method were adopted to investigate the distribution pattern and species richness of the birds across seven urban parks in the Loudi City from November, 2010 to January, 2012. A total of 56 birds species belonging to 11 orders and 27 families were recorded, among which, there were 32, 12 and 12 species belonging to resident birds, summer migrant birds and winter migrant birds, accounting for 57.2%, 21.4% and 21.4%, respectively. As for the fauna, there were 27, 14, and 15 bird species belonging to oriental species, palaearctic species and widely distributed species, accounting for 48.2%, 25.0% and 26.8%, respectively. A total of 7 species belonging to the second class of the national key protected species were recorded, accounting for 12.5% of the total. The Shannon, Pielou and G-F indices of the bird communities in the urban parks in Loudi City were 1.49, 0.85 and 0.62, respectively. Zhushan Park had the highest species number (42), Shannon index (1.41), G index (3.46), F index (6.12) and G-F index (0.43), and Yueqin Hill Park had the highest Pielou index (0.92). The reasons of the poor bird species in Loudi City were analyzed, and some suggestions for preventing the birds were put forward.
    Effects of rice straw returning on the community structure and diversity of nitrogen-fixing gene (nifH) in paddy soil.
    ZHANG Miao-miao1,2,3, LIU Yi1,2, SHENG Rong1,2, QIN Hong-ling1,2, WU Yan-zheng1,2,3, WEI Wen-xue1,2
    2013, 24(8):  2339-2344. 
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    Taking a long-term fertilization experiment in Taoyuan Agro-ecosystem Research Station under Chinese Academy of Sciences as the platform, and selecting four treatments (no fertilization, CK; rice straw returning, C; nitrogen, phosphorus and potassium fertilization, NPK; and NPK+C) as the objects, soil samples were collected at the tillering, booting and maturing stages of rice, and the abundance, composition and diversity of nifHcontaining bacterial community were measured by realtime quantitative PCR and terminal restriction fragment length polymorphism (T-RFLP), aimed to understand the effects of rice straw returning on the nifHcontaining bacterial community in paddy soil.  Compared with CK, treatments NPK+C and NPK increased the abundance of nifHcontaining microorganisms significantly (except at tillering stage), and NPK+C had the highest abundance of nifHcontaining microorganisms. Under the effects of longterm fertilization, the composition of nifH gene community in CK differed obviously from that in the other three treatments. The nifH composition had definite difference between C and NPK, but less difference between NPK and NPK+C. Longterm fertilization did not induce significant changes in nifH diversity. Therefore, longterm rice straw returning not only induced the changes of nifH gene community composition, but also resulted in a significant increase in the abundance of nifHcontaining community, and hence, the increase of soil nitrogen fixing capacity.
    Population genetic structure and interspecific introgressive hybridization between Camellia meiocarpa and C. oleifera.
    HUANG Yong
    2013, 24(8):  2345-2352. 
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    A total of 11 pairs of SSR primers were applied to study the genetic diversity of five sympatric populations of Camellia meiocarpa and C. oleifera, the populations’ genetic structure, and the interspecific introgressive hybridization. The alleles shared by the two species were 57, accounting for 96.6% of the total. There were two specific alleles in C. oleifera, but no specific alleles in C. meiocarpa. Certain difference was observed in the phenotypic frequency between the two species, and the intraspecific genetic diversity of the two species was high. At species level and population level, the polymorphism information content of the two species was between 0.25 and 0.50, suggesting that the genetic polymorphism was moderate. The mean value of genetic differentiation among the sympatric species was 0.057, being obviously lower than that of the foreign species (C. meiocarpa 0.332; C. oleifera 0.201) and of xyloid angiosperms (0.102). The mean value of gene flow among the sympatric species was 10.072, which was significantly higher than that among the foreign species (C. meiocarpa 1.006, C. oleifera 1.990). The UPGMA cluster results showed that the genetic relationship of the two species was very close to, meaning that there existed an obvious interspecific introgressive hybridization. C. meiocarpa was possibly a variety of C. oleifera as a result of the introgressive hybridization.
    Ecological niche breadth and niche overlap of dominant species of fish assemblage in Yangtze River estuary and its adjacent waters.
    LI Xian-sen1, YU Zhen-hai2, SUN Shan1, JIN Xian-shi1
    2013, 24(8):  2353-2359. 
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    Based on the fishery resources data from the bottom trawl surveys conducted on the R/V Beidou in the Yangtze River estuary and its adjacent waters in June, August and October of 2006, the index of relative importance (IRI) was measured to determine the dominant species of fish assemblage, and the niche indicators and their seasonal variations of the dominant species were analyzed. A total of 10 dominant species in the 3 survey cruises were recorded, which were divided into two groups by the Braycurtis similarity clustering and nonmetric multidimensional scaling (MDS) analysis, with a significant seasonal variation of niche breadth and niche overlap. One group included Engraulis japonicus, Champsodon capensis, and Acropoma japonicum, whose niche breadth and niche overlap were larger in summer than in autumn, with a migration from the Yangtze River estuary and its adjacent waters to outer deeper waters, while the other group included Trichiurus haumela, Chaeturichthys stigmatias, Apogon lineatus, Larimichthys polyactis, Psenopsis anomala, Argyrosomus argentatus, and Benthosema pterotum, whose niche breadth and niche overlap were larger in autumn than in summer, with a reverse migration from southern Yellow Sea and northern East China Sea to the Yangtze River estuary and its adjacent waters. The different migration direction of the two groups was related to their ecological habits and environmental factors.
    Evolution and discrimination of ecological planning and its related conceptions.
    HE Xuan1, MAO Hui-ping1, NIU Dong-jie1, BAO Cun-kuan2
    2013, 24(8):  2360-2368. 
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    Ecological planning is one of the most important tools in realizing city’s sustainable development. The ecological planning in China was started in the 1980s, and on the basis of assimilating the existing theoretical and practical experiences from western countries, basically formed a theoretical system in accordance with the Chinese characteristics and acquired a series of practical results. This paper reviewed the research and development processes of China’s ecological planning. It is considered that the study of our ecological planning was derived from the geographical principles of land resources ecological utilization, the ecological principles of complex ecological system theory, and the combination of these two principles. The ecological planning has experienced three research stages, i.e., singledisciplinary exploration, multi-disciplinary and multiperspective study (including landscape ecology, ecology and urban planning), and interdisciplinary and multi-disciplinary collaboration research. The ecological planning and the related conceptions, primary principles, main academic points, and representatives at each research stage were summarized, and through the discrimination of the basic conceptions of ecological planning and other related plans, it was pointed out that ecological planning is an general conception which includes land ecological planning, urban ecological planning, and eco-city planning, and the principles and theories of ecological planning should be integrated into, led and restricted the present planning system to promote the city’s and regional  sustainable development.
    Structure and function of arbuscular mycorrhiza: A review.
    TIAN Mi1, CHEN Ying-long2, LI Min1, LIU Run-jin1
    2013, 24(8):  2369-2376. 
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    Arbuscular mycorrhiza (AM) is one of the most widely distributed and the most important mutualistic symbionts in terrestrial ecosystems, playing a significant role in enhancing plant resistance to stresses, remediating polluted environments, and maintaining ecosystem stabilization and sustainable productivity. The structural characteristics of AM are the main indicators determining the mycorrhizal formation in root system, and have close relations to the mycorrhizal functions. This paper summarized the structural characteristics of arbuscules, vesicles, mycelia and invasion points of AM, and analyzed the relationships between the Arum (A) type arbuscules, Paris (P) type arbuscules, vesicles, and external mycelia and their functions in improving plant nutrient acquisition and growth, enhancing plant resistance to drought, waterlogging, salinity, high temperature, diseases, heavy metals toxicity, and promoting toxic organic substances decomposition and polluted and degraded soil remediation. The factors affecting the AM structure and functions as well as the action mechanisms of mycorrhizal functions were also discussed. This review would provide a basis for the systemic study of AM structural characteristics and functional mechanisms and for evaluating and screening efficient AM fungal species.
    Research advances on anaerobic ferrous-oxidizing microorganisms.
    ZHANG Meng, ZHENG Ping, JI Jun-yuan
    2013, 24(8):  2377-2382. 
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    Anaerobic ferrous-oxidizing microorganisms (AFOM) are one of the important discoveries in microbiology, geology and environmental science. The study of AFOM is of significance to make clear the banded iron formations (BIFs), promote the biogeochemical cycles of iron, nitrogen and carbon, enrich the microbiological content, develop new biotechnologies for anaerobic iron oxidation, and explore the ancient earth environment and extraterrestrial life. This paper summarized the research advances on AFOM, introduced the habitats of AFOM, discussed the biodiversity and the nutritive and metabolic characteristics of AFOM, and assessed the potential functions of AFOM. An outlook was made on the future researches of new species AFOM, their microbial metabolism mechanisms, and their development and applications.
    Research progress in post-fire debris flow.
    DI Xue-ying1, TAO Yu-zhu1,2
    2013, 24(8):  2383-2392. 
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    The occurrence of the secondary disasters of forest fire has significant impacts on the environment quality and human health and safety. Post-fire debris flow is one of the most hazardous secondary disasters of forest fire. To understand the occurrence conditions of postfire debris flow and to master its occurrence situation are the critical elements in postfire hazard assessment. From the viewpoints of vegetation, precipitation threshold and debris flow material sources, this paper elaborated the impacts of forest fire on the debris flow,  analyzed the geologic and geomorphic conditions, precipitation and slope condition that caused the post-fire debris flow as well as the primary mechanisms of debris-flow initiation caused by shallow landslide or surface runoff, and reviewed the research progress in the prediction and forecast of post-fire debris flow and the related control measures. In the future research, four aspects to be focused on were proposed, i.e., the quantification of the relationships between the fire behaviors and environmental factors and the postfire debris flow, the quantitative research on the postfire debris flow initiation and movement processes, the mechanistic model of postfire debris flow, and the rapid and efficient control countermeasures of post-fire debris flow.