Antifeedant and antioviposition activities of Momordica charantia leaf ethanol extract against Liriomyza sativae.
LING Bing1;XIANG Ya-lin1,2;WANG Guo-cai3;CHEN Shao-hua 1;ZHANG Mao-xin1
2009, 20(04):
836-842 .
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1982 )
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Liriomyza sativae Blanchard (Diptera: Agromyzidae) is one of the important pests harming a wide variety of vegetables and ornamental plants throughout the world. The leaf ethanol extract of Momordica charantia at the concentration of 2000〖KG-*2〗-〖KG-*7〗4000 μg·ml-1 displayed significant antifeedant and antioviposition activities against L. sativae adults. For further purifying the extract, four solvents, i.e., cyclohexane, ethyl acetate, n-butanol and water, were used to extract the ethanol extract, and the antifeedant and antioviposition activities of the extracts against L. sativae adults were te sted. The results showed that after treated with the extracts at the concentration of 1000 μg·ml-1 for 2 days, the antifeedant index (AFI) of cyclohexane-, ethyl acetate-, n-butanol- and water extracts against L. sativae adults was 1108%, 3489%, 2299% and 0, and the antioviposition index (AOI ) was 0, 3091%, 645% and 0, respectively. Ethyl acetate extract had the highest bioactivity. At the concentration of 4000 μg·ml-1, the AFI and AOI of ethyl acetate extract were 7095% and 6949%, respectively. The ethyl acetate extract was then isolated by silica gel column chromatography, a nd three compounds, i.e., (19S, 23E)-5β,19-epoxy-19-methoxy-cucurbita-6,23-dien-3β and 25-diol (compound 1), (19R, 23E)-5β,19-epoxy-19-methoxy-cucurbita-6,23-dien-3β and 25-diol (compound 2), and 3β,7β,25-trihydroxycucurbita-5,23-dien-19-al-3-O-β-D-glucopyranoside (compound 3), were obtained. These three compounds at concentration of 100〖KG-*2〗-〖KG-*7〗400 μg·ml-1 all had inhibitory effects on the feeding and oviposition of L. sativae. At the concentration of 400 μg·ml-1, the AFI and AOI were 6689%, 5353% and 7802%, and 7632%, 5836% and 7836% for compound 1, 2 and 3, respectively.