24-表油菜素内酯和盐度对浒苔生长和生理活性的影响

doi:10.13287/j.1001-9332.201603.032

应用生态学报 ›› 2016, Vol. 27 ›› Issue (3): 946-#REF.doi: 10.13287/j.1001-9332.201603.032

24-表油菜素内酯和盐度对浒苔生长和生理活性的影响

王东^1,2, 李亚鹤^1,2, 徐年军^1,2*, 徐晓婷^1,2

¹宁波大学教育部应用海洋生物技术重点实验室, 浙江宁波 315211;
²宁波大学浙江省海洋生物工程重点实验室, 浙江宁波 315211

收稿日期:2015-07-15 出版日期:2016-03-18 发布日期:2016-03-18
通讯作者: * E-mail: xunianjun@nbu.edu.cn
作者简介:王东,男,1991年生,硕士研究生.主要从事藻类生理生态学研究.E-mail:qhr1018@126.com
基金资助:
本文由国家自然科学基金项目(40876073,41276122)、教育部博士点基金项目(20123305110002)、浙江省自然科学基金项目(421501940 )、宁波大学科研启动项目(F01259144702 )和宁波大学学科项目 (XKL14D2085)资助

Combined effects of 24-epibrassinolide and salinity on the growth and physiological perfor-mance of Ulva prolifera

WANG Dong^1,2, LI Ya-he^1,2, XU Nian-jun^1,2*, XU Xiao-ting^1,2

¹Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo 315211, Zhejiang, China;
²Key Laboratory of Marine Biotechnology of Zhejiang, Ningbo 315211, China

Received:2015-07-15 Online:2016-03-18 Published:2016-03-18
Contact: * E-mail: xunianjun@nbu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (40876073, 41276122),Doctoral Fund of Ministry of Education of China (20123305110002), Natural Science Foundation of Zhejiang Pro-vince (421501940), Scientific Research Project of Ningbo University (F01259144702) and Discipline Project of Ningbo University (XKL14D2085).

摘要/Abstract

摘要： 为探讨低温条件下外源植物激素和盐度变化对浒苔光合生理的影响,本文选取浒苔为供试材料,研究24-表油菜素内酯和盐度对其生长、光合色素、叶绿素荧光参数、抗氧化活性和可溶性蛋白含量的影响.结果表明: 与正常盐度(近岸海水盐度为25)相比,浒苔的生长在盐度10处理下显著增加,增幅为45.9%,但在盐度5处理下显著降低.盐度5处理的浒苔比其他盐度处理具有较高的Chl a和可溶性蛋白.24-表油菜素内酯(0.2 mg·L^-1)的加入显著抑制了浒苔的生长,尤其在盐度25处理下,浒苔呈现负增长的趋势,并大量释放孢子(配子),有效光化学效率、超氧化物歧化酶酶活性和可溶性糖含量明显降低,但可溶性蛋白含量显著增加.即在温度15 ℃条件下,可通过适当降低盐度促进浒苔的生长;在盐度25条件下,可通过添加24-表油菜素内酯促进孢子(配子)释放,为浒苔的大规模养殖提供原材料.

关键词: 盐度, 24-表油菜素内酯, 抗氧化活性, 叶绿素荧光效率, 浒苔

Abstract: In order to study the combined effects of brassinosteroids and salinity on the growth and physiological performance of Ulva prolifera under low temperature condition, we investigated the growth, chlorophyll content, chlorophyll fluorescence parameters, soluble protein and carbohydrate contents in this algae, which was grown under three salinity in the presence or absence of 24-epibrassinolide. The results showed that, compared to control salinity (25) treatment, the growth rate of U. prolifera was enhanced by 45.9% under the moderate hyposaline condition (10), but decreased under low salinity (5) treatment, which showed high contents of chlorophyll and soluble protein. However, the presence of EBR (0.2 mg·L^-1) significantly reduced the growth of U. prolifera, especially under the control salinity (25) treatment, under which the fresh mass decreased and more spores were released. Additionally, the effective quantum yield (F_v′/F_m′), the activity of SOD and the content of soluble carbohydrate also decreased, but the soluble protein content increased under the control salinity treatment in the presence of EBR. In conclusion, moderatehypo-saline condition could be used to enhance the growth of U. prolifera at 15 ℃, and under normal salinity (25), the EBR could be used to promote the release of spores and produce more materials for mass production of U. prolifera.

Key words: salinity, antioxidant, Ulva prolifera, 24-epibrassinolide, chlorophyll fluorescence parameters

王东, 李亚鹤, 徐年军, 徐晓婷. 24-表油菜素内酯和盐度对浒苔生长和生理活性的影响[J]. 应用生态学报, 2016, 27(3): 946-#REF.

WANG Dong, LI Ya-he, XU Nian-jun, XU Xiao-ting. Combined effects of 24-epibrassinolide and salinity on the growth and physiological perfor-mance of Ulva prolifera[J]. Chinese Journal of Applied Ecology, 2016, 27(3): 946-#REF.

参考文献

[1]Wu H-X (吴洪喜), Xu A-G (徐爱光), Wu M-N (吴美宁). Preliminary study on experiment ecology of E. prolifera. Journal of Zhejiang Ocean University (Natural Science)(浙江海洋学院学报:自然科学版), 2000,19(3): 230-234 (in Chinese)
[2]Wang J-W (王建伟), Yan B-L (阎斌伦), Lin A-L (林阿朋), et al . Ecological factor research on the growth and induction of spores release in Enteromorpha prolifera . Marine Science Bulletin(海洋通报), 2007,26(2): 60-65 (in Chinese)
[3]Taylor R, Fletcher RL. A simple method for the freeze-preservation of zoospores of the green macroalga Enteromorpha prolifera . Journal of Applied Phycology,1999,11: 257-262
[4]Finlay JA, Callow ME, Linnea KI, et al.The influence of surface wettability on the adhesion strength of settled spores of the green alga Enteromorphaand the diatom Amphora . Integrative and Comparative Biology,2002,42: 1116-1122
[5]Zhang H-Y (张寒野), Wu W-X (吴望星), Song L-Z (宋丽珍), et al.Trial and field cultivation ofEnteromorpha clathrataand effects of ecological factors on its growth. Journal of Fishery Sciences of China(中国水产科学), 2006,13(5): 781-786 (in Chinese)
[6]Edwards DM, Reed RH, Stewart WDP. Osmoacclimation in Enteromorpha intestinalis: Long-term effects of osmoticstress on organic solute accumulation.Journal of Experimental Marine Biology and Ecology,1988, 98: 467-476
[7]Lin AP, Wang C, Pan GH, et al . Diluted seawater promoted the green tide of Ulva prolifera . Phycological Research,2011,59: 295-304
[8]Sasse JM. Recent progress in brassinosteroid research. Physiological Plant Pathology,1997,100: 696-701
[9]Bajguz A, Hayat S. Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiology and Biochemistry,2009,47: 1-8
[10]Vriet C, Russinova E, Reuzeau C. Boosting crop yields with plant steroids. Plant Cell,2012,24: 842-857
[11]Zhang Y-P (张永平), Chen Y-Y (陈幼源), Yang S-J (杨少军). Effects of 2,4- epibrassinolide on physiological characteristics and photosynthesis of melon seedlings under high temperature stress. Plant Physiology Journal(植物生理学报), 2012,48(7): 683-688 (in Chinese)
[12]Ogweno JO, Song XS, Shi K, et al . Brassinosteroids alleviate heat-induced inhibition of photosynthesis by increasing carboxylation efficiency and enhancing antioxidant systems in Lycopersicon esculentum . Journal of Plant Growth Regulation,2008,27: 49-57
[13]Wu X (吴秀), Lu X-M (陆晓民). Effects of brassinolide on the antioxidant system and photosynthesis of cucumber seedlings under suboptimal temperature, light and salt environment. Chinese Journal of Applied Ecology(应用生态学报), 2015,26(9): 2751-2757 (in Chinese)
[14]Li N (李宁), Guo S-R (郭世荣), Shu S (束胜), et al . Effects of exogenous 24 -epibrassinolide on leaf morphology and photosynthetic characteristics of tomato seedlings under low light stress. Chinese Journal of Applied Ecology(应用生态学报), 2015,26(3): 847-852 (in Chinese)
[15]Li J (李静), Wang Q-Q (王俏俏), Xu N-J (徐年军), et al . Effect of 24- epibrassinolide on the resistance of Gracilaria lemaneiformisto high temperature. Acta Oceanologica Sinica(海洋学报), 2014,36(8): 82-90 (in Chinese)
[16]Hu W (胡伟), Sun X (孙雪), Fan M-H (范美华), et al . Effects of 24- epibrassinolide on phytohormone variation and related physiological response of Ulva prolifera . Oceanologia et Limnologia Sinica(海洋与湖沼), 2014,45(5): 1072- 1077 (in Chinese)
[17]Provasoli L. Media and prospects of the cultivation of marine algae// Watanabe A, Hattori A, eds. Cultures and Collections of Algae. Proceedings of the US-Japan conference held at Hakone, 1968: 63-75
[18]Wellburn AR. The spectral determination of chlorophylls a and b, as well as total cartenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology,1994,144: 307-313
[19]Webb WL, Newton M, Starr D. Carbon dioxide exchange of Alnusrubra: A mathematical model. Oecologia, 1974,17: 281-291
[20]Li H-S (李合生). Principle and Technology of Plant Physiological and Biochemical Experiments. Beijing: Higher Education Press, 2000: 267-268 (in Chinese)
[21]Taylor R, Fletcher RL, Raven JA. Preliminary studies on the growth of selected “Green Tide” algae in laboratory culture: Effects of irradiance, temperature, salinity and nutrients on growth rate. Botanica Marina, 2001,44: 327-336
[22]Fan X, Xu D, Wang YT, et al . The effect of nutrient concentrations， nutrient ratios and temperature on photosynthesis and nutrient uptake by Ulva prolifera: Implications for the explosion in green tides. Journal of Applied Phycology,2014,26: 537-544
[23]Wang Y, Wang Y, Zhu L, et al . Comparative studies on the ecophysiological differences of two green tide macroalgae under controlled laboratory conditions. PLoS ONE,2012,7(8): e38245
[24]Yeo A. Molecular biology of salt tolerance in the context of whole plant physiology. Journal of Experimental Botany,1998,49: 915-929
[25]Gao B-B (高兵兵), Zheng C-F (郑春芳), Xu J-T (徐军田), et al . Physiological responses of Enteromorpha linzaand Enteromorpha proliferato seawater salinity stress. Chinese Journal of Applied Ecology(应用生态学报), 2012,23(7): 1913-1920 (in Chinese)
[26]Luo MB, Liu F. Salinity-induced oxidative stress and regulation of antioxidant defense system in the marine macroalga Ulva prolifera . Journal of Experimental Marine Biology and Ecology,2011,409: 223-228
[27]Shabala SN, Shabala SI, Martynenko AI, et al . Salinity effect on bioelectric activity growth, Na⁺ accumulation and chlorophyll fluorescence of corn leaves: A comparison survey and prospects for screening. Australian Journal of Plant Physiology,1998,25: 609-616
[28]Zhang X-H (张晓红), Wang Z-L (王宗灵), Li R-X (李瑞香), et al. Microscopic observation on population growth and reproduction of Entromorphra proliferaunder different temperature and salinity. Advances in Marine Science(海洋科学进展), 2012,30(2): 276-283 (in Chinese)
[29]Sun L (孙雷), Song X-X (宋秀贤), Bai J (白洁), et al.Impacts of environmental factors and different substrata on the adhesion of Ulva proliferaspores. Perio-dical of Ocean University of China (中国海洋大学学报), 2015,45(5): 059-063 (in Chinese)
[30]Piotrowska-Niczyporuk A, Bajguz A. The effect of natural and synthetic auxins on the growth, metabolite content and antioxidant response of green alga Chlorella vulgaris (Trebouxiophyceae). Plant Growth Regulation,2013,73: 57-66
[31]Park WK, Yoo G, Moon M, et al. Phytohormone supplementation significantly increases growth of Chlamydomonas reinhardtiicultivated for biodiesel production.Applied Biochemistry & Biotechnology, 2013,171: 1128-1142
[32]Wang X-K (王晓坤), Ma J-H (马家海), Ye D-C (叶道才), et al. Preliminary study on the life history of Enteromorpha prolifera . Marine Science Bulletin(海洋通报),2007,26(5): 112-115 (in Chinese)
[33]Chen J (陈静), Shen S-D (沈颂东), Xue J (薛静). Effects of UV radiation and salt stress on the growth of Enteromorpha prolifera. Ecological Science(生态科学 ),2010,29(2): 131-135 (in Chinese)
[34]Ye Q, Zhu W, Li L, et al.Brassinosteroids control male fertility by regulating the expression of key genes involved in Arabidopsisanther and pollen development. Proceedings of the National academy of Sciences of the United States of America,2010,107: 6100-6105

24-表油菜素内酯和盐度对浒苔生长和生理活性的影响

Combined effects of 24-epibrassinolide and salinity on the growth and physiological perfor-mance of Ulva prolifera

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