• 研究论文 •

### 虎斑乌贼的胚胎耗氧率

1. 宁波大学海洋学院, 浙江宁波 315211
• 收稿日期:2015-10-26 发布日期:2016-07-18
• 通讯作者: *E-mail: jiangxiamin@nbu.edu.cn
• 作者简介:王鹏帅,男,1989年生,硕士研究生. 主要从事海洋生物分子育种与健康养殖研究. E-mail: wang_pengshuai@126.com
• 基金资助:
本文由宁波市农业重大专项(2014C11001)和浙江省海洋与渔业项目(浙海渔计2013[82]号)资助

### Oxygen consumption rate of Sepia pharaonis embryos.

WANG Peng-shuai, JIANG Xia-min*, RUAN Peng, PENG Rui-bing, JIANG Mao-wang, HAN Qing-xi

1. School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China
• Received:2015-10-26 Published:2016-07-18
• Contact: *E-mail: jiangxiamin@nbu.edu.cn
• Supported by:
This work was supported by the Major Agriculture Project of Ningbo City, Zhejiang Province, China (2014C11001) and the Ocean and Fishery Project of Zhejiang Province, China (Zhejiang Marine Fishery Program, 2013[82]).

Abstract: This research was conducted to unravel the variation of oxygen consumption rate during different developmental stages and the effects of different ecological factors on embryonic oxygen consumption rate of Sepia pharaonis. The oxygen consumption rates were measured at twelve deve-lopmental stages by the sealed volumetric flasks, and four embryonic developmental periods (oosperm, gastrula, the formation of organization, endoskeleton) were selected under various ecological conditions, such as salinity (21, 24, 27, 30, 33), water temperature (18, 21, 24, 27, 30 ℃) and pH (7.0, 7.5, 8.0, 8.5, 9.0). The results showed that the oxygen consumption rate rose along with the developmental progress, and distinctly differed from each other. The oxygen consumption rate was 0.082 mg·(100 eggs)-1·h-1 during oosperm period, and rose to 0.279 mg·(100 eggs)-1·h-1 during gastrula period, which was significantly higher than that of blastula period. Finally, the oxygen consumption rate rose to 1.367 mg·(100 eggs)-1·h-1 during hatching period. The salinity showed a significant effect on oxygen consumption rate during the formation of organization and endoskeleton formation stage (P<0.05), but no significant effect during oosperm and gastrula periods (P>0.05). The oxygen consumption rates of four studied embryonic stages all rose and then declined along with the increase of salinity, and reached the highest values [0.082, 0.200, 0.768 and 1.301 mg·(100 eggs)-1·h-1, respectively] at salinity 30. The water temperature had a significant effect on the embryo oxygen consumption rates of gastrula, and the formation of organization and endoskeleton formation stage (P<0.05), with the exception of oosperm (P>0.05). The oxygen consumption rates of four studied embryonic stages all rose and then declined along with the increase of temperature, and reached the highest values at 27 ℃ [0.082, 0.286, 0.806 and 1.338 mg·(100 eggs)-1·h-1, respectively]. The pH had no significant effect on the oxygen consumption rates of four embryonic stages (P>0.05). The oxygen consumption rates of four studied embryonic stages all rose and then declined along with the increase of pH. The oxygen consumption rates of gastrula, the formation of organization, endoskeleton reached the according highest values [0.281, 0.799 and 1.130 mg·(100 eggs)-1·h-1] at pH 8.5, but that during oosperm period occurred at pH 8.0 [0.116 mg·(100 eggs)-1·h-1].